Search result: Catalogue data in Autumn Semester 2017

Biology Bachelor Information
3. Year, 5. Semester
Block Courses
Registration for Block courses is mandatory. Please register under Link . Registration period: from 24.7.2017 to 6.8.2017.
Block Courses in 2nd Quarter of the Semester
From 12.10.2017 08:00 Uhr to 3.11.2017 17:00 hr
NumberTitleTypeECTSHoursLecturers
551-1201-00LComputational Methods in Genome and Sequence Analysis Restricted registration - show details
Number of participants limited to 5.

The enrolment is done by the D-BIOL study administration.
W6 credits7GA. Wutz
AbstractThis course aims to provide students with a comprehensive overview of computational methods for sequence analysis and assist with developing skills for application of computational approaches by experimental scientists in the life sciences.
ObjectiveMethods for analyzing animal genomes are increasingly becoming important for applications in human health and biotechnology suggesting that the experience will be useful to develop relevant expertise for a broad range of functions. Students will have the opportunity to advance their knowledge in programming by focusing on algorithms for genome and gene sequence analysis. A major goal of the course will be to lead the student to an independent and empowered attitude towards computational problems. For reaching this goal the students will work on an implementation of a solution for a set real-world problem in genome and sequence analysis under guided supervision.
Content•Understanding the information in biological sequences and quantifying similarity
•Introduction to algorithms for sequence comparison and searches
•Implementation of sequence comparisons and searches in Python
•Accessing data formats associated with genome sequence analysis tasks
•Understanding the anatomy of a real world sequence analysis project
•Applying tools for sequence alignment and estimating error rates
•Ability to implement a solution to a problem in sequence analysis using Python
•Accessing genome annotation and retrieving relevant information in Pandas
•Application of Genomic intervals and arrays for sequence analysis with HTSeq

The course will consist of a series of lectures, assignments for implementing elementary tasks in Python, project development and discussion workshops, and 3 and a half week of practical work implementing a Pythons script as a solution to a real world problem associated with sequence analysis. At the end of the course students will explain their solutions and demonstrate the functionality of their implementations, which will then be discussed and commented on by the group. It is expected that students will be able to apply the knowledge to improve on concrete problems.
Prerequisites / Notice- It is recommended to bring your own computer with a Python installation to the course
- simple computers can be provided
- Programming basics with Python
Block Courses in 3rd Quarter of the Semester
From 7.11.2017 13:00 hr to 29.11.2017 17:00 hr
NumberTitleTypeECTSHoursLecturers
551-0355-00LPhytopathology Restricted registration - show details
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.
W6 credits7GM. Maurhofer Bringolf, B. McDonald
AbstractFundamentals (theoretical and practical) in phytopathology, eg. interaction between plants and plant-pathogenic microorganisms, morphology and lifecycles of plant-pathogenic fungi, evolution of plant-pathogenic fungi, biological control of plant diseases
ObjectiveFundamentals (theoretical and practical) in phytopathology, eg. interaction between plants and plant-pathogenic microorganisms, morphology and lifecycles of plant-pathogenic fungi, evolution of plant-pathogenic fungi, biological control of plant diseases

Insight into ongoing research projects
ContentPractical courses:

Experiments within ongoing phytopathological research projects
Macro- and microscopic diagnostic of plant diseases

Theoretical courses:

Fundamentals of phytopathology, eg. interaction between plants and plant-pathogenic microorganisms, morphology and lifecycles of plant-pathogenic fungi, evolution of plant-pathogenic fungi, biological control of plant diseases

Teaching language is english and german.
Lecture noteswill be distributed at the beginning of the course
529-0739-01LBiological Chemistry B: New Enzymes from Directed Evolution Experiments Restricted registration - show details
Number of participants limited to 12.
W6 credits7GP. A. Kast
AbstractDuring the block course in the fall semester, we will carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. The class with its very dense program consists of the practical course itself and an integrated series of seminar/lecture sessions.
ObjectiveAll technologies used for the experiments will be explained to the students in theory and in practice with the goal that they will be able to independently apply them for the course project and in future research endeavors. After the course, an individual report about the results obtained has to be prepared.
ContentThe class deals with a specifically designed and genuine research project. We intend to carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. By working in parallel, teams of 2 participants each will generate a variety of different variants of a chorismate mutase. Individual enzyme catalysts will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters, their molecular mass, and the integrity of the protein structure. The results obtained from the individual evolution experiments will be compared and discussed at the end of the class in a final seminar. We expect that during this lab course we will not only generate novel enzymes, but also gain new mechanistic insights into the investigated catalyst.
Lecture notesA script will be distributed to the participants on the first day of the course.
LiteratureGeneral literature to "Directed Evolution" and chorismate mutases, e.g.:

