529-0058-AAL  Analytical Chemistry II

SemesterAutumn Semester 2022
LecturersD. Günther, M.‑O. Ebert, G. Schwarz, R. Zenobi
Periodicityevery semester recurring course
Language of instructionEnglish
CommentEnrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement.

Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit.

This course does not offer a lecture of its own but it is linked to the course 529-0058-00L.

AbstractEnhanced knowledge about the elemental analysis and spectrocopical techniques with close relation to practical applications. This course is based on the knowledge from analytical chemistry I. Separation methods are included.
ObjectiveUse and applications of the elemental analysis and spectroscopical knowledge to solve relevant analytical problems.
ContentCombined application of spectroscopic methods for structure determination, and practical application of element analysis. More complex NMR methods: recording techniques, application of exchange phenomena, double resonance, spin-lattice relaxation, nuclear Overhauser effect, applications of experimental 2d and multipulse NMR spectroscopy, shift reagents. Application of chromatographic and electrophoretic separation methods: basics, working technique, quality assessment of a separation method, van-Deemter equation, gas chromatography, liquid chromatography (HPLC, ion chromatography, gel permeation, packing materials, gradient elution, retention index), electrophoresis, electroosmotic flow, zone electrophoresis, capillary electrophoresis, isoelectrical focussing, electrochromatography, 2d gel electrophoresis, SDS-PAGE, field flow fractionation, enhanced knowledge in atomic absorption spectroscopy, atomic emission spectroscopy, X-ray fluorescence spectroscopy, ICP-OES, ICP-MS.
Literaturegeneral: R. Kellner, J.-M. Mermet, M. Otto, H. M. Widmer (Eds.) Analytical Chemistry, Wiley-VCH, Weinheim, 1998;
XRF: R. Schramm, X-Ray Fluorescence Analysis: Practical and Easy, Fluxana, Kleve, 2012;
ICP-MS: R. Thomas, Practical Guide to ICP-MS - A Tutorial for beginners, 3rd Edition, CRC Press, Taylor & Francis Group, Boca Raton, 2013 (especially: chapters 1-15, 19 and 21).
Separation methods: S. Ahuja (Ed.), Chromatography and Separation Science, Volume 4 of series "Separation Science and Technology", Elsevier Academic Press, San Diego, 2003.
K. Robards, P. R. Haddad, and P. E. Jackson, Principle and Practise of Modern Chromatographic Methods, Academic Press, London, 1994.
F. Foret, L. Krivankova, and P. Bocek, Capillary Zone Electrophoresis, VCH, Weinheim (1993)
Prerequisites / NoticeNone.