Suchergebnis: Katalogdaten im Frühjahrssemester 2019
Biologie Master | ||||||
Wahlvertiefungen | ||||||
Wahlvertiefung: Mikrobiologie und Immunologie | ||||||
Obligatorische Konzeptkurse | ||||||
Nummer | Titel | Typ | ECTS | Umfang | Dozierende | |
---|---|---|---|---|---|---|
551-0314-00L | Microbiology (Part II) | O | 3 KP | 2V | W.‑D. Hardt, L. Eberl, H.‑M. Fischer, J. Piel, J. Vorholt-Zambelli | |
Kurzbeschreibung | Advanced lecture class providing a broad overview on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||
Lernziel | This concept class will be based on common concepts and introduce to the enormous diversity among bacteria and archaea. It will cover the current research on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||
Inhalt | Advanced class covering the state of the research in bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||
Skript | Updated handouts will be provided during the class. | |||||
Literatur | Current literature references will be provided during the lectures. | |||||
Voraussetzungen / Besonderes | English | |||||
551-0318-00L | Immunology II | O | 3 KP | 2V | A. Oxenius, M. Kopf, S. R. Leibundgut, E. Slack, weitere Dozierende | |
Kurzbeschreibung | Einführung in die zellulären und molekularen Grundlagen des Immunsystems und die Immunreaktionen gegen verschiedene Pathogene, Tumore, Transplantate, und körpereigene Strukturen (Autoimmunität) | |||||
Lernziel | Die Vorlesung soll ein grundlegendes Verständnis vermitteln über: - die Interaktion der verschiedenen Immunzellen auf zellulärer und molekularer Ebene? - Erkennung und Abwehr ausgewählter Viren, Bakterien, und Parasiten. - Abwehr von Tumoren. - Mechanismen der Toleranz für körpereigene Moleküle. - Funktion des Immunsystems im Darm und warum kommensale Bakterien keine Immunantwort auslösen. - Immunpathologie und entzündliche Erkrankungen. | |||||
Inhalt | Ziel dieser Vorlesung ist das Verständnis: > Wie Pathogene vom unspezifischen Immunystem erkannt werden > Wie Pathogene vom Immunsystem bekämpft werden > Immunantworten der Haut, Lung, und Darms > Tumorimmunologie > Migration von Immunzellen > Toleranz und Autoimmunität > das Gedächtnis von T Zellen | |||||
Skript | Die Vorlesungsunterlagen der Dozenten sind verfügbar in Moodle | |||||
Literatur | Empfohlen: Kuby Immunology (Freeman) | |||||
Wahlpflicht Masterkurse | ||||||
Nummer | Titel | Typ | ECTS | Umfang | Dozierende | |
701-1310-00L | Environmental Microbiology | W | 3 KP | 2V | M. H. Schroth, M. Lever | |
Kurzbeschreibung | Microorganisms catalyze a large number of reactions that are of great importance to terrestrial and aquatic environments. To improve our understanding of the dynamics of a specific environment, it is important to gain a better understanding of microbial structures and their functions under varying environmental conditions. | |||||
Lernziel | Students will learn basic concepts in microbial ecology. Qualitative and quantitative concepts will be presented to assess microbial communities and associated processes in terrestrial and aquatic environments. Microbial diversity in such ecosystems will be illustrated in discussions of selected habitats. | |||||
Inhalt | Lectures will cover general concepts of environmental microbiology including (i) quantification of microbial processes, (ii) energy fluxes in microbial ecosystems, (iii) application of state-of-the-art microbiological and molecular tools, and (iv) use of isotope methods for identification of microbial structures and functions. Topics to illustrate the microbial diversity of terrestrial and aquatic ecosystems will include (i) interactions between microbes and mineral/metallic solid phases, (ii) microbial carbon and nutrient cycling, (iii) microbial processes involved in the turnover of greenhouse gases, (iv) biofilms and microbial mats, (v) bioremediation, (vi) microorganisms in extreme habitats, and (vii) microbial evolution and astrobiology. | |||||
Skript | available at time of lecture - will be distributed electronically as pdf's | |||||
Literatur | Brock Biology of Microorganisms, Madigan M. et al., Pearson, 14th ed., 2015 | |||||
551-1100-00L | Infectious Agents: From Molecular Biology to Disease Number of participants limited to 22. Requires application until 2 weeks before the start of the semester; selected applicants will be notified one week before the first week of lectures. (if you missed the deadline, please come to the first date to see, if there are any slots left) | W | 4 KP | 2S | W.‑D. Hardt, L. Eberl, U. F. Greber, A. B. Hehl, M. Kopf, S. R. Leibundgut, C. Münz, A. Oxenius, P. Sander | |
Kurzbeschreibung | Literature seminar for students at the masters level and PhD students. Introduction to the current research topics in infectious diseases; Introduction to key pathogens which are studied as model organisms in this field; Overview over key research groups in the field of infectious diseases in Zürich. | |||||