– Taylor, S. V., P. Kast & D. Hilvert. 2001. Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. 40: 3310-3335.

– Jäckel, C., P. Kast & D. Hilvert. 2008. Protein design by directed evolution. Annu. Rev. Biophys. 37: 153-173.

– Roderer, K. & P. Kast. 2009. Evolutionary cycles for pericyclic reactions – Or why we keep mutating mutases. Chimia 63: 313-317.

Further literature will be indicated in the distributed script.
Prerequisites / NoticeThis laboratory course will involve experiments that require a tight schedule and, particularly in the second half, very long (!) working days. The maximum number of participants for the laboratory class is limited, but surplus applicants may contact P. Kast directly to have their names added to a waiting list. A valid registration is considered a commitment for attendance of the entire course, as involved material orders and experimental preparations are necessary and, once the class has started, the flow of the experiments must not be interrupted by individual absences. In case of an emergency, please immediately notify P. Kast. For more information see Link, from where you can also download a flyer.
551-0336-00LMethods in Cellular Biochemistry Information Restricted registration - show details
Number of participants limited to 18.

The enrolment is done by the D-BIOL study administration.
W6 credits7GP. Picotti, J. Fernandes de Matos, U. Kutay, M. Peter, K. Weis
AbstractStudents will learn about biochemical approaches to analyze cellular functions. The course consists of practical projects in small groups, lectures and literature discussions. The course concludes with the presentation of results at a poster session.
ObjectiveStudents will learn to design, carry out and assess experiments using current biochemical and cell biological strategies to analyze cellular functions in a wide range of model systems. In particular they will learn novel imaging techniques along with biochemical approaches to understand fundamental cellular pathways. Furthermore, they will learn to assess strengths and limitations of the different approaches and be able to discuss their validity for the analysis of cellular functions.
LiteratureDocumentation and recommended literature (review articles and selected primary literature) will be provided during the course.
Prerequisites / NoticeThis course will be taught in English.
551-1515-00LInsulin Signaling Information Restricted registration - show details
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.
W6 credits7GM. Stoffel
AbstractIntroduction to the physiological and biochemical action of insulin signaling and its role in the fasted/feeding response and in obesity and diabetes.
ObjectiveThe students will obtain an overview about the current topics of research in insulin signaling and how it impacts on growth, metabolism and cell differentiation. They will learn to design experiments and use techniques necessary to analyze different aspects of insulin signaling,including physiological actions in whole animals as well as in tissue culture. Through lectures and literature seminars, they will learn about the open questions of insulin signaling research and discuss approaches to address these questions experimentally.

In practical lab projects the students will perform physiological in vivo studies as well as biochemical experiments. Finally, they will learn how to present and discuss their data. Student assessment is a graded semester performance based on individual performance in the laboratory, a written exam and the lab data presentation.
752-4020-00LExperimental Food Microbiology for Biologists Restricted registration - show details
Number of participants limited to 20.

Prerequisites: It is recommended to attend the course Lebensmittel-Mikrobiologie (752-4005-00L) as a preparation.
W6 credits7GM. Schuppler, M. Loessner, M. Schmelcher
AbstractTeaching of basic experimental knowledge for detection and identification of microorganisms in food. Practical experiments were accompanied by theoretical introductions. Students become acquainted with classical and state-of-the-art molecular techniques for the rapid detection of food borne pathogens and experiments in dependence on current research topics of the Laboratory of Food Microbiology.
ObjectiveIntroduction of methods and techniques of food microbiology
ContentTeaching of basic experimental knowledge for detection and identification of foodborne pathogens by applying state-of-the-art techniques as well as modern molecular techniques for the rapid identification of relevant foodborne pathogens.
Lecture notesHandouts were provided at the start of the course
Literature- Krämer: "Lebensmittel-Mikrobiologie" (Ulmer; UTB)
- Süssmuth et al.: "Mikrobiologisch-Biochemisches Praktikum" (Thieme)
Prerequisites / NoticeImportant information!
During the course we will work with the food-borne pathogen Listeria monocytogenes. Listeria monocytogenes represents a particular threat to pregnant women. Due to biosafety reasons participation is not allowed in case of pregnancy.
551-0363-00LComplex Carbohydrates - Biosynthesis, Structure & Function Information Restricted registration - show details
Number of participants limited to minimum 2 and maximum 8.

