Search result: Catalogue data in Spring Semester 2018

Number	Title	Type	ECTS	Hours	Lecturers
Biotechnology Master More information at www.master-biotech.ethz.ch
Master Studies (Programme Regulations 2017)
Practical Training Students need to acquire a total of 14 ECTS in lab courses. All listed lab courses are mandatory.
636-0207-00L	Lab Course: Cellular Engineering Stem Cells Only for Biotechnology MSc, Programme Regulations 2017. Attention: This lab course was offered in previous semesters with the number: 626-0806-00L "Laboratory Course Stem Cell Purification, Culture and Manipulation”. Students that already passed course 626-0806-00L cannot receive credits for course 636-0207-00L.	O	2 credits	6P	T. Schroeder
Abstract	Mammalian stem cells of different organs are purified, cultured, differentiated, analyzed and manipulated. Plasmids and viral vectors will be cloned, produced and transfected / transduced to manipulate stem cells. Computational and analytical molecular biology methods, FACS and imaging and lectures complement the program.
Objective	Independent planning and conducting of experiments with mammalian stem cells including all steps from culturing different cell lines to DNA transfection / transduction and expression analysis by different analytical methods. Documenting and writing a report on conducted experiments and results.
Content	Practical course on purification of primary mammalian stem cells, culture of primary stem cells and stem cell lines, characterization, manipulation and differentiation of stem cells. Construction of plasmids or viral vectors for gene expression, DNA transfer by transfection and transduction, analysis of gene expression by fluorescent proteins, PCR, fluorescence-activated cell sorting (FACS), imaging. Documentation of experiments in a laboratory journal, writing of a report on the experiments and results.
636-0206-00L	Lab Course: Cellular Engineering Mammalian Cells Only for Biotechnology MSc, Programme Regulations 2017 Attention: This lab course was offered in previous semesters with the number: 626-0802-00L "Practical Course in Mammalian Cell Biotechnology”. Students that already passed course 626-0802-00L cannot receive credits for course 636-0206-00L.	O	2 credits	6P	M. Fussenegger, M. Folcher
Abstract	Mammalian cells will be transfected and transduced for the production of biopharmaceuticals, for drug discovery as well as for the design of synthetic biology-inspired programmable gene circuits. A wide array of analytical techniques, lectures, and excursions to biotech companies will complement the practical part.
Objective	Independent planning and conducting of experiments with mammalian cells including all steps from culturing different cell lines to DNA transfection/transduction and expression analysis using a wide array of analytical methods.
Content	A practical course on characterization and cultivation of mammalian cells, DNA transfer by transfection, construction of synthetic gene networks, analysis of gene expression by enzymatic and immunological methods and fluorescent proteins, bioprocessing, mammalian cell-based assays for drug discovery and diagnostics. Excursions to Biotech/Pharma companies.
Lecture notes	Will be distributed on first day of the practical course
636-0204-00L	Lab Course: Microbial Biotechnology Only for Biotechnology MSc, Programme Regulations 2017.	O	2 credits	5P	M. Held
Abstract	Students will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
Objective	Students will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
Content	Block A: Handling and preparation and of microbial libraries D1: Introduction to microbiological cultures and monoseptic working techniques. D2: Plasmid-based expression systems and variation of XFP synthesis levels via site-directed RBS mutagenesis. Block B: Library screening D3: In vivo screening for XFP expression levels. D4: Analysis of XFP levels via SDS-PAGE analysis. RBS-sequencing. Block C: Hit recovery and validation D5: In silico analysis of RBS variants. D6: Cellular XFP content for selected variants at different culture conditions. Block D: Data analysis and presentation D7: Protein expression analysis. Q&A for reports and presentations. D8: Final presentations and wrap-up.
Lecture notes	Material will be provided during the course.
Literature	(1) Reetz MT, Kahakeaw D, and Lohmer R. "Addressing the numbers problem in directed evolution." ChemBioChem 2008 (2) Jeschek M, Gerngross D, and Panke S. “Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.” Nat. Commun. 2016 (3) Salis HM. “The ribosome binding site calculator.” Methods Enzymol. 2011 (4) Nienhaus G, Nienhaus K, and Wiedenmann J. "Structure–Function Relationships in Fluorescent Marker Proteins of the Green Fluorescent Protein Family." Fluorescent Proteins I. Springer Berlin Heidelberg, 2011 General introduction to microbiology: (5) Schlegel HG, and Zaborosch C. “General Microbiology.” Cambridge University Press 1993 (6) Pirt JS. “Principles of microbe and cell cultivation.” Blackwell Scientific Publications 1975
636-0205-00L	Lab Course: Mammalian Gene Circuits Only for Biotechnology MSc, Programme Regulations 2017.	O	2 credits	5P	Y. Benenson
Abstract	The students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level.
Objective	The objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization.
Content	The course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1. Week 2: Cloning of the constructs, purification and characterization of DNA constructs Week 3: Cell culture transfection, microscopy and flow cytometry characterization Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments.
Lecture notes	Preparatory materials will be provided before the start of the course.
Literature	Will be provided before the course

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