Lernziel | Working with the current research literature. Getting to know the key pathogens serving as model organisms and the research technologies currently used in infection biology. | |||||
Inhalt | for each model pathogen (or key technology): 1. introduction to the pathogen 2. Discussion of one current research paper. The paper will be provided by the respective supervisor. He/she will give advice (if required) and guide the respective literature discussion. | |||||
Skript | Teachers will provide the research papers to be discussed. Students will prepare handouts for the rest of the group for their assigned seminar. | |||||
Literatur | Teachers will provide the research papers to be discussed. | |||||
Voraussetzungen / Besonderes | Restricted to max 22 students. Please sign up until two weeks before the beginning of the semester via e-mail to Link and include the following information: 551-1100-00L; your name, your e-mail address, university/eth, students (specialization, semester), PhD students (research group, member of a PhD program? which program?). The 22 students admitted to this seminar will be selected and informed by e-mail in the week befor the beginning of the semester by W.-D. Hardt. The first seminar date will serve to form groups of students and assign a paper to each group. | |||||
551-1118-00L | Cutting Edge Topics: Immunology and Infection Biology II | W | 2 KP | 1S | A. Oxenius, B. Becher, C. Halin Winter, N. C. Joller, M. Kopf, S. R. Leibundgut, C. Münz, F. Sallusto, R. Spörri, M. van den Broek, Uni-Dozierende | |
Kurzbeschreibung | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. | |||||
Lernziel | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. Ziel der Veranstaltung ist die Konfrontation von Studenten und Doktoranden mit aktuellen Forschungsthemen und mit wissenschaftlicher Vortragsform. Studenten und Doktoranden wird die Gelegenheit geboten, sich mit diversen Themen vertieft auseinander zu setzen, welche oft in den Konzeptkursen nur knapp präsentiert werden und mit Experten auf dem Gebiet zu diskutieren. | |||||
Inhalt | Immunologie und Infektionsbiologie. Die speziellen Themen variieren jedes Semester und hängen von den eingeladenen Experten ab. | |||||
551-1104-00L | Ausgewählte Kapitel der Mykologie im Wald | W | 2 KP | 1V | I. L. Brunner, M. Peter Baltensweiler, D. H. Rigling | |
Kurzbeschreibung | Lebensweisen und Funktionen von symbiotischen, saproben und pathogenen Pilzen, Lebensgemeinschaften der Mykorrhiza und funktionelle Aspekte der Mykorrhizadiversität, Evolution und phylogenetische Aspekte der Pflanzen-Pilz Interaktionen, inter- und intraspezifische Myzelinteraktionen, Rolle der Pilze bei Nährstofferschliessung und Verwitterung. | |||||
Lernziel | Vertiefte Kenntnisse der Biologie und Ökologie der Pilze im Wald. Selbständige Auseinandersetzung mit aktueller Literatur. | |||||
Inhalt | Vertiefte Behandlung ausgewählter Themen der Pilze im Ökosystem Wald: Lebensweisen und Funktionen von symbiotischen, saproben und pathogenen Pilzen, Lebensgemeinschaften der Mykorrhiza und funktionelle Aspekte der Mykorrhizadiversität, Evolution und phylogenetische Aspekte der Pflanzen-Pilz Interaktionen, Inter- und intraspezifische Myzelinteraktionen, Rolle der Pilze bei Nährstofferschliessung und Verwitterung. Die Grundlagen werden in Vorlesungen vermittelt. Daneben selbständige Vertiefung des Stoffes mit Hilfe aktueller Literatur und Präsentationen. | |||||
Skript | Unterlagen zum Kurs werden abgegeben. | |||||
Literatur | Smith S.E. and Read D.J. 1997. Mycorrhizal Symbiosis. Academic Press, 2nd ed., pp. 605. | |||||
551-0216-00L | Mykologischer Feldkurs Maximale Teilnehmerzahl: 8 | W | 3 KP | 3.5P | A. Leuchtmann | |
Kurzbeschreibung | Exkursionen zum Sammeln von Pilzen und anschliessendes Studium der Funde im Kursraum. Hauptfokus sind Kleinpilze (Ascomyceten): sie erhalten einen Einblick in die Vielfalt der Formen und eine Einführung ins Bestimmen. Zudem wird auf die Ökologie und Funktion der Pilze in ausgewählten Habitaten eingegangen, sowie ausgewählte Beispiele von einheimischen Speise- und Giftpilzen gezeigt. | |||||
Lernziel | Erweiterung und Vertiefung der systematisch-taxonomischen Kenntnisse der Pilze, mit Fokus auf Ascomyceten. Teilnehmer kennen ökologische Funktionen der Pilze als Mutualisten, Saprobionten oder Parasiten von Pflanzen in verschiedenen Ökosystemen. | |||||
Inhalt | Einführung ins Reich der Pilze, Merkmale der Pilze und Überblick über deren systematische Gliederung. Exkursionen zum Sammeln von Ascomyceten in ausgewählten Lebensräumen. Kennenlernen von notwendigen Sammel- und Präparationstechniken, Einführung in die Ökologie und Funktion der Pilze, Untersuchung und Bestimmen von Pilzen mit optischen Hilfsmitteln im Kursraum, Einblick in Formenvielfalt ausgewählter Pilzgruppen (Ascomyecten), Beispiele von Gift- und Speisepilzen. | |||||
Skript | Kursunterlagen werden abgegeben | |||||
Literatur | Spezialliteratur für die Bestimmung der Familien, Gattungen und Arten der mitteleuropäischen Mykoflora. | |||||