The enrolment is done by the D-BIOL study administration.
W6 credits7GM. Aebi, T. Keys
AbstractIn vitro & in vivo experiments will introduce current research on the biosynthesis, structure & function of protein-bound glycans in different pro- and eukaryotic microorganisms.
ObjectiveParticipants are familiar with the biosynthesis, structure and function of N-glycans in microorganisms and with the methods for their analysis.
Content* Topics: biosynthesis of asparagine-linked glycans in pro- and eukaryotes; structure of glycans in different organisms; methods to analyse the structure of glcans; function of glycans in protein quality control
* Introductory lectures
* Seminar with presentation and discussion of recent publications
* Experiments that exemplify the current research done in the group
551-0117-00LPlant Volatiles in Plant Insect Interactions Restricted registration - show details
Does not take place this semester.
Number of participants limited to 16.

The enrolment is done by the D-BIOL study administration.
W6 credits7GC. De Moraes
AbstractDuring the course students will become familiar with methods for the collection and analysis of plant-derived volatile organic compounds and explore the role of these compounds in mediating plant-insect interactions.
ObjectiveThe course will cover six main topics that will be connected throughout the experimental phase:
1) Plant volatile biosynthesis and classification
2) Insect olfactory physiology
3) Volatile-mediated plant-herbivore interactions
4) Volatile-mediated multitrophic interactions
5) Manipulation of plant volatile emission by vector- borne disease agents
6) Methods for volatile collection and analysis
The lab practical will be performed in a system consisting of the cabbage butterfly Pieris brasicae, its host plant Brassica oleracea (Brussels sprouts), and the parasitoid wasp Cotesia glomerata (natural enemy of P. brasicae).
Students will collect volatiles from herbivore-damaged and undamaged plants and learn how to identify and quantify these compounds through gas chromatography coupled with mass spectrometry and flame ionization detection (GG-MS-FID). Afterwards, they will be able to compare volatile emissions from herbivore-damaged and undamaged plants and identify important volatile compounds associated with herbivory. Finally, students will evaluate the effect of herbivore-induced volatile compounds on the behavior of the herbivore (P. brassicae) and its natural enemy (C. glomerata), using different behavioral assays, inculding Y-tube olfactometers and wind tunnels.
Lecture notesNo script
LiteratureThe recommended literature, including reviews and primary research articles, will be provided during the course.
Block Courses in 4th Quarter of the Semester
From 30.11.2017 08:00 hr to 22.12.2017 17:00 hr
NumberTitleTypeECTSHoursLecturers
551-0361-00LBiology of Bryophytes and Ferns Restricted registration - show details
Number of participants limited to 20.

The enrolment is done by the D-BIOL study administration.
W6 credits7GR. Holderegger, A. L. Bergamini
AbstractBryophytes: basic knowledge on the morphology, ecology, biogeography and endangerment of byrophytes; knowledge of common species; skills in the determination of bryophytes; field trip.
Ferns: basic knowledge on the life cycle, evolution and ecology of ferns; identification of Swiss fern species; field trips.
ObjectiveBryophytes: basic knowledge on the morphology, ecology, biogeography and endangerment of byrophytes; knowledge of common species; skills in the determination of bryophytes.
Ferns: basic knowledge on the life cycle, evolution and ecology of ferns; identification of Swiss fern species.
ContentBryophytes: Systematics and morphology of hornworts, liverworts and mosses and special themes such as ecology, biogeography, diversity and endangerment of bryophytes; one full-day field trip.
Ferns: Life cycle; evolutionary groups of ferns, lycopods and horsetails; breeding systems, micro- and macroevolution; ecology; full-day and half-day field trips.
Lecture notesHand-outs are available.
LiteratureVanderpoorten A. and Goffinet B. 2009. Introduction to Bryophytes. Cambridge University Press, Cambridge (non- mandatory).
Prerequisites / NoticeStudents have to present a poster on a special theme.

