Suchergebnis: Katalogdaten im Frühjahrssemester 2023
Biologie Master  | ||||||||||||||||||||||||||||||||||||||||||
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| Wahlvertiefung: Mikrobiologie und Immunologie | ||||||||||||||||||||||||||||||||||||||||||
| Wahlpflicht Masterkurse | ||||||||||||||||||||||||||||||||||||||||||
| Nummer | Titel | Typ | ECTS | Umfang | Dozierende | |||||||||||||||||||||||||||||||||||||
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| 701-1310-00L | Environmental Microbiology | W | 3 KP | 2V | M. H. Schroth, H. Bürgmann | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Microorganisms catalyze a large number of reactions that are of great importance to terrestrial and aquatic environments. To improve our understanding of the dynamics of a specific environment, it is important to gain a better understanding of microbial structures and their functions under varying environmental conditions. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Students will learn basic concepts in microbial ecology. Qualitative and quantitative concepts will be presented to assess microbial communities and associated processes in terrestrial and aquatic environments. Microbial diversity in such ecosystems will be illustrated in discussions of selected habitats. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Lectures will cover general concepts of environmental microbiology including (i) quantification of microbial processes, (ii) energy fluxes in microbial ecosystems, (iii) application of state-of-the-art microbiological and molecular tools, and (iv) use of isotope methods for identification of microbial structures and functions. Topics to illustrate the microbial diversity of terrestrial and aquatic ecosystems will include (i) interactions between microbes and mineral/metallic solid phases, (ii) microbial carbon and nutrient cycling, (iii) microbial processes involved in the turnover of greenhouse gases, (iv) biofilms and microbial mats, (v) bioremediation, (vi) microorganisms in extreme habitats, and (vii) microbial evolution and astrobiology. | |||||||||||||||||||||||||||||||||||||||||
| Skript | available at time of lecture - will be distributed electronically as pdf's | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Brock Biology of Microorganisms, Madigan M. et al., Pearson, 14th ed., 2015 | |||||||||||||||||||||||||||||||||||||||||
| 551-1100-00L | Infectious Agents: From Molecular Biology to Disease Number of participants limited to 22. Requires application until 2 weeks before the start of the semester; selected applicants will be notified one week before the first week of lectures. (if you missed the deadline, please come to the first date to see, if there are any slots left) | W | 4 KP | 2S | W.‑D. Hardt, A. B. Hehl, M. Kopf, S. R. Leibundgut, C. Münz, A. Oxenius, P. Sander, weitere Dozierende | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Literature seminar for students at the masters level and PhD students. Introduction to the current research topics in infectious diseases; Introduction to key pathogens which are studied as model organisms in this field; Overview over key research groups in the field of infectious diseases in Zürich. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Working with the current research literature. Getting to know the key pathogens serving as model organisms and the research technologies currently used in infection biology. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | for each model pathogen (or key technology): 1. introduction to the pathogen 2. Discussion of one current research paper. The paper will be provided by the respective supervisor. He/she will give advice (if required) and guide the respective literature discussion. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Teachers will provide the research papers to be discussed. Students will prepare handouts for the rest of the group for their assigned seminar. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Teachers will provide the research papers to be discussed. | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Restricted to max 22 students. Please sign up until two weeks before the beginning of the semester via e-mail to micro_secr@micro.biol.ethz.ch and include the following information: 551-1100-00L; your name, your e-mail address, university/eth, students (specialization, semester), PhD students (research group, member of a PhD program? which program?). The 22 students admitted to this seminar will be selected and informed by e-mail in the week befor the beginning of the semester by W.