Voraussetzungen / Besonderes | Der Kurs ist auf maximal 8 Teilnehmende beschränkt. Schriftliche Anmeldung erforderlich. Das Kursgeld von Fr. 180.- muss von den Kursteilnehmern übernommen werden. Vor dem Kurs (Freitag 23. Aug. 2019) findet eine halbtägige Einführung in Zürich statt, deren Besuch ist obligatorisch. | |||||
551-1132-00L | Allgemeine Virologie Findet dieses Semester nicht statt. | W | 2 KP | 1V | ||
Kurzbeschreibung | Einführung in die Grundlagen der Virologie, welche die Charakterisierung von Viren, die Interaktionen der Viren mit infizierten Zellen, Wirten und Populationen, die Grundlagen des Schutzes vor Infektion und die Virusdiagnostik beinhaltet. | |||||
Lernziel | Einführung in die Grundlagen der Virologie. | |||||
Inhalt | Grundlagen der Virologie. Charakterisierung von Viren. Virus-Zell-Interaktionen. Virus-Wirt-Interaktionen. Virus-Population-Interaktionen. Schutz vor Virusinfektion. Virusdiagnostik. | |||||
Skript | Die Vorlesung ist auf dem Lehrbuch "Allgemeine Virologie" von Kurt Tobler, Mathias Ackermann und Cornel Fraefel aufgebaut. Die Präsentationsfolien und ausgewählte Primärliteratur werden 24 bis 48 Stunden vor den Lektionen als .pdf-Dateien bereitgestellt. | |||||
Literatur | Kurt Tobler, Mathias Ackermann und Cornel Fraefel, Allgemeine Virologie, 2016, 1. Auflage UTB-Band-Nr.:4516 Haupt Verlag Bern ISBN: 978-3-8252-4516-0 | |||||
Voraussetzungen / Besonderes | Grundkenntnisse in Molekularbiologie, Zellbiologie und Immunologie | |||||
551-0140-00L | Epigenetics | W | 4 KP | 2V | A. Wutz, U. Grossniklaus, R. Paro, R. Santoro | |
Kurzbeschreibung | Epigenetik untersucht die Vererbung von Merkmalen, die nicht auf eine Veränderung der DNA Sequenz zurückgeführt werden kann. Die Vorlesung gibt einen Überblick über epigenetische Phänomene und erklärt die zugrundeliegenden molekularen Mechanismen. Die Rolle von epigenetischen Prozessen bei der Krebsentstehung und anderen Krankheiten wird diskutiert. | |||||
Lernziel | Das Ziel des Kurses ist das Verständnis von epigenetischen Mechanismen und deren Funktion in der Entwicklung von Organismen, bei Regenerationsprozessen oder bei der Entstehung von Krankheiten. | |||||
Inhalt | Themen - Historischer Überblick, Konzepte und Vergleich Genetik vs. Epigenetik - Biologie von Chromatin: Struktur und Funktion, Organisation im Kern und die Rolle von Histon Modifikationen bei Prozessen wie Transkription und Replikation. - DNA-Methylierung als epigenetische Modifikation - Weitergabe epigenetischer Modifikationen während der Zellteilung: das Zellgedächtnis - Stabilität/Revertierbarkeit epigenetischer Modifikationen: zelluläre Plastizität und Stammzellen. - Genomisches Imprinting in Pflanzen und in Säugern - X Chromosom Inaktivierung und Dosiskompensation - Positionseffekte, Paramutationen und Transvektion - RNA-induziertes Gensilencing - die Rolle von epigenetischen Prozessen bei der Krebsentstehung oder der Zellalterung. | |||||
751-4904-00L | Mikrobielle Schädlingsbekämpfung | W | 2 KP | 2G | J. Enkerli, G. Grabenweger, S. Kuske Pradal | |
Kurzbeschreibung | Die Vorlesung vermittelt konzeptionelle, sowie biologische und ökologische Grundlagen in mikrobieller Schädlingsbekämpfung. Anhand von Beispielen werden die Methoden und Techniken zur Entwicklung und Überwachung von mikrobiellen Schädlingsbekämpfungsmitteln erarbeitet. | |||||
Lernziel | Kennenlernen der wichtigsten Gruppen von insektenpathogenen Mikroorganismen und deren Eigenschaften. Vertraut werden mit den nötigen Schritten für die Entwicklung von Schädlingsbekämpfungsmitteln. Verstehen der Techniken und Methoden, die für das Überwachen von Feldapplikationen benützt werden, und Kennen der Registrierungsanforderungen für mikrobielle Schädlingsbekämpfungsmittel. | |||||
Inhalt | Die in der biologischen Schädlingsbekämpfung gebrauchten Definitionen und generell verwendete Ausdrücke werden erarbeitet. Ferner werden biologische und ökologische Aspekte aller Arthropoden-pathogenen Gruppen (Viren, Bakterien Pilze und Nematoden) und ihre Vor- und Nachteile in Bezug auf biologische Schädlingsbekämpfung diskutiert. Ein Schwergewicht wird dabei auf die Pilzgruppen Hypocreales und Entomophtorales gelegt. Anhand von Beispielen wird aufgezeigt, wie Projekte in biologischer Schädlingsbekämpfung aufgebaut werden können, wie Pathogene appliziert werden und wie die Effizienz, Effekte auf Nicht-Zielorganismen, Persistenz und Verbreitung überwacht werden. Im Weiteren werden die nötigen Schritte in der Entwicklung eines Produktes, kommerzielle Aspekte und die Registrierungsanforderungen besprochen. | |||||
Skript | Die grundlegenden Aspekte werden als Skript (Präsentationsunterlagen) abgegeben. | |||||
Literatur | Hinweise auf zusätzliche Literatur werde in der Lehrveranstaltung gegeben. | |||||
551-1126-00L | Technologies in Molecular Microbiology | W | 4 KP | 2V | H.‑M. Fischer, B. Christen, M. Christen, weitere Dozierende | |