Grade according to poster presentation and contributions during the course.

Requirements: First and second year courses in Botany and Evolution.
551-1309-00LRNA-Biology Restricted registration - show details
Number of participants limited to 20.

The enrolment is done by the D-BIOL study administration.
W6 credits7GB. Mateescu, F. Allain, C. Beyer, J. Hall, R. Santoro, O. Voinnet, K. Weis, A. Wutz
AbstractIntroduction to the diversity of current RNA-research at all levels from structural biology to systems biology using mainly model systems like S. cerevisiae (yeast), mammalian cells.
ObjectiveThe students will obtain an overview about the diversity of current RNA-research. They will learn to design experiments and use techniques necessary to analyze different aspects of RNA biology. Through lectures and literature seminars, they will learn about the burning questions of RNA research and discuss approaches to address these questions experimentally. In practical lab projects the students will work in one of the participating laboratories. Finally, they will learn how to present and discuss their data in an appropriate manner. Student assessment is a graded semester performance based on individual performance in the laboratory, the written exam and the poster presentation.
LiteratureDocumentation and recommended literature will be provided at the beginning and during the course.
Prerequisites / NoticeThe course will be taught in English.
551-1511-00LParallels Between Tissue Repair and Cancer Information Restricted registration - show details
Number of participants limited to 15.

The enrolment is done by the D-BIOL study administration.
W6 credits7GS. Werner, U. Auf dem Keller, M. Bordoli, M. Schäfer
AbstractThis course aims at the understanding of the cellular and molecular mechanisms underlying tissue repair processes in response to different insults. The focus will be on repair of the skin and the liver. In addition, we will highlight the parallels and differences between tissue repair and cancer.
ObjectiveTo learn the cellular and molecular principles underlying tissue repair processes, in particular in the skin and in the liver, and the parallels and differences to cancer. To learn modern technologies in Molecular and Cellular Biology as well as Histology and to use these technologies to study questions related to mechanisms underlying tissue repair and cancer.
ContentThis course aims at the understanding of the cellular and molecular mechanisms underlying tissue repair processes in response to different insults. The focus will be on repair of the skin and the liver. In addition, we will highlight the parallels and differences between tissue repair and cancer. Experimental approaches include biochemical studies, molecular and cellular studies using cultured cell lines and primary cells, as well as analysis of murine and human tissues.
The course combines practical work with lectures, discussions, project preparations and presentations.
Lecture notessiehe Lernmaterialien
551-0371-00LGrowth Control: Insights from Yeast and Flies Information Restricted registration - show details
Number of participants limited to 8.

The enrolment is done by the D-BIOL study administration.
W6 credits7GH. Stocker, R. C. Dechant, M. Peter
AbstractAll organisms have to control their growth in accordance with environmental conditions. This course focuses on the analysis of growth regulation in the model organisms yeast and Drosophila. The participants will perform experiments in small teams to study insulin/TOR signaling as a key regulator of cellular growth. A particular focus will be the discussion of current research.
ObjectiveThe aims of the block course are that participants

(I) understand the function and evolution of insulin/TOR signaling

(II) learn how genetic approaches in different organisms contribute to the understanding of human diseases such as cancer

(III) will get familiarized with reading and discussing research articles

(IV) get a first exposure to current research.
ContentThe block course consists of

(I) experiments:

Teams of two students each will join research labs to work on current projects focusing on growth regulation in both single-cell eukaryotes (yeast) and multicellular animals (Drosophila). The students will present their projects and results to their colleagues.

(II) lectures on growth regulation in yeast and Drosophila.

(III) journal clubs to discuss recent literature.
Lecture notesLecture handouts
LiteratureOriginal research articles will be discussed during the course.
551-1403-00LImaging Bacterial Cells in a Native State by Electron Cryotomography Restricted registration - show details
Number of participants limited to 3.

