Suchergebnis: Katalogdaten im Frühjahrssemester 2021

Biologie Bachelor Information
Bachelor-Studium (Studienreglement 2013)
3. Studienjahr, 6. Semester
Konzeptkurse
NummerTitelTypECTSUmfangDozierende
529-0732-00LProteins and Lipids
Hinweis für BSc Biologiestudierende: Nur einer der beiden Konzeptkurse 529-0731-00 Nucleic Acids and Carbohydrates (Herbstsemester) oder 529-0732-00 Proteins and Lipids (Frühlingsemester) kann für das Bachelorstudium angerechnet werden.
W6 KP3GD. Hilvert
KurzbeschreibungAn overview of the relationship between protein sequence, conformation and function.
LernzielOverview of the relationship between protein sequence, conformation and function.
InhaltProteins, structures and properties, (bio)synthesis of polypeptides, protein folding and design, protein engineering, chemical modification of proteins, proteomics.
LiteraturGeneral Literature:
- T.E. Creighton: Proteins: Structures and Molecular Properties, 2nd Edition, H.W. Freeman and Company, New York, 1993.
- C. Branden, J. Tooze , Introduction to Protein Structure, Garland Publishing, New York, 1991.
- J. M. Berg, J. L. Tymoczko, L. Stryer: Biochemistry, 5th edition, H.W. Freeman and Company, New York, 2002.
- G.A. Petsko, D. Ringe: Protein Structure and Function, New Science Press Ltd., London, 2004.

Original Literature:
Citations from the original literature relevant to the individual lectures will be assigned weekly.
551-0324-00LSystems Biology Information W6 KP4VP. Picotti, M. Claassen, U. Sauer, B. Snijder, B. Wollscheid
KurzbeschreibungIntroduction to experimental and computational methods of systems biology. By using baker’s yeast as a thread through the series, we focus on global methods for analysis of and interference with biological functions. Illustrative applications to other organisms will highlight medical and biotechnological aspects.
Lernziel- obtain an overview of global analytical methods
- obtain an overview of computational methods in systems biology
- understand the concepts of systems biology
InhaltOverview of global analytical methods (e.g. DNA arrays, proteomics, metabolomics, fluxes etc), global interference methods (siRNA, mutant libraries, synthetic lethality etc.) and imaging methods. Introduction to mass spectrometry and proteomics. Concepts of metabolism in microbes and higher cells. Systems biology of developmental processes. Concepts of mathematical modeling and applications of computational systems biology.
Skriptno script
LiteraturThe course is not taught by a particular book, but some books are suggested for further reading:

- Systems biology in Practice by Klipp, Herwig, Kowald, Wierling und Lehrach. Wiley-VCH 2005
551-0320-00LCellular Biochemistry (Part II)W3 KP2VY. Barral, R. Kroschewski, A. E. Smith
KurzbeschreibungThis course will focus on molecular mechanisms and concepts underlying cellular biochemistry, providing advanced insights into the structural and functional details of individual cell components, and the complex regulation of their interactions. Particular emphasis will be on the spatial and temporal integration of different molecules and signaling pathways into global cellular processes.
LernzielThe full-year course (551-0319-00 & 551-0320-00) focuses on the molecular mechanisms and concepts underlying the biochemistry of cellular physiology, investigating how these processes are integrated to carry out highly coordinated cellular functions. The molecular characterization of complex cellular functions requires a combination of approaches such as biochemistry, but also cell biology and genetics. This course is therefore the occasion to discuss these techniques and their integration in modern cellular biochemistry.
The students will be able to describe the structural and functional details of individual cell components, and the spatial and temporal regulation of their interactions. In particular, they will learn to explain how different molecules and signaling pathways can be integrated during complex and highly dynamic cellular processes such as intracellular transport, cytoskeletal rearrangements, cell motility, and cell division. In addition, they will be able to illustrate the relevance of particular signaling pathways for cellular pathologies such as cancer or during cellular infection.
InhaltSpatial and temporal integration of different molecules and signaling pathways into global cellular processes, such as cell division, cell infection and cell motility. Emphasis is also put on the understanding of pathologies associated with defective cell physiology, such as cancer or during cellular infection.
LiteraturRecommended supplementary literature may be provided during the course.
Voraussetzungen / BesonderesTo attend this course the students must have a solid basic knowledge in chemistry, biochemistry, cell biology and general biology. Biology students have in general already attended the first part of the "Cellular Biochemistry" concept course (551-0319-00). The course will be taught in English.
In addition, the course will be based on a blended-learning scenario, where frontal lectures will be complemented with carefully chosen web-based teaching elements that students access through the ETH Moodle platform.
551-0314-00LMicrobiology (Part II)W3 KP2VW.‑D. Hardt, L. Eberl, J. Piel, J. Vorholt-Zambelli
KurzbeschreibungAdvanced lecture class providing a broad overview on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
LernzielThis concept class will be based on common concepts and introduce to the enormous diversity among bacteria and archaea. It will cover the current research on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
InhaltAdvanced class covering the state of the research in bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
SkriptUpdated handouts will be provided during the class.
LiteraturCurrent literature references will be provided during the lectures.
Voraussetzungen / BesonderesEnglish
551-0326-00LCell Biology Information W6 KP4VS. Werner, H. Gehart, W. Kovacs, M. Schäfer, U. Suter, A. Wutz, weitere Dozierende
KurzbeschreibungThis Course introduces principle concepts, techniques, and experimental strategies used in modern Cell Biology. Major topics include: neuron-glia interactions in health and disease; mitochondrial dynamics; stem cell biology; growth factor action in development, tissue repair and disease; cell metabolism, in particular sensing and signaling mechanisms, cell organelles, and lipid metabolism.
Lernziel-To prepare the students for successful and efficient lab work by learning how to ask the right questions and to use the appropriate techniques in a research project.
-To convey knowledge about neuron-glia interactions in health and disease.
- To provide information on different types of stem cells and their function in health and disease
-To provide information on growth factor signaling in development, repair and disease and on the use of growth factors or their receptors as drug targets for major human diseases
-To convey knowledge on the mechanisms underlying repair of injured tissues
-To provide the students with an overview of mitochondrial dynamics.
-Providing an understanding of RNA processing reactions and their regulations.
-To provide a comprehensive understanding of metabolic sensing mechanisms occurring in different cell types and organelles in response to glucose, hormones, oxygen, nutrients as well as lipids, and to discuss downstream signaling pathways and cellular responses.
-To provide models explaining how disturbances in complex metabolic control networks and bioenergetics can lead to disease and to highlight latest experimental approaches to uncover the intricacies of metabolic control at the cellular and organismal level.
-Providing the background and context that foster cross-disciplinary scientific thinking.
551-0318-00LImmunology IIW3 KP2VA. Oxenius, M. Kopf, S. R. Leibundgut, E. Slack, weitere Dozierende
KurzbeschreibungEinführung in die zellulären und molekularen Grundlagen des Immunsystems und die Immunreaktionen gegen verschiedene Pathogene, Tumore, Transplantate, und körpereigene Strukturen (Autoimmunität)
LernzielDie Vorlesung soll ein grundlegendes Verständnis vermitteln über:
- die Interaktion der verschiedenen Immunzellen auf zellulärer und molekularer Ebene?
- Erkennung und Abwehr ausgewählter Viren, Bakterien, und Parasiten.
- Abwehr von Tumoren.
- Mechanismen der Toleranz für körpereigene Moleküle.
- Funktion des Immunsystems im Darm und warum kommensale Bakterien keine Immunantwort auslösen.
- Immunpathologie und entzündliche Erkrankungen.
InhaltZiel dieser Vorlesung ist das Verständnis:
> Wie Pathogene vom unspezifischen Immunystem erkannt werden
> Wie Pathogene vom Immunsystem bekämpft werden
> Immunantworten der Haut, Lung, und Darms
> Tumorimmunologie
> Migration von Immunzellen
> Toleranz und Autoimmunität
> das Gedächtnis von T Zellen
SkriptDie Vorlesungsunterlagen der Dozenten sind verfügbar in Moodle
LiteraturEmpfohlen: Kuby Immunology (Freeman)
376-0209-00LMolecular Disease MechanismsW6 KP4VC. Wolfrum, H. Gahlon, M. Kopf
KurzbeschreibungIn this course the mechanisms of disease development will be studied. Main topics will be:

1. Influence of environmental factors with an emphasis on inflammation and the immune response.
2. Mechanisms underlying disease progression in metabolic disorders, integrating genetic and environmental factors.
3. Mechanisms underlying disease progression in cancer, integrating genetic and environment
LernzielTo understand the mechanisms governing disease development with a special emphasis on genetic and environmental associated components
SkriptAll information can be found at:

Link

The enrollment key will be provided by email
551-0307-01LMolecular and Structural Biology II: Molecular Machines and Cellular Assemblies
D-BIOL students are obliged to take part I and part II as a two-semester course.
W3 KP2VN. Ban, F. Allain, S. Jonas, M. Pilhofer
KurzbeschreibungThis course on advanced topics in Molecular Biology and Biochemistry will cover the structure and function of cellular assemblies. General topics in basic biochemistry will be further developed with examples of the function of large cellular machines involved in DNA packaging, translation, virus architecture, RNA processing, cell-cell interactions, and the molecular basis of CRISPER systems.
LernzielStudents will gain a deep understanding of large cellular assemblies and the structure-function relationships governing their function in fundamental cellular processes. The lectures throughout the course will be complemented by exercises and discussions of original research examples to provide students with a deeper understanding of the subjects and to encourage active student participation.
InhaltAdvanced class covering the state of the research in structural molecular biology of basic cellular processes with emphasis on the function of large cellular assemblies.
SkriptUpdated handouts will be provided during the class.
LiteraturThe lecture will be based on the latest literature. Additional suggested
literature:
Branden, C., and J. Tooze, Introduction to Protein Structure, 2nd ed.
(1995). Garland, New York.
Blockkurse
Anmeldung zu Blockkursen muss zwingend über die website Link
Anmeldung möglich von 19.12.2020 bis 09.01.2021

Bitte die ETH Aufnahmekriterien für die Aufnahme von Studierenden der ETH in ETH Blockkurse auf der Blockkurs-Anmeldeseite unter "Zuteilung" beachten.
Blockkurse im 1. Semesterviertel
Von 23.02.2021 bis 17.03.2021
NummerTitelTypECTSUmfangDozierende
551-0342-00LMetabolomics Belegung eingeschränkt - Details anzeigen
Number of participants limited to 15.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PN. Zamboni, U. Sauer
KurzbeschreibungThe course covers all basic aspects of metabolome measurements, from sample sampling to mass spectrometry and data analysis. Participants work in groups and independently perform and interpret metabolomic experiments.
LernzielPerforming and reporting a metabolomic experiment, understanding pro and cons of mass spectrometry based metabolomics. Knowledge of workflows and tools to assist experiment interpretation, and metabolite identification.
InhaltBasics of metabolomics: workflows, sample preparation, targeted and untargeted mass spectrometry, instrumentation, separation techniques (GC, LC, CE), metabolite identification, data interpretation and integration, normalization, QCs, maintenance.

Soft skills to be trained: project planning, presentation, reporting, independent working style, team work.
551-0339-00LMolecular Mechanisms of Cell Dynamics Belegung eingeschränkt - Details anzeigen
Number of participants limited to 18.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PE. Dultz, Y. Barral, U. Kutay, M. Peter, K. Weis
KurzbeschreibungApplication of current experimental strategies to study the dynamics of complex and highly regulated cellular processes.
LernzielIn this course, students will
- learn what principles govern cellular dynamics and how these are regulated.
- learn to evaluate and to apply current strategies to study the dynamics of complex and highly regulated cellular processes
InhaltDuring this Block-Course, the students will learn to
(1) describe the important mechanisms and regulators of dynamic processes in cells,
(2) perform experimental techniques to quantify dynamic cellular processes,
(3) evaluate and compare experimental strategies and model systems,
(4) formulate and present scientific concepts in an oral presentation.