Kurzbeschreibung | The lecture course provides an advanced understanding of modern techniques used in molecular microbiology. Current technologies and research directions in molecular microbiology including applied aspects will be illustrated with paper discussions. The format is a lecture course enriched by group activities. | |||||
Lernziel | The lecture course aims at providing principles of modern techniques used in molecular microbiology. Emphasis is on genetic, biochemical, and cellular analysis including also bioinformatics aspects. Discussion of a set of commonly applied technologies will assist students in evaluating current research in molecular microbiology and choosing appropriate methods for their own demands. | |||||
Inhalt | Important genetic, biochemical, biophysical, bioinformatic and structural analysis methods will be presented that are used to gain a deeper understanding of the molecular principles and mechanisms underlying basic physiological processes in prokaryotes. Applied aspects of molecular microbiology and current research in this area will also be covered. List of topics: - Analysis of genes, genomes and transcriptomes - Analysis of proteins, proteomes and microbial systems - Synthetic biology | |||||
Skript | Updated handouts will be provided during the class. | |||||
Literatur | Current literature references, relevant papers and handouts will be provided during the lectures. | |||||
Voraussetzungen / Besonderes | The following lecturers will contribute to the course: Prof. Beat Christen (ETH) Dr. Matthias Christen (ETH) Prof. Hans-Martin Fischer (ETH) Dr. Jonas Grossmann (FGCZ) Dr. Florian Freimoser (Agroscope) Dr. Bernd Roschitzki (FGCZ) Dr. Roman Spörri (ETH) | |||||
227-0390-00L | Elements of Microscopy | W | 4 KP | 3G | M. Stampanoni, G. Csúcs, A. Sologubenko | |
Kurzbeschreibung | The lecture reviews the basics of microscopy by discussing wave propagation, diffraction phenomena and aberrations. It gives the basics of light microscopy, introducing fluorescence, wide-field, confocal and multiphoton imaging. It further covers 3D electron microscopy and 3D X-ray tomographic micro and nanoimaging. | |||||
Lernziel | Solid introduction to the basics of microscopy, either with visible light, electrons or X-rays. | |||||
Inhalt | It would be impossible to imagine any scientific activities without the help of microscopy. Nowadays, scientists can count on very powerful instruments that allow investigating sample down to the atomic level. The lecture includes a general introduction to the principles of microscopy, from wave physics to image formation. It provides the physical and engineering basics to understand visible light, electron and X-ray microscopy. During selected exercises in the lab, several sophisticated instrument will be explained and their capabilities demonstrated. | |||||
Literatur | Available Online. | |||||
701-1708-00L | Infectious Disease Dynamics | W | 4 KP | 2V | S. Bonhoeffer, R. D. Kouyos, R. R. Regös, T. Stadler | |
Kurzbeschreibung | This course introduces into current research on the population biology of infectious diseases. The course discusses the most important mathematical tools and their application to relevant diseases of human, natural or managed populations. | |||||
Lernziel | Attendees will learn about: * the impact of important infectious pathogens and their evolution on human, natural and managed populations * the population biological impact of interventions such as treatment or vaccination * the impact of population structure on disease transmission Attendees will learn how: * the emergence spread of infectious diseases is described mathematically * the impact of interventions can be predicted and optimized with mathematical models * population biological models are parameterized from empirical data * genetic information can be used to infer the population biology of the infectious disease The course will focus on how the formal methods ("how") can be used to derive biological insights about the host-pathogen system ("about"). | |||||
Inhalt | After an introduction into the history of infectious diseases and epidemiology the course will discuss basic epidemiological models and the mathematical methods of their analysis. We will then discuss the population dynamical effects of intervention strategies such as vaccination and treatment. In the second part of the course we will introduce into more advanced topics such as the effect of spatial population structure, explicit contact structure, host heterogeneity, and stochasticity. In the final part of the course we will introduce basic concepts of phylogenetic analysis in the context of infectious diseases. | |||||
Skript | Slides and script of the lecture will be available online. | |||||
Literatur | The course is not based on any of the textbooks below, but they are excellent choices as accompanying material: * Keeling & Rohani, Modeling Infectious Diseases in Humans and Animals, Princeton Univ Press 2008 * Anderson & May, Infectious Diseases in Humans, Oxford Univ Press 1990 * Murray, Mathematical Biology, Springer 2002/3 * Nowak & May, Virus Dynamics, Oxford Univ Press 2000 * Holmes, The Evolution and Emergence of RNA Viruses, Oxford Univ Press 2009 | |||||