-D. Hardt. The first seminar date will serve to form groups of students and assign a paper to each group. | |||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1118-00L | Cutting Edge Topics: Immunology and Infection Biology II | W | 2 KP | 2S | A. Oxenius, B. Becher, C. Halin Winter, N. C. Joller, M. Kopf, S. R. Leibundgut, C. Münz, F. Sallusto, C. Schneider, R. Spörri, M. van den Broek, Uni-Dozierende | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. Ziel der Veranstaltung ist die Konfrontation von Studenten und Doktoranden mit aktuellen Forschungsthemen und mit wissenschaftlicher Vortragsform. Studenten und Doktoranden wird die Gelegenheit geboten, sich mit diversen Themen vertieft auseinander zu setzen, welche oft in den Konzeptkursen nur knapp präsentiert werden und mit Experten auf dem Gebiet zu diskutieren. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Immunologie und Infektionsbiologie. Die speziellen Themen variieren jedes Semester und hängen von den eingeladenen Experten ab. | |||||||||||||||||||||||||||||||||||||||||
| 551-1104-00L | Ausgewählte Kapitel der Mykologie im Wald Findet dieses Semester nicht statt. | W | 2 KP | 1V | M. Peter Baltensweiler, S. Prospero | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Lebensweisen und Funktionen von symbiotischen, saproben und pathogenen Pilzen, Lebensgemeinschaften der Mykorrhiza und funktionelle Aspekte der Mykorrhizadiversität, Evolution und phylogenetische Aspekte der Pflanzen-Pilz Interaktionen, inter- und intraspezifische Myzelinteraktionen, Rolle der Pilze bei Nährstofferschliessung und Verwitterung. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Vertiefte Kenntnisse der Biologie und Ökologie der Pilze im Wald. Selbständige Auseinandersetzung mit aktueller Literatur. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Vertiefte Behandlung ausgewählter Themen der Pilze im Ökosystem Wald: Lebensweisen und Funktionen von symbiotischen, saproben und pathogenen Pilzen, Lebensgemeinschaften der Mykorrhiza und funktionelle Aspekte der Mykorrhizadiversität, Evolution und phylogenetische Aspekte der Pflanzen-Pilz Interaktionen, Inter- und intraspezifische Myzelinteraktionen, Rolle der Pilze bei Nährstofferschliessung und Verwitterung. Die Grundlagen werden in Vorlesungen vermittelt. Daneben selbständige Vertiefung des Stoffes mit Hilfe aktueller Literatur und Präsentationen. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Unterlagen zum Kurs werden abgegeben. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Smith S.E. and Read D.J. 1997. Mycorrhizal Symbiosis. Academic Press, 2nd ed., pp. 605. | |||||||||||||||||||||||||||||||||||||||||
| 551-0216-00L | Mykologischer Feldkurs  | W | 3 KP | 5P | R. Berndt, M. A. Garcia Otalora | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Die Studierenden lernen die Arten- und Formenvielfalt der Asco- und Basidiomyceten bei mehreren Tagesexkursionen und der Bearbeitung der Funde im Labor kennen. Sie lernen, wie man Pilze für wissenschaftliche Zwecke sammelt, mikroskopiert und bestimmt. Die Teilnehmer/innen erhalten zudem eine theoretische Einführung in die behandelten Pilzgruppen und die Praxis der Pilzmikroskopie. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Erwerb von Artenkenntnis bei Pilzen. Sammeln, Dokumentieren und Herbarisieren von Pilzen für wissenschaftliche Zwecke. Methoden der Pilzmikroskopie. Umgang mit mykologischer Bestimmungsliteratur. Erlernen der bestimmungswichtigen makroskopischen und mikroskopischen Merkmale der Pilze und der notwendigen Fachterminologie. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Einführung in die Systematik der Asco- und Basidiomyceten. Exkursionen zum Kennenlernen von Pilzen am Standort. Untersuchung und Bestimmung der Funde im Kursraum. Makroskopische und mikroskopische Merkmale von Lichenisierten Pilzen (Flechten), Basidiomyceten, Grossgruppen der pflanzenparasitischen Pilze (v. a. Rostpilze). | |||||||||||||||||||||||||||||||||||||||||
