551-1129-00L  Understanding and Engineering Microbial Metabolism

SemesterAutumn Semester 2022
LecturersJ. Vorholt-Zambelli
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 6.
The enrolment is done by the D-BIOL study administration.

AbstractThis laboratory course has a focus on current research topics in our laboratory related to metabolic engineering, the general understanding of metabolism, and is partially focused on one carbon metabolism. Projects will be conducted in small groups.
ObjectiveThe course aims at introducing technologies to investigate bacterial metabolism and key principles of metabolic engineering. The main focus of this block course is on practical work and will familiarize participants with complementary approaches, in particular genetic, biochemical and analytical techniques including metabolomics. Results will be presented by students in scientific presentations. Another goal is to learn how to write a scientific report.
ContentThe course and will include topics such as pathway elucidation & engineering and related ongoing research projects in the lab. Experimental work applied during the course will comprise methods such as cloning work & transformation, growth determination, enzyme activity assays, liquid-chromatography mass-spectrometry and dynamic labeling experiments.
Lecture notesNone
LiteratureWill be provided at the beginning of the course.