Voraussetzungen / Besonderes | Basic knowledge of population dynamics and population genetics as well as linear algebra and analysis will be an advantage. | |||||
751-4505-00L | Plant Pathology II | W | 2 KP | 2G | B. McDonald | |
Kurzbeschreibung | Plant Pathology II focuses on disease control in agroecosystems based on biological control, pesticide applications and breeding of resistant crop cultivars. The genetics of pathogen-plant interactions will be explored in detail as a basis for understanding the development of boom-and-bust cycles and methods that may be used to prevent the evolution of pathogen virulence and fungicide resistance. | |||||
Lernziel | An understanding of the how biological control, pesticides and plant breeding can be used to achieve sustainable disease control. An understanding of the genetic basis of pathogen-plant interactions and appropriate methods for using resistance to control diseases in agroecosystems. | |||||
Inhalt | Plant Pathology II will focus on disease control in agroecosystems based on biological control, pesticide applications and breeding of resistant crop cultivars. The genetics of pathogen-plant interactions will be explored in detail as a basis for understanding the development of boom-and-bust cycles and methods that may be used to prevent the evolution of pathogen virulence and fungicide resistance. Lecture Topics and Tentative Schedule Week 1 Biological control: biofumigation, disease declines, suppressive soils. Week 2 Biological control: competitive exclusion, hyperparasitism. Week 3 Chemical control: History of fungicides in Europe, fungicide properties, application methods. Week 4 Fungicide categories and modes of action, antibiotics, fungicide development, fungicide safety and risk assessment (human health). Week 5 Resistance to fungicides. Genetics of fungicide resistance, ABC transporters, risk assessment, fitness costs. FRAC risk assessment model vs. population genetic risk assessment model. Week 6 Genetics of pathogen-plant interaction: genetics of pathogens, genetics of plant resistance, major gene and quantitative resistance, acquired resistance. Flor's GFG hypothesis and the quadratic check, the receptor and elicitor model of GFG, the guard model of GFG. Week 7 Resistance gene structure and genome distribution, conservation of LRR motifs across eukaryotes. Genetic basis of quantitative resistance. QTLs and QRLs. Connections between MGR and QR. Durability of QR. Week 8 Genetic resistance: Costs, benefits and risks. Week 9 Non-host resistance. Types of NHR. NHR in Arabidopsis with powdery mildews. NHR in maize and rice. Avirulence genes and pathogen elicitors. PAMPs, effectors, type-III secretion systems, harpins in bacteria. Fungal avirulence genes. Week 10 Easter holiday no class. Week 11 Sechselauten holiday no class. Week 12 Host-specific toxins. GFG for toxins and connection to apoptosis. Fitness costs of virulence alleles. Diversifying selection in NIP1. Week 13 Boom and bust cycles for resistance genes and fungicides and coevolutionary processes. Pathogen genetic structure and evolutionary potential. Genetic structure of pathogen populations in agroecosystems, risk assessment for pathogen evolution and breeding strategies for durable resistance. Week 14 Resistance gene and fungicide deployment strategies for agroecosystems. Week 15 Genetic engineering approaches to achieve disease resistant crops. | |||||
Skript | Lecture notes will be available for purchase at the cost of reproduction. | |||||
Literatur | Lecture notes will be available for purchase at the cost of reproduction. | |||||
Voraussetzungen / Besonderes | Plant Pathology I provides a good preparation for Plant Pathology II, but is not a prerequisite for this course. | |||||
551-1700-00L | Introduction to Flow Cytometry Number of participants limited to 24. | W | 2 KP | 1V | J. Kisielow, L. Tortola, weitere Dozierende | |
Kurzbeschreibung | The lecture provides an introduction to flow cytometry. We will cover the technology basics, experimental design, data acquisition and analysis of flow and mass cytometry. In addition, various research applications will be discussed. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility and practical demonstration of the use of analysis and sorting instruments. | |||||
Lernziel | The goal of this course is to provide the basic knowledge of flow and mass cytometry required for planning and execution of cytometric experiments. | |||||