| Skript | Kursunterlagen werden abgegeben | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Spezialliteratur für die Bestimmung wird bereitgestellt. | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Der Kurs ist auf maximal acht Teilnehmende beschränkt. Schriftliche definitive Anmeldung bei den Dozierenden erforderlich. Voraussetzung: Die Teilnehmerinnen und Teilnehmer lesen vor dem Kurs ausgewählte Lehrbuchkapitel (nach Vereinbarung) und erarbeiten sich die für den Kurs erforderlichen mykologischen Basiskenntnisse. Fahrtkosten von ca. 100-120 CHF (Halbtax) fallen für die Exkursionen an. | |||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1132-00L | Allgemeine Virologie | W | 2 KP | 1V | K. Tobler, C. Fraefel | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Einführung in die Grundlagen der Virologie, welche die Charakterisierung von Viren, die Interaktionen der Viren mit infizierten Zellen, Wirten und Populationen, die Grundlagen des Schutzes vor Infektion und die Virusdiagnostik beinhaltet. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Einführung in die Grundlagen der Virologie. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Grundlagen der Virologie. Charakterisierung von Viren. Virus-Zell-Interaktionen. Virus-Wirt-Interaktionen. Virus-Population-Interaktionen. Schutz vor Virusinfektion. Virusdiagnostik. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Die Vorlesung ist auf dem Lehrbuch "Allgemeine Virologie" von Kurt Tobler, Mathias Ackermann und Cornel Fraefel aufgebaut. Die Präsentationsfolien und ausgewählte Primärliteratur werden 24 bis 48 Stunden vor den Lektionen als .pdf-Dateien bereitgestellt. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Kurt Tobler, Mathias Ackermann und Cornel Fraefel, Allgemeine Virologie, 2021, 2. Auflage UTB-Band-Nr.:4516 Haupt Verlag Bern ISBN: 978-3-8252-5630-2 | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Grundkenntnisse in Molekularbiologie, Zellbiologie und Immunologie | |||||||||||||||||||||||||||||||||||||||||
| 551-0140-00L | Epigenetics | W | 4 KP | 2V | A. Wutz, U. Grossniklaus, R. Santoro, F. von Meyenn | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Epigenetik untersucht die Vererbung von Merkmalen, die nicht auf eine Veränderung der DNA Sequenz zurückgeführt werden kann. Die Vorlesung gibt einen Überblick über epigenetische Phänomene und erklärt die zugrundeliegenden molekularen Mechanismen. Die Rolle von epigenetischen Prozessen bei der Krebsentstehung und anderen Krankheiten wird diskutiert. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Das Ziel des Kurses ist das Verständnis von epigenetischen Mechanismen und deren Funktion in der Entwicklung von Organismen, bei Regenerationsprozessen oder bei der Entstehung von Krankheiten. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Themen - Historischer Überblick, Konzepte und Vergleich Genetik vs. Epigenetik - Biologie von Chromatin: Struktur und Funktion, Organisation im Kern und die Rolle von Histon Modifikationen bei Prozessen wie Transkription und Replikation. - DNA-Methylierung als epigenetische Modifikation - Weitergabe epigenetischer Modifikationen während der Zellteilung: das Zellgedächtnis - Stabilität/Revertierbarkeit epigenetischer Modifikationen: zelluläre Plastizität und Stammzellen. - Genomisches Imprinting in Pflanzen und in Säugern - X Chromosom Inaktivierung und Dosiskompensation - Positionseffekte, Paramutationen und Transvektion - RNA-induziertes Gensilencing - die Rolle von epigenetischen Prozessen bei der Krebsentstehung oder der Zellalterung. | |||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 751-4904-00L | Microbial Pest Control | W | 2 KP | 2G | J. Enkerli, G. Grabenweger, J. Mayerhofer | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Die Vorlesung vermittelt konzeptionelle, sowie biologische und ökologische Grundlagen in mikrobieller Schädlingsbekämpfung. Anhand von Beispielen werden die Methoden und Techniken zur Entwicklung und Überwachung von mikrobiellen Schädlingsbekämpfungsmitteln erarbeitet. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Kennenlernen der wichtigsten Gruppen von insektenpathogenen Mikroorganismen und deren Eigenschaften. Vertraut werden mit den nötigen Schritten für die Entwicklung von Schädlingsbekämpfungsmitteln. Verstehen der Techniken und Methoden, die für das Überwachen von Feldapplikationen benützt werden, und Kennen der Registrierungsanforderungen für mikrobielle Schädlingsbekämpfungsmittel. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Die in der biologischen Schädlingsbekämpfung gebrauchten Definitionen und generell verwendete Ausdrücke werden erarbeitet. Ferner werden biologische und ökologische Aspekte aller Arthropoden-pathogenen Gruppen (Viren, Bakterien Pilze und Nematoden) und ihre Vor- und Nachteile in Bezug auf biologische Schädlingsbekämpfung diskutiert. Ein Schwergewicht wird dabei auf die Pilzgruppen Hypocreales und Entomophtorales gelegt. Anhand von Beispielen wird aufgezeigt, wie Projekte in biologischer Schädlingsbekämpfung aufgebaut werden können, wie Pathogene appliziert werden und wie die Effizienz, Effekte auf Nicht-Zielorganismen, Persistenz und Verbreitung überwacht werden. Im Weiteren werden die nötigen Schritte in der Entwicklung eines Produktes, kommerzielle Aspekte und die Registrierungsanforderungen besprochen. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Die grundlegenden Aspekte werden als Skript (Präsentationsunterlagen) abgegeben. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Hinweise auf zusätzliche Literatur werde in der Lehrveranstaltung gegeben. | |||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1126-00L | Technologies in Molecular Microbiology | W | 4 KP | 2V | B. Nguyen, W.‑D. Hardt, weitere Dozierende | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | The lecture course provides an advanced understanding of modern techniques used in molecular microbiology. Current technologies and research directions in molecular microbiology including applied aspects will be illustrated with paper discussions. The format is a lecture course enriched by group activities. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | The lecture course aims at providing principles of modern techniques used in molecular microbiology. Emphasis is on genetic, biochemical, cellular, and community analysis . Discussion of a set of commonly applied technologies will assist students in evaluating current research in molecular microbiology and choosing appropriate methods for their own demands. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Important genetic, biochemical, biophysical, and community analysis methods will be presented that are used to gain a deeper understanding of the molecular principles and mechanisms underlying basic physiological processes in prokaryotes. Applied aspects of molecular microbiology and current research in this area will also be covered. List of topics: - Analysis of genes, genomes and transcriptomes - Analysis of proteins, proteomes and microbial systems | |||||||||||||||||||||||||||||||||||||||||
| Skript | Updated handouts will be provided during the class. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Current literature references, relevant papers and handouts will be provided during the lectures. | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | The following lecturers will contribute to the course: Dr. Alex Brachmann (ETH) Prof. Hans-Martin Fischer (ETH) Dr. Florian Freimoser (Agroscope) Dr. Jonas Grossmann (FGCZ) Annika Hausmann (ETH) Dr. Bidong Nguyen (ETH) Dr. Bernd Roschitzki (FGCZ) Dr. Roman Spörri (ETH) | |||||||||||||||||||||||||||||||||||||||||
| 227-0390-00L | Elements of Microscopy | W | 4 KP | 3G | M. Stampanoni, G. Csúcs, A. Sologubenko | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | The lecture reviews the basics of microscopy by discussing wave propagation, diffraction phenomena and aberrations. It gives the basics of light microscopy, introducing fluorescence, wide-field, confocal and multiphoton imaging. It further covers 3D electron microscopy and 3D X-ray tomographic micro and nanoimaging. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Solid introduction to the basics of microscopy, either with visible light, electrons or X-rays. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | It would be impossible to imagine any scientific activities without the help of microscopy. Nowadays, scientists can count on very powerful instruments that allow investigating sample down to the atomic level. The lecture includes a general introduction to the principles of microscopy, from wave physics to image formation. It provides the physical and engineering basics to understand visible light, electron and X-ray microscopy. During selected exercises in the lab, several sophisticated instrument will be explained and their capabilities demonstrated. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Available Online. | |||||||||||||||||||||||||||||||||||||||||