Topics discussed will include
- mobility in the cell (passive and active)
- compartmentalization (by membranes and via phase separation)
- examples of cell biological processes dependent on mobility and compartmentalization.

Students will work in small groups in individual labs on one research project (8 full days of practical work; every group of students will stay in the same lab during the entire course). The projects are close to the actual research carried out in the participating research groups, but with a clear connection to the subject of the course.
LiteraturDocumentation and recommended literature (review articles and selected primary literature) will be provided during the course.
Voraussetzungen / BesonderesThis course will be taught in english.
551-1516-00LNeuron-Glia Interactions and Myelination in Health and Disease Belegung eingeschränkt - Details anzeigen
Number of participants limited to 15.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PU. Suter
KurzbeschreibungThe course provides general basic insights and new perspectives in the development, plasticity and repair of the nervous system. The focus is on molecular, cellular and transgenic approaches, mainly with the mouse as model system.
LernzielThrough a combination of practical work with lectures, discussions, project preparations and presentations, the students learn basic principles of neural plasticity and repair in health and disease. The course is linked to ongoing research projects in the lab to provide the participants with insights into current experimental approaches and strategies.
551-0118-00LCell Biology of Plant-Fungus Interaction Belegung eingeschränkt - Details anzeigen
Number of participants limited to 5.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PC. Sánchez-Rodríguez
KurzbeschreibungThe course is a collaboration of the Plant Cell Biology groups of ETHZ and UZH. The students will learn key concepts related with the remarkable ability of plants to adapt to challenges provided by their environment (both biotic, such as pathogens, and abiotic, like nutrient deficiencies). A multidisciplinary approach including molecular genetics, cell biology, biochemistry and bioinformatics tool
LernzielIn this course, students will get cell biological and molecular genetics insights into the developmental plasticity of plants to adapt to their environmental conditions using the model plant Arabidopsis thaliana. With this aim, they will actively participate in ongoing research projects tutored by doctoral students.
InhaltStudents will be engaged in research projects aimed to understand the specialized mechanisms evolved by the plants to grow under challenging environments. In a lecture series, the theoretical background for the projects and their interrelationship is provided.
Students will design and perform experiments, evaluate experimental results, present their projects, and discuss recent publications to understand the relevance of their work in the context of the current state of plant development and stress response.
SkriptNo script
LiteraturThe recommended literature and list of individual reading assignments will be provided during the course
Voraussetzungen / BesonderesAll general lectures will be held at ETH Centrum (LFW building). Students will be divided into small groups to carry out experiments at ETH (Central; LFW) and UZH (Botanical Garden)
Blockkurse im 2. Semesterviertel
Von 18.03.2021 bis 16.04.2021
NummerTitelTypECTSUmfangDozierende
376-1346-00LStudy of Epigenetic Mechanisms in Mental Health Belegung eingeschränkt - Details anzeigen
Number of participants limited to 10.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected.
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged.
-Any additional rules for individual courses have to be respected.
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible.
W6 KP7PI. Mansuy
KurzbeschreibungThis block course is focused on the study of the epigenetic mechanisms that regulate complex brain functions and behavior. It provides an overview of molecular methods used in experimental mice or in human samples to investigate epigenetic processes that control genome activity and gene expression, and are associated with cognitive functions and behavioral responses.
LernzielThe purpose is to learn the principles of major methods in epigenetics that allow examine genome activity at the level of DNA, RNA or protein, in the context of complex brain functions.
Inhalt4 independent projects for 3 students each covering various aspects of epigenetic mechanisms. It will focus on state-of-the-art techniques to measure or manipulate gene expression and gene activity in the adult brain or in cell culture, and analyse the effects in vitro or in vivo using omics analyses, molecular and biochemical tools and behavioral testing.
SkriptProvided at the beginning of the practical.
551-0352-00LIntroduction to Mass Spectrometry-Based Proteomics Belegung eingeschränkt - Details anzeigen
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PL. Gillet, P. Picotti
KurzbeschreibungProtein-Analyse durch Massenspektrometrie
Die folgende Thematik wird abgedeckt: Grundlagen der biologischen Massenspektrometrie einschliesslich Instrumentation, Datenaufnahme und -bearbeitung; Anwendung zur Identifizierung und Charakterisierung von Proteinen; Probevorbereitung; Proteomic-Strategien einschliesslich quantitative Analysen.
LernzielProbenvorbereitung fuer die MS Analyse (Trypsin Verdau, C18 Aufreinigung)
Prinzipien LC-MS basierter Datenaquisition (QTOF und/oder Ion Trap Instrumenten)
Qualitative Proteom Analyse (Protein Identifizierung mit Hilfe von Mascot und/oder Sequest Software)
Quantitative Proteom Analyse (unmarkierte und Isotopen markierte Strategien)
Analyse und Auswertung von Datensätzen zur Detektion von hoch- bzw. runter-regulierten Proteinen
551-0434-00LNMR Spectroscopy in Biology Belegung eingeschränkt - Details anzeigen
Number of participants limited to 6.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PF. Allain, A. D. Gossert, K. Wüthrich
KurzbeschreibungIn this block course, students actively participate in ongoing research projects in the research groups of Profs. Allain, Wüthrich and Dr. Gossert. The students will be tutored in their experimental work by experienced postdoc students. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as exercises and literature work.
LernzielThe course provides first "hands on" insight into applications of NMR spectroscopy in biological sciences. The course should enable the students to understand the potential and limitations of NMR applied to biological problems.
InhaltThe topics include studies of proteins, RNA and protein-RNA interactions,