Inhalt | The lecture course aims at teaching principles of flow cytometry. The emphasis is on theoretical principles (signal detection, fluorochromes, signal spill-over and compensation) as well as practical aspects of experimental design and performance (sample preparation, controls, data acquisition and analysis). List of topics: - Principles of Flow Cytometry - Signal processing - Compensation and Controls - Data analysis, gating and presentation - Panel design - Sorting - Mass cytometry - High-dimensional data analysis - Practical demonstration (hardware and software) Modern flow cytometric techniques for immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be introduced. | |||||
Skript | Updated handouts will be provided during the class. | |||||
Literatur | Current literature references on immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be discussed during the lectures. | |||||
Zusätzliche Masterkurse | ||||||
Nummer | Titel | Typ | ECTS | Umfang | Dozierende | |
551-0512-00L | Current Topics in Molecular and Cellular Neurobiology Findet dieses Semester nicht statt. Number of participants limited to 8 | W | 2 KP | 1S | U. Suter | |
Kurzbeschreibung | The course is a literature seminar or "journal club". Each Friday a student, or a member of the Suter Lab in the Institute of Molecular Health Sciences, will present a paper from the recent literature. | |||||
Lernziel | The course introduces you to recent developments in the fields of cellular and molecular neurobiology. It also supports you to develop your skills in critically reading the scientific literature. You should be able to grasp what the authors wanted to learn e.g. their goals, why the authors chose the experimental approach they used, the strengths and weaknesses of the experiments and the data presented, and how the work fits into the wider literature in the field. You will present one paper yourself, which provides you with practice in public speaking. | |||||
Inhalt | You will present one paper yourself. Give an introduction to the field of the paper, then show and comment on the main results (all the papers we present are available online, so you can show original figures with a beamer). Finish with a summary of the main points and a discussion of their significance. You are expected to take part in the discussion and to ask questions. To prepare for this you should read all the papers beforehand (they will be announced a week in advance of the presentation). | |||||
Skript | Presentations will be made available after the seminars. | |||||
Literatur | We cover a range of themes related to development and neurobiology. Before starting your preparations, you are required to check with Laura Montani (Link), who helps you with finding an appropriate paper. | |||||
Voraussetzungen / Besonderes | You must attend at least 80% of the journal clubs, and give a presentation of your own. At the end of the semester there will be a 30 minute oral exam on the material presented during the semester. The grade will be based on the exam (45%), your presentation (45%), and a contribution based on your active participation in discussion of other presentations (10%). | |||||
551-0224-00L | Advanced Proteomics Für Masterstudierende ab 2. Semester, Doktorierende und Postdoktorierende | W | 4 KP | 6G | R. Aebersold, L. Gillet, M. Gstaiger, A. Leitner, P. Pedrioli | |
Kurzbeschreibung | Ziel dieses Kurses ist es, etablierte und neue Technologien der Protein- und Proteome-Analyse kennenzulernen in Bezug auf ihre Anwendung in Biologie, Biotechnologie und Medizin. Format: Einführung durch Dozent mit anschliessender Diskussion, unterstützt durch Literaturarbeit und Übungen. | |||||
Lernziel | Im Kurs werden sowohl die bereits etablierten als auch die neuesten derzeit entstehenden Technologien und Methoden in der Protein- und Proteomanlayse diskutiert im Hinblick auf ihre Anwendung in der Biologie, Biotechnologie, Medizin und Systembiologie. | |||||
Inhalt | Block course teaching current methods for the acquisition and processing of proteomic datasets. | |||||
Voraussetzungen / Besonderes | Number of people: Not exceeding 30. Students from ETHZ, Uni Zurich and University of Basel Non-ETH students must register at ETH Zurich as special students Link | |||||
Zusätzliche Konzeptkurse | ||||||
Nummer | Titel | Typ | ECTS | Umfang | Dozierende | |
752-4006-00L | Lebensmittel-Mikrobiologie II | W | 3 KP | 2V | M. Loessner, J. Klumpp | |
Kurzbeschreibung | Vermittlung von (teilweise vertieften) Basiskenntnissen ueber Methoden fuer Nachweis und die Differenzierung von (nicht nur lebensmittelrelevanten) Mikroorganismen; Herstellung von Lebensmitteln mit Mikroorganismen; Haltbarmachung und Lebensmittelsicherheit; kurzer Ueberblick ueber gesetzliche Regelungen und Hygienemassnahmen. | |||||
Lernziel | Der zweite Teil dieser 1 Jahres-Vorlesung vermittelt (teilweise vertiefte) Basiskenntnisse ueber verschiedene Methoden (klassisch und molekularbiologisch)fuer den Nachweis und die Differenzierung von (nicht nur lebensmittelrelevanten) Mikroorganismen; die Herstellung von Lebensmitteln mit Mikroorganismen; verschiedene Ansaetze zur Haltbarmachung und Lebensmittelsicherheit; und einen kurzen Ueberblick ueber gesetzliche Regelungen und Hygienemassnahmen. | |||||