| 701-1708-00L | Infectious Disease Dynamics | W | 4 KP | 2V | R. R. Regös, S. Bonhoeffer, R. D. Kouyos, T. Stadler | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This course introduces into current research on the population biology of infectious diseases. The course discusses the most important mathematical tools and their application to relevant diseases of human, natural or managed populations. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Attendees will learn about: * the impact of important infectious pathogens and their evolution on human, natural and managed populations * the population biological impact of interventions such as treatment or vaccination * the impact of population structure on disease transmission Attendees will learn how: * the emergence spread of infectious diseases is described mathematically * the impact of interventions can be predicted and optimized with mathematical models * population biological models are parameterized from empirical data * genetic information can be used to infer the population biology of the infectious disease The course will focus on how the formal methods ("how") can be used to derive biological insights about the host-pathogen system ("about"). | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | After an introduction into the history of infectious diseases and epidemiology the course will discuss basic epidemiological models and the mathematical methods of their analysis. We will then discuss the population dynamical effects of intervention strategies such as vaccination and treatment. In the second part of the course we will introduce into more advanced topics such as the effect of spatial population structure, explicit contact structure, host heterogeneity, and stochasticity. In the final part of the course we will introduce basic concepts of phylogenetic analysis in the context of infectious diseases. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Slides and script of the lecture will be available online. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | The course is not based on any of the textbooks below, but they are excellent choices as accompanying material: * Keeling & Rohani, Modeling Infectious Diseases in Humans and Animals, Princeton Univ Press 2008 * Anderson & May, Infectious Diseases in Humans, Oxford Univ Press 1990 * Murray, Mathematical Biology, Springer 2002/3 * Nowak & May, Virus Dynamics, Oxford Univ Press 2000 * Holmes, The Evolution and Emergence of RNA Viruses, Oxford Univ Press 2009 | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Basic knowledge of population dynamics and population genetics as well as linear algebra and analysis will be an advantage. | |||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 751-4505-00L | Plant Pathology II | W | 2 KP | 2G | B. McDonald | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Plant Pathology II focuses on disease control in agroecosystems based on biological control, pesticide applications and breeding of resistant crop cultivars. The genetics of pathogen-plant interactions will be explored in detail as a basis for understanding the development of boom-and-bust cycles and methods that may be used to prevent the evolution of pathogen virulence and fungicide resistance. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | An understanding of the how biological control, pesticides and plant breeding can be used to achieve sustainable disease control. An understanding of the genetic basis of pathogen-plant interactions and appropriate methods for using resistance to control diseases in agroecosystems. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Plant Pathology II will focus on disease control in agroecosystems based on biological control, pesticide applications and breeding of resistant crop cultivars. The genetics of pathogen-plant interactions will be explored in detail as a basis for understanding the development of boom-and-bust cycles and methods that may be used to prevent the evolution of pathogen virulence and fungicide resistance. Lecture Topics and Tentative Schedule Week 1 Biological control: biofumigation, disease declines, suppressive soils. Week 2 Biological control: competitive exclusion, hyperparasitism. Week 3 Chemical control: History of fungicides in Europe, fungicide properties, application methods. Week 4 Fungicide categories and modes of action, antibiotics, fungicide development, fungicide safety and risk assessment (human health). Week 5 Resistance to fungicides. Genetics of fungicide resistance, ABC transporters, risk assessment, fitness costs. FRAC risk assessment model vs. population genetic risk assessment model. Week 6 Genetics of pathogen-plant interaction: genetics of pathogens, genetics of plant resistance, major gene and quantitative resistance, acquired