Participation in one of the following projects will be possible:
- NMR of RNA
- NMR of several protein-RNA complexes (hnRNPF, nPTB, SR proteins)
- NMR studies of protein-ligand interactions
- dynamics of protein-RNA complexes
- Segmental isotopic labeling to study multidomain proteins
- NMR Methods Development
SkriptNo script
LiteraturLists of individual reading assignments will be handed out.
529-0810-01LOrganische Chemie II (für D-BIOL) Belegung eingeschränkt - Details anzeigen
-Wo immer möglich müssen die Distanzregeln eingehalten werden.
-Alle Studierende müssen während des gesamten Kurses Masken tragen. Bitte Reserve-Masken bereithalten. Zugelassen sind Hygienemasken (IIR) oder Schutzmasken (FFP2) ohne Ventil. Community Masken (Stoffmasken) sind nicht erlaubt.
-Die Installation und Aktivierung der Schweizer Covid-App ist sehr zu empfehlen.
-Alle zusätzlichen Regeln für einzelne Kurse müssen eingehalten werden -Studierende, die COVID-19-Symptome aufweisen, dürfen die ETH-Gebäude nicht betreten und müssen den verantwortlichen Kursleiter informieren.
W12 KP4PC. Thilgen
KurzbeschreibungBearbeiten eines organisch-synthetischen Teilprojekts aus der aktuellen Forschung einer Gruppe des Laboratoriums für Organische Chemie unter der Anleitung von Doktorierenden.
LernzielErlernen von Planung und Durchführung anspruchsvoller Mehrstufensynthesen unter Einbezug moderner Methoden; vertieftes Verständnis organisch-chemischer Reaktionen durch Experimente; Entwickeln eines organisch-synthetischen Forschungsprojekts; akkurates Protokollieren, Verfassen eines Berichts im Stil einer Veröffentlichung und Präsentieren der Ergebnisse in Form eines Kurzvortrags.
InhaltBearbeiten eines organisch-synthetischen Teilprojekts aus der aktuellen Forschung einer Gruppe des Laboratoriums für Organische Chemie unter der Anleitung von Doktorierenden.
SkriptKein Skript.
LiteraturKeine Pflichtliteratur. Literatur wird von den betreuenden Doktorierenden angegeben bzw. zur Verfügung gestellt.
Voraussetzungen / BesonderesVoraussetzungen: bestandenes Praktikum Organische Chemie I (529-0229-00); bestandene Sessionsprüfung Organische Chemie I (529-0221-00 bzw. 529-1011-00) / Organische Chemie II (529-0222-00 bzw. 529-1012-00). Die Zahl der Teilnehmenden ist auf 12 beschränkt.
551-1147-00LBioactive Natural Products from Bacteria Belegung eingeschränkt - Details anzeigen
Number of participants limited to 7.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PJ. Piel
KurzbeschreibungLab course. In small groups projects of relevance to current research questions in the field of bacterial natural product biosynthesis are addressed.
LernzielIntroduction to relevant subjects of the secondary metabolism of bacteria. Training in practical work in a research laboratory. Scientific writing in form of a research report.
InhaltResearch project on bacteria that produce bioactive natural products (e.g., Streptomycetes, Cyanobacteria, uncultivated bacteria). The techniques used will depend on the project, e.g. PCR, cloning, natural product analysis, precursor feeding studies, enzyme expression and analysis.
Skriptnone.
LiteraturWill be provided for each of the projects at the beginning of the course.
551-1554-00LMultigene Expression in Mammalian Cells Belegung eingeschränkt - Details anzeigen
Number of participants limited to 5.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PP. Berger, G. Schertler
KurzbeschreibungGenetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.
LernzielStudents will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.
InhaltWe will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.
Skriptnone
551-0436-00LCryo-Electron Microscopic Studies of Ribosomal Complexes with Biomedically Important Viral Proteins Belegung eingeschränkt - Details anzeigen
Number of participants limited to 15.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PN. Ban, D. Böhringer, M. A. Leibundgut, T. Lenarcic
KurzbeschreibungMany viruses have evolved specialized mechanisms to hijack the host gene expression machinery and employ cellular resources to regulate viral life cycle. They accomplish this through producing non-structural proteins that can, among other things, inhibit host protein synthesis. Participants of this course will visualize ribosomes in complex with a non-structural viral protein at high resolution.
LernzielThe goal of the course is to acquire the most important techniques and methods for the purification and structural characterisation of macromolecular complexes by transmission electron microscopy. The emphasis of the course is on the special practical requirements for the application of these techniques on macromolecular structures in the MDa range.
InhaltProtein synthesis is a very energy intensive process that can consume over half the total metabolism of a cell. In eukaryotes, translation is therefore tightly regulated at the stage of initiation. Regulatory processes are much more complex at this step than in prokaryotes and a large number of RNA modification processes and translation initiation factors are required to ensure faithful initiation, elongation and termination of translation. However, several viruses may interfere with host translation by affecting the initiation step or by modifying the activity of key initiation factors to ensure an efficient translation of viral mRNA. Amongst such viruses is also SARS-CoV-2, which infects a large variety of vertebrate species. On entering host cells, the viral genomic RNA is translated by the cellular protein synthesis machinery to produce a set of non-structural proteins, which by inhibiting host translation render cell conditions favorable for viral production. Within the Ban lab, we have studied, and continue to investigate, medically relevant viral proteins. This course will involve producing and attempting to determine the structure of a non-structural viral protein in a ribosome-bound form.