Inhalt | Nachweis und Differenzierung von Mikroorganismen Kulturmethoden, Mikroskopischer Nachweis, Anreicherung und Separation, Nachweis intrazellulärer Metaboliten und Enzyme, Immunologische Methoden, Gensonden und Microarrays, Nukleinsäureamplifikation, Expression von Reportergenen, Typisierungsmethoden Herstellung von Lebensmitteln mit Mikroorganismen Fermentierte pflanzliche Produkte, Brot und Sauerteig, Fermentierte (alkoholische) Getränke, Fermentierte Milchprodukte, Probiotika, Fermentierte Fleischprodukte, Traditionelle Fermentationsprodukte, Kaffee, Tee, Kakao, Tabak; Störungen der Fermentation (Viren, Antibiotika, Desinfektionsmittel) Haltbarmachung I: Physikalische Verfahren Erniedrigung der Wasseraktivität, Erniedrigung der Temperatur, Hitzebehandlung, Hochdruckbehandlung, Bestrahlung Haltbarmachung II. Chemische Verfahren Natürliche antimikrobielle Stoffe, Räuchern, Konservierungsstoffe, Erniedrigung des pH Wertes, Schutzgas- und Vakuumverpackung Haltbarmachung III. Biologische Verfahren Zusatz von Enzymen, Schutzkulturen, Starter- und Reifungskulturen Qualitätssicherung und Kontrolle Gesetzliche Kriterien & Verordnungen, Betriebs- & Personalhygiene, Reinigung & Desinfektion, GHP & HACCP | |||||
Skript | Elektronische PDF Kopien der Praesentationsfolien werden an die Studenten abgegeben | |||||
Literatur | Hinweise in der ersten Vorlesungsstunde | |||||
Voraussetzungen / Besonderes | Die Vorlesung "Lebensmittelmikrobiologie I" (oder eine ähnliche Veranstaltung) wird inhaltlich vorausgesetzt | |||||
529-0732-00L | Proteins and Lipids | W | 6 KP | 3G | D. Hilvert | |
Kurzbeschreibung | An overview of the relationship between protein sequence, conformation and function. | |||||
Lernziel | Overview of the relationship between protein sequence, conformation and function. | |||||
Inhalt | Proteins, structures and properties, (bio)synthesis of polypeptides, protein folding and design, protein engineering, chemical modification of proteins, proteomics. | |||||
Literatur | General Literature: - T.E. Creighton: Proteins: Structures and Molecular Properties, 2nd Edition, H.W. Freeman and Company, New York, 1993. - C. Branden, J. Tooze , Introduction to Protein Structure, Garland Publishing, New York, 1991. - J. M. Berg, J. L. Tymoczko, L. Stryer: Biochemistry, 5th edition, H.W. Freeman and Company, New York, 2002. - G.A. Petsko, D. Ringe: Protein Structure and Function, New Science Press Ltd., London, 2004. Original Literature: Citations from the original literature relevant to the individual lectures will be assigned weekly. | |||||
551-0326-00L | Cell Biology | W | 6 KP | 4V | S. Werner, M. Bordoli, R. Henneberger, W. Kovacs, M. Schäfer, U. Suter, A. Wutz | |
Kurzbeschreibung | This Course introduces principle concepts, techniques, and experimental strategies used in modern Cell Biology. Major topics include: neuron-glia interactions in health and disease; mitochondrial dynamics; stem cell biology; growth factor action in development, tissue repair and disease; cell metabolism, in particular sensing and signaling mechanisms, cell organelles, and lipid metabolism. | |||||
Lernziel | -To prepare the students for successful and efficient lab work by learning how to ask the right questions and to use the appropriate techniques in a research project. -To convey knowledge about neuron-glia interactions in health and disease. - To provide information on different types of stem cells and their function in health and disease -To provide information on growth factor signaling in development, repair and disease and on the use of growth factors or their receptors as drug targets for major human diseases -To convey knowledge on the mechanisms underlying repair of injured tissues -To provide the students with an overview of mitochondrial dynamics. -Providing an understanding of RNA processing reactions and their regulations. -To provide a comprehensive understanding of metabolic sensing mechanisms occurring in different cell types and organelles in response to glucose, hormones, oxygen, nutrients as well as lipids, and to discuss downstream signaling pathways and cellular responses. -To provide models explaining how disturbances in complex metabolic control networks and bioenergetics can lead to disease and to highlight latest experimental approaches to uncover the intricacies of metabolic control at the cellular and organismal level. -Providing the background and context that foster cross-disciplinary scientific thinking. | |||||
551-0324-00L | Systems Biology | W | 6 KP | 4V | R. Aebersold, B. Christen, M. Claassen, U. Sauer | |
Kurzbeschreibung | Introduction to experimental and computational methods of systems biology. By using baker’s yeast as a thread through the series, we focus on global methods for analysis of and interference with biological functions. Illustrative applications to other organisms will highlight medical and biotechnological aspects. | |||||
Lernziel | - obtain an overview of global analytical methods - obtain an overview of computational methods in systems biology - understand the concepts of systems biology | |||||