The enrolment is done by the D-BIOL study administration.
W6 credits7GM. Pilhofer
AbstractThe goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures
ObjectiveStudents will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization.
Link
551-1417-00LIn Vivo Cryo-EM Analysis of Dynein Motor Proteins Restricted registration - show details
Number of participants limited to 3.

The enrolment is done by the D-BIOL study administration.
W6 credits7GT. Ishikawa
AbstractMotor proteins convert chemical energy into mechanical motion. In this block course, we study dynein motor proteins in cilia. Dynein causes conformational change upon ATP hydrolysis and finally generate ciliary bending motion. Participants will analyze cryo-EM data of cilia and visualize in vivo 3D structure of dynein to learn how motor proteins function in the cell.
ObjectiveThe goal of this course is to be familiar with structural biology techniques of cryo-electron tomography and single particle cryo-EM studies on motor proteins. The main focus is 3D image analysis of cryo-EM datasets acquired by highest-end microscopes. Participants will learn structure-function relationship at various scales: how the conformational change of motor proteins causes mechanical force and generates cellular motility.
ContentMotor proteins, such as dynein, myosin and kinesin, hydrolyze ATP to ADP and phosphate to convert chemical energy to mechanical motion. Their function is essential for intracellular transport, muscle contraction and other cellular motility as well as cell division. Motor proteins have been major targets of biophysical studies. There exist questions from atomic to tissue levels – how ATP hydrolysis causes conformational change of motor proteins; how their motion is regulated by calcium, phosphorylation and other factors; how motions of multiple motor proteins are coordinated to generate cellular motility. Structural biology has been playing central roles to answer these questions. X-ray crystallography and single particle cryo-EM address structural analysis at atomic resolution and try to reveal molecular mechanism of conformational change. Cryo-electron tomography analyze localization and 3D structure of motor proteins in the cell to explain how motions of molecular motors happen in the context of cellular environment and are integrated into cellular motion.
In this course, we study dyneins in cilia. Cilia are force-generating organelles, made by nine microtubules and thousands of dyneins. Dynein hydrolyzes ATP and undergoes conformational change, generating linear motion with respect to the microtubule. As a whole system, cilia integrate motions of these dyneins and orchestrate beating motion. To explain ciliary motion at molecular level, we need to know dynein conformational change in the cellular context. Cryo-electron tomography is recently developed technique to study molecular structures in vivo and therefore a suitable method to study dynein in cilia. Recently spatial resolution of these cryo-EM techniques was dramatically improved, driven by development of new types of detectors and electron optics.
The participants of this course will learn a program to analyze cryo-electron tomography and single particle cryo-EM data, acquired by highest-end electron microscopes and detectors in ETH and other places, and reconstruct 3D structure (tomogram) of cilia from various organisms (from green algae to human). They will further learn a program to study molecular structures from these tomograms (called subtomogram averaging) and apply it to reconstruct high-resolution 3D structure of dyneins, microtubules and regulatory proteins. This practical course is therefore mainly computational, but we will also provide students a chance of cilia preparation from green algae, cryo-EM data collection using an electron microscope in PSI and site-visit of highest-end electron microscope facility in ETH.
Lecture notesScripts will be distributed during the course.
LiteratureAn overview is given in the following review articles. Further literature will be indicated during the course.
Ishikawa (2017) “Axoneme structure from motile cilia” Cold Spring Harb. Perspect Biol. 9. doi: 10.1101/cshperspect.a028076.
Ishikawa (2017) “Cryo-electron tomography of motile cilia and flagella” Cilia 4, 3. doi: 10.1186/s13630-014-0012-7.
Block Courses in the 1st Half of the Semester
From 19.9.2017 13:00 hr to 3.11.2017 17:00 Uhr.
NumberTitleTypeECTSHoursLecturers
701-2437-01LLimnoecology (incl. two Practical Courses)
The course "701-2437-01L Limnoökologie" must be booked as "BIO 309 Limnoecology" in the block course tool.
W12 credits3V + 6U + 4PJ. Jokela, P. Spaak, F. Altermatt, K. J. Räsänen, C. T. Robinson
AbstractThis course combines Limnology (the study of inland waters in its broad sense) with ecological and evolutionary concepts. It deals with rivers, groundwater and lakes.
This course contains a lecture part, an experimental part, two determination courses (aquatic invertebrates and algae) as well as excursions.
ObjectiveDuring this course you will get an overview of the world's typical continental aquatic ecosystems. After this course you will be able to understand how aquatic organisms have adapted to their habitat, and how the interactions (e.g. food web) between organisms work.
During the experimental part of this course you will learn the principles of doing research to observe interrelations in aquatic ecosystems. You will measure and interpret biological and physical data (e.g. during experiments, field work) and present the collected knowledge.
After this course you will know the most important aquatic species groups (macroinvertebrates, microinvertebrates and cryptogames) in Switzerland and the most important identification traits.
ContentThe course contains a lecture part, an experimental part, two determination courses (aquatic invertebrates and algae) and field excursions.