A variety of purification techniques, including affinity chromatography and ultracentrifugation, will be used during the purification of macromolecular complexes. Purified assemblies will be then investigated functionally. Students will then characterise their samples structurally through transmission electron cryo-microscopy (cryo-EM), including sample preparation, microscopy, data evaluation and the calculation of densities. Finally, students will learn how to build and refine molecular models into parts of the calculated cryo-EM density. The participants will be working on a closed project related to current research within the laboratory and throughout the course the practical work will be accompanied by brief theoretical introductions. The principal aim of the course is to strengthen the skills required to independently conduct meaningful biophysical and biochemical experiments and to provide an early introduction into the structural characterisation of cellular macromolecular assemblies.
SkriptA script will be distributed at the beginning of the course that will cover the experiments to be performed, provide references to the relevant literature and suggest points for further consideration for interested students.
LiteraturLiterature
A basic overview is provided within the references below. Further reading and citations shall be detailed in the course script.
- A. Fersht, Structure and mechanism in protein science, Freeman, 1999 (Chapters 1 and 6).
- M. van Heel et al., Single-particle electron cryo microscopy: towards atomic resolution, Quart. Rev. Biophys. (33), 307-369 (2000).
Voraussetzungen / BesonderesThe course will be held in English. Students should have either completed courses:
551-0307-00L Biomolecular Structure and Mechanism I: Protein Structure and Function
551-0307-01L Biomolecular Structure and Mechanism II: Large Cellular Machines
or equivalent courses covering the structure and function of biological macromolecules.
Blockkurse im 3. Semesterviertel
Von 20.04.2021 bis 12.05.2021
NummerTitelTypECTSUmfangDozierende
551-0362-00LMolecular Health: Biomedical Analysis of the Extracellular Interactome Belegung eingeschränkt - Details anzeigen
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
W6 KP7PB. Wollscheid, E. Tschudy-Milani
KurzbeschreibungIn this course you will learn to measure, integrate, analyze and validate the cellular surfaceome as a complex information gateway connecting the intracellular to the extracellular interactome. You will apply next generation technologies at the interface of biology, chemistry, medicine and bioinformatics to establish the surfaceome proteotype and its signaling interaction networks.
Lernziel"If a cell surface molecule such as the B cell receptor would have the size of a human being, then the cell surface of a B cell would have roughly the size of three times NYC Central Park." How many people/proteins/proteoforms reside in this space ("Surfaceome")? Similar to humans, proteins don't act alone. Function is encoded in dynamic protein-protein interactions. How are these proteoforms organized in signaling islands/networks in order to fulfill specific cellular functions ("Interactome")? What are the ligands interacting with the surfaceome to communicate information from other cells & tissues in the body? What goes wrong in these signaling islands if we get sick?