resistance. Flor's GFG hypothesis and the quadratic check, the receptor and elicitor model of GFG, the guard model of GFG. Week 7 Resistance gene structure and genome distribution, conservation of LRR motifs across eukaryotes. Genetic basis of quantitative resistance. QTLs and QRLs. Connections between MGR and QR. Durability of QR. Week 8 Genetic resistance: Costs, benefits and risks. Week 9 Non-host resistance. Types of NHR. NHR in Arabidopsis with powdery mildews. NHR in maize and rice. Avirulence genes and pathogen elicitors. PAMPs, effectors, type-III secretion systems, harpins in bacteria. Fungal avirulence genes. Week 10 Easter holiday no class. Week 11 Sechselauten holiday no class. Week 12 Host-specific toxins. GFG for toxins and connection to apoptosis. Fitness costs of virulence alleles. Diversifying selection in NIP1. Week 13 Boom and bust cycles for resistance genes and fungicides and coevolutionary processes. Pathogen genetic structure and evolutionary potential. Genetic structure of pathogen populations in agroecosystems, risk assessment for pathogen evolution and breeding strategies for durable resistance. Week 14 Resistance gene and fungicide deployment strategies for agroecosystems. Week 15 Genetic engineering approaches to achieve disease resistant crops. | |||||||||||||||||||||||||||||||||||||||||
| Skript | Lecture notes will be available for purchase at the cost of reproduction. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Lecture notes will be available for purchase at the cost of reproduction. | |||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Plant Pathology I provides a good preparation for Plant Pathology II, but is not a prerequisite for this course. | |||||||||||||||||||||||||||||||||||||||||
| 551-1700-00L | Introduction to Flow Cytometry | W | 2 KP | 1V | F. Mair, M. Kisielow, M. Kopf | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This course provides a broad introduction to flow cytometry. We will cover the working principles of cytometry instrumentation, and essential aspects of experimental design and data analysis. Fluorescence-based cytometry (conventional and full spectrum) as well as mass cytometry will be covered. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | The objective of this course is to provide participants with a strong understanding of the basic principles underlying flow cytometry, equipping them to be self-sufficient for the systematic design, execution and analysis of cytometry experiments. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | The course is centered on explaining the working principles of cytometry instrumentation (conventional, full spectrum and mass cytometry) and associated data analysis. In detail, we will cover the following topics: - Components of Flow Cytometry Instrumentation - Signal processing - Fluorochromes, compensation, and spectral unmixing - Visualization, gating and analysis of data - Fluorescent panel design - Cytometry assays: immunophenotyping, cell signaling, etc. - Cell sorting - Mass cytometry - Computational data analysis techniques | |||||||||||||||||||||||||||||||||||||||||
| Skript | Updated handouts will be provided during the class. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | The lecture will reference key literature on cytometry instrumentation, experimental design, immunophenotyping and other applications. For general reading, we recommend the following: - Maciorowski et al, Basic Multicolor Flow Cytomety, Current Protocols in Immunology 2017: https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpim.26 - Cossarizza et al, Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), European Journal of Immunology 2021: https://onlinelibrary.wiley.com/doi/10.1002/eji.202170126 - Liechti et al, An updated guide for the perplexed: cytometry in the high-dimensional era, Nature Immunology 2021: https://www.nature.com/articles/s41590-021-01006-z | |||||||||||||||||||||||||||||||||||||||||
