551-0128-00L Fundamentals of Biology I
|Semester||Spring Semester 2021|
|Lecturers||M. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler|
|Periodicity||yearly recurring course|
|Language of instruction||German|
|Comment||Registrations via myStudies until 29.1.2021 at the latest. Subsequent registrations will not be considered. |
General safety regulations:
-Whenever possible the distance rules have to be respected.
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged.
-Any additional rules for individual courses have to be respected.
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible.
|Abstract||This 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concept and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days.|
|Objective||Introduction to biology with practical work.|
General Praktikum-information and course material can be obtained from Moodle
The general Praktikum information (Assignment list, Instructions and Schedule & Performance Sheet) will also be sent to the students directly (E-mail).
|Content||This 1st year Laboratory course introduces the student to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days.|
Because of necessary Covid-19 safety measures the practical will operate in two shifts to reduce the number of student in the practical rooms.
1. shift 8:00-13:00
2. shift 13:30-18:30
Day 1: Isolation and cultivation of microorganisms
Day 2: Morphological, biochemical and genetic characterization of microorganisms
Day 3: Evolution of microorganisms and their abiotic and biotic interactions
Day 4: Lifestyle of fungi as eukaryotic representatives of the microorganisms
Day 5: DNA extraction, tranformation (E.coli, yeast)
Day 6: RNA extraction, Life cycle of eukaryotic cells
Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography
Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB)
Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase
Day 10: Affinity chromatography, protein crystallization and structure
Day 11: Protein folding, protein stability and Enzyme kinetics
Day 12: Protein folding, protein stability and Enzyme kinetics
|Lecture notes||Laboratory manuals can be downloaded from Moodle|
|Prerequisites / Notice||PLEASE NOTE THE FOLLOWING RULES|
Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day.
If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not.
1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS20 but not later than January 29, 2021.
2. Later registration is NOT possible and can NOT be accepted!
3. The course registration for FS2020 is usually possible at the end of fall semester 2020 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible.
The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2021. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester.
Necessary changes in the course program in response to enhanced COVID-19 safety measures are possible and will be communicated via email and moodle.
PRAKTIKUM DAYS FS21 (Thursdays):
25.02.; 04.03.; 11.03.; 18.03.; 25.03.; 15.04.; 22.04.; 29.04.; 06.05.; 20.05.; 27.05.; 03.06.
PRAKTIKUM DAYS FS21 (Fridays):
26.02.; 05.03.; 12.03.; 19.03.; 26.03.; 16.04.; 23.04.; 30.04.; 07.05.; 21.05.; 28.05.; 04.06.
No Praktikum during the Easter vacation: 2.04.-9.04