In this course you will learn to measure, integrate, analyze and validate the cellular surfaceome and its signaling islands as a complex information gateway connecting the intracellular to the extracellular interactome. You will apply next generation technologies at the interface of biology, chemistry, medicine and bioinformatics to generate unprecedented data to establish the surfaceome proteotype and its signaling interaction networks. This digital proteotype data layer provides the basis for generating qualitative and quantitative surfaceome models explaining how molecular nanoscale organization influences cellular signaling and biological function.
Inhalt"If a cell surface molecule such as the B cell receptor would have the size of a human being, then the cell surface of a B cell would have roughly the size of three times NYC Central Park." How many people/proteins/proteoforms reside in this space ("Surfaceome")? Similar to humans, proteins don't act alone. Function is encoded in dynamic protein-protein interactions. How are these proteoforms organized in signaling islands/networks in order to fulfill specific cellular functions ("Interactome")? What are the ligands interacting with the surfaceome to communicate information from other cells & tissues in the body? What goes wrong in these signaling islands if we get sick?

In this course you will learn to measure, integrate, analyze and validate the cellular surfaceome and its signaling islands as a complex information gateway connecting the intracellular to the extracellular interactome. You will apply next generation technologies at the interface of biology, chemistry, medicine and bioinformatics to generate unprecedented data to establish the surfaceome proteotype and its signaling interaction networks. This digital proteotype data layer provides the basis for generating qualitative and quantitative surfaceome models explaining how molecular nanoscale organization influences cellular signaling and biological function.
LiteraturD. Bausch-Fluck, E. S. Milani, B. Wollscheid, Surfaceome nanoscale organization and extracellular interaction networks, Curr. Opin. Chem. Biol. 48, 26–33 (2019).

Link
Voraussetzungen / BesonderesThis course requires a basic knowledge in mass spectrometry based proteomics and experience in computational data processing using R or MatLab. Ideally this course should be combined with course 551-0352-00L "Introduction to Mass Spectrometry-based Proteomics".
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