| 751-4805-00L | Recent Advances in Biocommunication | W | 3 KP | 3S | C. De Moraes | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Students will gain insight into the role of sensory cues and signals in mediating interactions within and between species. There will be a primary, but not exclusive, focus on chemical signaling in interactions among plants, insects and microbes. The course will focus on the discussion of current literature addressing key conceptual questions and state-of-the-art research techniques and methods. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | Students will gain insight into the role of sensory cues and signals in mediating interactions within and between species. There will be a primary, but not exclusive, focus on chemical signaling in interactions among plants, insects and microbes. The course will focus on the discussion of current literature addressing key conceptual questions and state-of-the-art research techniques and methods. Students will engage in discussion and critical analyses of relevant papers and present their evaluations in a seminar setting. | |||||||||||||||||||||||||||||||||||||||||
| 551-1103-00L | Microbial Biochemistry | W | 4 KP | 2V | J. Vorholt-Zambelli, J. Piel | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | The lecture course aims at providing an advanced understanding of the physiology and metabolism of microorganisms. Emphasis is on processes that are specific to bacteria and archaea and that contribute to the widespread occurrence of prokaryotes. Applied aspects of microbial biochemistry will be pointed out as well as research fields of current scientific interest. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | The lecture course aims at providing an advanced understanding of the physiology and metabolism of microorganisms. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Important biochemical processes specific to bacteria and archaea will be presented that contribute to the widespread occurrence of prokaryotes. Applied aspects of microbial biochemistry will be pointed out as well as research fields of current scientific interest. Emphasis is on concepts of energy generation and assimilation. List of topics: Microbial Biochemistry and origin of life Methanogenesis and methylotrophy Anaerobic oxidation of methane Microbial autotrophy Complex: (Ligno-)Cellulose and in demand for bioenergy Challenging: Aromatics and hydrocarbons Living on a diet and the anaplerotic provocation 20 amino acids: the making of Extending the genetic code The 21st and 22nd amino acid Some exotic biochemistry: nucleotides, cofactors Ancient biochemistry? Iron-sulfur clusters, polymers Secondary metabolites: playground of evolution | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Will be provided during the course. | |||||||||||||||||||||||||||||||||||||||||
| 529-0059-00L | Nanoscale Molecular Imaging | W | 3 KP | 2G | N. Kumar, R. Zenobi | |||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This course will provide fundamental knowledge about the principal analytical techniques for nanoscale molecular imaging. In addition to the basic concepts, students will also learn the application of advanced molecular characterization tools to solve problems in the chemical, biological and material sciences. | |||||||||||||||||||||||||||||||||||||||||
| Lernziel | This course will provide fundamental knowledge about the principal analytical techniques for nanoscale molecular imaging. In addition to the basic concepts, students will also learn the application of advanced molecular characterization tools to solve problems in the chemical, biological and material sciences. | |||||||||||||||||||||||||||||||||||||||||
| Inhalt | Nanoscale molecular imaging using fluorescence spectroscopy: - Stimulated emission depletion microscopy (STED) - Saturated structured illumination microscopy (SSIM) - Direct stochastic optical reconstruction microscopy (dSTORM) - Photoactivated localization microscopy (PALM) Nanoscale molecular imaging using Raman spectroscopy: - Scanning near-field optical microscopy (aperture SNOM) - Tip-enhanced Raman spectroscopy (TERS): Based on atomic force microscopy (AFM) & scanning tunnelling microscopy (STM) Nanoscale molecular imaging using infra-red (IR) spectroscopy: - Nanoscale Fourier-transform infrared spectroscopy (Nano-FTIR) - Photo-induced force microscopy (AFM-IR) Nanoscale molecular imaging using ions: - Nanoscale secondary ion mass spectrometry (NanoSIMS) Single molecule imaging techniques: - Scanning probe microscopy: STM & AFM - Ultrahigh vacuum (UHV)-TERS - Cryogenic electron microscopy (Cryo-EM) | |||||||||||||||||||||||||||||||||||||||||
| Skript | Lecture notes will be made available online. | |||||||||||||||||||||||||||||||||||||||||
| Literatur | Information about relevant literature will be available in the lecture & in the lecture notes. | |||||||||||||||||||||||||||||||||||||||||
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