Inhalt | Overview of global analytical methods (e.g. DNA arrays, proteomics, metabolomics, fluxes etc), global interference methods (siRNA, mutant libraries, synthetic lethality etc.) and imaging methods. Introduction to mass spectrometry and proteomics. Concepts of metabolism in microbes and higher cells. Systems biology of developmental processes. Concepts of mathematical modeling and applications of computational systems biology. | |||||
Skript | no script | |||||
Literatur | The course is not taught by a particular book, but some books are suggested for further reading: - Systems biology in Practice by Klipp, Herwig, Kowald, Wierling und Lehrach. Wiley-VCH 2005 | |||||
551-0320-00L | Cellular Biochemistry (Part II) | W | 3 KP | 2V | Y. Barral, R. Kroschewski, A. E. Smith | |
Kurzbeschreibung | This course will focus on molecular mechanisms and concepts underlying cellular biochemistry, providing advanced insights into the structural and functional details of individual cell components, and the complex regulation of their interactions. Particular emphasis will be on the spatial and temporal integration of different molecules and signaling pathways into global cellular processes. | |||||
Lernziel | The full-year course (551-0319-00 & 551-0320-00) focuses on the molecular mechanisms and concepts underlying the biochemistry of cellular physiology, investigating how these processes are integrated to carry out highly coordinated cellular functions. The molecular characterization of complex cellular functions requires a combination of approaches such as biochemistry, but also cell biology and genetics. This course is therefore the occasion to discuss these techniques and their integration in modern cellular biochemistry. The students will be able to describe the structural and functional details of individual cell components, and the spatial and temporal regulation of their interactions. In particular, they will learn to explain how different molecules and signaling pathways can be integrated during complex and highly dynamic cellular processes such as intracellular transport, cytoskeletal rearrangements, cell motility, and cell division. In addition, they will be able to illustrate the relevance of particular signaling pathways for cellular pathologies such as cancer or during cellular infection. | |||||
Inhalt | Spatial and temporal integration of different molecules and signaling pathways into global cellular processes, such as cell division, cell infection and cell motility. Emphasis is also put on the understanding of pathologies associated with defective cell physiology, such as cancer or during cellular infection. | |||||
Literatur | Recommended supplementary literature (review articles and selected primary literature) will be provided during the course. | |||||
Voraussetzungen / Besonderes | To attend this course the students must have a solid basic knowledge in chemistry, biochemistry, cell biology and general biology. Biology students have in general already attended the first part of the "Cellular Biochemistry" concept course (551-0319-00). The course will be taught in English. In addition, the course will be based on a blended-learning scenario, where frontal lectures will be complemented with carefully chosen web-based teaching elements that students access through the ETH Moodle platform. | |||||
551-0307-01L | Molecular and Structural Biology II: From Gene to Protein D-BIOL students are obliged to take part I and part II as a two-semester course. | W | 3 KP | 2V | N. Ban, F. Allain, S. Jonas, M. Pilhofer | |
Kurzbeschreibung | This course will cover advanced topics in molecular biology and biochemistry with emphasis on the structure and function of cellular assemblies involved in expression and maintenance of genetic information. We will cover the architecture and the function of molecules involved in DNA replication, transcription, translation, nucleic acid packaging in viruses, RNA processing, and CRISPER/CAS system. | |||||
Lernziel | Students will gain a deep understanding of large cellular assemblies and the structure-function relationships governing their function in fundamental cellular processes ranging from DNA replication, transcription and translation. The lectures throughout the course will be complemented by exercises and discussions of original research examples to provide students with a deeper understanding of the subjects and to encourage active student participation. | |||||
Inhalt | Advanced class covering the state of the research in structural molecular biology of basic cellular processes with emphasis on the function of large cellular assemblies. | |||||
Skript | Updated handouts will be provided during the class. | |||||
Literatur | The lecture will be based on the latest literature. Additional suggested literature: Branden, C., and J. Tooze, Introduction to Protein Structure, 2nd ed. (1995). Garland, New York. |