Search result: Catalogue data in Spring Semester 2018

Biotechnology Master Information
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Master Studies (Programme Regulations 2017)
Practical Training
Students need to acquire a total of 14 ECTS in lab courses.
All listed lab courses are mandatory.
NumberTitleTypeECTSHoursLecturers
636-0207-00LLab Course: Cellular Engineering Stem Cells Restricted registration - show details
Only for Biotechnology MSc, Programme Regulations 2017.
Attention: This lab course was offered in previous semesters with the number: 626-0806-00L "Laboratory Course Stem Cell Purification, Culture and Manipulation”. Students that already passed course 626-0806-00L cannot receive credits for course 636-0207-00L.
O2 credits6PT. Schroeder
AbstractMammalian stem cells of different organs are purified, cultured, differentiated, analyzed and manipulated. Plasmids and viral vectors will be cloned, produced and transfected / transduced to manipulate stem cells. Computational and analytical molecular biology methods, FACS and imaging and lectures complement the program.
ObjectiveIndependent planning and conducting of experiments with mammalian stem cells including all steps from culturing different cell lines to DNA transfection / transduction and expression analysis by different analytical methods. Documenting and writing a report on conducted experiments and results.
ContentPractical course on purification of primary mammalian stem cells, culture of primary stem cells and stem cell lines, characterization, manipulation and differentiation of stem cells. Construction of plasmids or viral vectors for gene expression, DNA transfer by transfection and transduction, analysis of gene expression by fluorescent proteins, PCR, fluorescence-activated cell sorting (FACS), imaging. Documentation of experiments in a laboratory journal, writing of a report on the experiments and results.
636-0206-00LLab Course: Cellular Engineering Mammalian Cells Restricted registration - show details
Only for Biotechnology MSc, Programme Regulations 2017
Attention: This lab course was offered in previous semesters with the number: 626-0802-00L "Practical Course in Mammalian Cell Biotechnology”. Students that already passed course 626-0802-00L cannot receive credits for course 636-0206-00L.
O2 credits6PM. Fussenegger, M. Folcher
AbstractMammalian cells will be transfected and transduced for the production of biopharmaceuticals, for drug discovery as well as for the design of synthetic biology-inspired programmable gene circuits. A wide array of analytical techniques, lectures, and excursions to biotech companies will complement the practical part.
ObjectiveIndependent planning and conducting of experiments with mammalian cells including all steps from culturing different cell lines to DNA transfection/transduction and expression analysis using a wide array of analytical methods.
ContentA practical course on characterization and cultivation of mammalian cells, DNA transfer by transfection, construction of synthetic gene networks, analysis of gene expression by enzymatic and immunological methods and fluorescent proteins, bioprocessing, mammalian cell-based assays for drug discovery and diagnostics. Excursions to Biotech/Pharma companies.
Lecture notesWill be distributed on first day of the practical course
636-0204-00LLab Course: Microbial Biotechnology Restricted registration - show details
Only for Biotechnology MSc, Programme Regulations 2017.
O2 credits5PM. Held
AbstractStudents will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
ObjectiveStudents will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
ContentBlock A: Handling and preparation and of microbial libraries
D1: Introduction to microbiological cultures and monoseptic working techniques.
D2: Plasmid-based expression systems and variation of XFP synthesis levels via site-directed RBS mutagenesis.
Block B: Library screening
D3: In vivo screening for XFP expression levels.
D4: Analysis of XFP levels via SDS-PAGE analysis. RBS-sequencing.
Block C: Hit recovery and validation
D5: In silico analysis of RBS variants.
D6: Cellular XFP content for selected variants at different culture conditions.
Block D: Data analysis and presentation
D7: Protein expression analysis. Q&A for reports and presentations.
D8: Final presentations and wrap-up.
Lecture notesMaterial will be provided during the course.
Literature(1) Reetz MT, Kahakeaw D, and Lohmer R. "Addressing the numbers problem in directed evolution." ChemBioChem 2008
(2) Jeschek M, Gerngross D, and Panke S. “Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.” Nat. Commun. 2016
(3) Salis HM. “The ribosome binding site calculator.” Methods Enzymol. 2011
(4) Nienhaus G, Nienhaus K, and Wiedenmann J. "Structure–Function Relationships in Fluorescent Marker Proteins of the Green Fluorescent Protein Family." Fluorescent Proteins I. Springer Berlin Heidelberg, 2011

General introduction to microbiology:

(5) Schlegel HG, and Zaborosch C. “General Microbiology.” Cambridge University Press 1993
(6) Pirt JS. “Principles of microbe and cell cultivation.” Blackwell Scientific Publications 1975
636-0205-00LLab Course: Mammalian Gene Circuits Restricted registration - show details
Only for Biotechnology MSc, Programme Regulations 2017.
O2 credits5PY. Benenson
AbstractThe students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level.
ObjectiveThe objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization.
ContentThe course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities

Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1.
Week 2: Cloning of the constructs, purification and characterization of DNA constructs
Week 3: Cell culture transfection, microscopy and flow cytometry characterization
Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments.
Lecture notesPreparatory materials will be provided before the start of the course.
LiteratureWill be provided before the course
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