Search result: Catalogue data in Autumn Semester 2019

Number	Title	Type	ECTS	Hours	Lecturers
Biology Bachelor
3. Year, 5. Semester
Block Courses Registration for Block courses is mandatory. Please register under https://www.uzh.ch/zoolmed/ssl-dir/Blockkurse_UNIETH.php. Registration period: from 22.7.2019 - 6.8.2019. Please note the ETH admission criteria for the admission of ETH students to ETH block courses on the block course registration website under "allocation".
Block Courses during Semester Break
551-0438-00L	Protein Folding, Assembly and Degradation Number of participants limited to 14. The enrolment is done by the D-BIOL study administration.	W	6 credits	7G	R. Glockshuber, E. Weber-Ban
Abstract	Students will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese.
Learning objective	The course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner.
Content	The students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work. Participation in one of the following projects will be possible: Projects of the Glockshuber group: - Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria. - Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits. - Identification of intermediates in the aggregation of the human Abeta peptide Experimental work on these projects involves - Molecular cloning, recombinant protein production in E. coli and protein purification - Protein crystallization - Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy - Analysis of rapid reactions by stopped-flow fluorescence - Negative-stain electron microscopy - Light scattering Projects of the Weber-Ban group: - Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering
Lecture notes	No script
Literature	Literature related to the individual projects will be provided on the first day of the course.
Prerequisites / Notice	Attendance of the concept course "Biomolecular Structure and Mechanism I: Protein Structure and Function" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course.
551-1709-00L	Genomic and Genetic Methods in Cell and Developmental Biology Number of participants limited to 8. The enrolment is done by the D-BIOL study administration.	W	6 credits	7G	A. Wutz, C. Beyer, M. Kopf, T. Schroeder
Abstract	This course aims to provide students with a comprehensive overview of mammalian developmental biology and stem cell systems both on the theoretical as well as the experimental level. Centering the course on genetic and genomic methods engages the students in contemporary research and prepares for future studies in the course of semester and master projects.
Learning objective	- Understanding mammalian development - Introduction to stem cells systems - Working with cultured cells - Translational aspects of mammalian cell biology
Content	The course will consist of a series of lectures, assay assignments, project development and discussion workshops, and 2 and a half week of lab work with different mammalian cell systems embedded in real life research projects. At the end of the course students will take an exam consisting of questions on the topic of the lectures and workshops. It is expected that students will be able to apply the knowledge to concrete problems.

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