Lecture:
The lecture part covers ecology and evolution of aquatic organisms in lentic and lotic waters. Topics include: Adaptations, distribution patterns, biotic interactions, and conceptual paradigms in freshwater ecosystems; important aspects regarding ecosystem metabolism and habitat properties of freshwaters; applied case studies and experiments testing ecological and evolutionary processes in freshwaters.

Practical part:
The practical part includes an excursion to Greifensee and a 3-day-excursion to the river Glatt in Niederuzwil, where you independently perform small research projects.
Additionally, you will perform in small groups an independent experiment in a research group at Eawag.

The taxonomic part will cover macroinvertebrates (e.g. Crustacean, aquatic insects), microinvertebrates and algae. The goal is to get to know the most common aquatic taxa in Switzerland, to identify them with commonly used identification literature, and to get an idea how these organisms are used in research and practice. (language: German, translation of the most important things during the course possible)
Lecture notesCourse notes and power point presentations provided during the course.
Prerequisites / NoticeThe maximal participating number of biology students is 14.

The course includes a 3-day-excursion to the river Glatt in Niederuzwil from 4th to 6th of October 2017.
Block Courses during Semester Break
NumberTitleTypeECTSHoursLecturers
551-0438-00LProtein Folding, Assembly and Degradation Restricted registration - show details
Number of participants limited to 14.

The enrolment is done by the D-BIOL study administration.
W6 credits7GR. Glockshuber, E. Weber-Ban
AbstractStudents will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese.
ObjectiveThe course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner.
ContentThe students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work.

Participation in one of the following projects will be possible:

Projects of the Glockshuber group:
- Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria.
- Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits.
- Identification of intermediates in the aggregation of the human Abeta peptide

Experimental work on these projects involves
- Molecular cloning, recombinant protein production in E. coli and protein purification
- Protein crystallization
- Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy
- Analysis of rapid reactions by stopped-flow fluorescence
- Negative-stain electron microscopy
- Light scattering



Projects of the Weber-Ban group:

- Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering
Lecture notesNo script
LiteratureLiterature related to the individual projects will be provided on the first day of the course.
Prerequisites / NoticeAttendance of the concept course "Biomolecular Structure and Mechanism I: Protein Structure and Function" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course.
551-1709-00LGenomic and Genetic Methods in Cell and Developmental Biology Restricted registration - show details
Number of participants limited to 11.

The enrolment is done by the D-BIOL study administration.
W6 credits7GA. Wutz, C. Beyer, M. Kopf, T. Schroeder, G. Schwank
AbstractThis course aims to provide students with a comprehensive overview of mammalian developmental biology and stem cell systems both on the theoretical as well as the experimental level. Centering the course on genetic and genomic methods engages the students in contemporary research and prepares for future studies in the course of semester and master projects.
Objective- Understanding mammalian development
- Introduction to stem cells systems
- Working with cultured cells
- Translational aspects of mammalian cell biology
ContentThe course will consist of a series of lectures, assay assignments, project development and discussion workshops, and 2 and a half week of lab work with different mammalian cell systems embedded in real life research projects. At the end of the course students will take an exam consisting of questions on the topic of the lectures and workshops. It is expected that students will be able to apply the knowledge to concrete problems.
  • First page Previous page Page  2  of  2     All