551-0128-00L  Fundamentals of Biology I

SemesterSpring Semester 2022
LecturersM. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler
Periodicityyearly recurring course
Language of instructionGerman
CommentRegistrations via myStudies until 28.1.2022 at the latest. Subsequent registrations will not be considered.


AbstractThis 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concepts and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days.
ObjectiveIntroduction to the biology of unicellular organisms and practical experience with biochemical and molecular biology techniques.

General Praktikum-information and course material can be obtained from Moodle

The general Praktikum information (Assignment list, Instructions and Schedule & Performance Sheet) will also be sent to the students directly (E-mail).
ContentThis 1st year Laboratory course introduces the students to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days. The practical will operate in two shifts on Thursday and Friday.

morning shift 8:00-13:00

afternoon shift 13:30-18:30

Program

Day 1: Handling and detection of microorganisms
Day 2: Morphological, biochemical and genetic differentiation of microorganisms
Day 3: Biotic interactions and genetics of bacteria
Day 4: Metabolism and physiology of bacteria
Day 5: DNA extraction, tranformation (E.coli, yeast)
Day 6: RNA extraction, Life cycle of eukaryotic cells
Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography
Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB)
Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase
Day 10: Affinity chromatography, protein crystallization and structure
Day 11: Protein folding, protein stability and Enzyme kinetics
Day 12: Protein folding, protein stability and Enzyme kinetics
Lecture notesLaboratory manuals can be downloaded from Moodle
Prerequisites / NoticePLEASE NOTE THE FOLLOWING RULES

Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day.

If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not.

HIGHLY IMPORTANT!!

1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS21 but not later than January 28, 2022.

2. Later registration is NOT possible and can NOT be accepted!

3. The course registration for FS2022 is usually possible at the end of fall semester 2021 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible.

The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2022. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester.

PRAKTIKUM DAYS FS22 (Thursdays):

24.02.; 03.03.; 10.03.; 17.03.; 24.03.; 31.03.; 07.04.; 28.04.; 5.05.; 12.05.; 19.05.; 02.06.

PRAKTIKUM DAYS FS22 (Fridays):

25.02.; 04.03.; 11.03.; 18.03.; 25.03.; 1.04.; 8.04.; 29.04.; 06.05.; 13.05.; 20.05.; 03.06.

No Praktikum during the Easter vacation: 11.04-22.04.