636-0205-00L Lab Course: Mammalian Gene Circuits
Semester | Spring Semester 2021 |
Lecturers | Y. Benenson |
Periodicity | yearly recurring course |
Language of instruction | English |
Courses
Number | Title | Hours | Lecturers | |
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636-0205-00 P | Lab Course: Mammalian Gene Circuits
![]() This Lab Course will take place on the following dates: Mon/Tue March 15/16 Mon/Tue March 22/23 Mon/Tue March 29/30 Mon/Tue April 12/13 Mon/Tue April 19/20 Attention: Mon/Tue April 5/6 – no lab course: Easter Break (April 2 – April 11) | 64s hrs | Y. Benenson |
Catalogue data
Abstract | The students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level. |
Learning objective | The objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization. |
Content | The course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1. Week 2: Cloning of the constructs, purification and characterization of DNA constructs Week 3: Cell culture transfection, microscopy and flow cytometry characterization Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments. |
Lecture notes | Preparatory materials will be provided before the start of the course. |
Literature | Will be provided before the course |
Performance assessment
Performance assessment information (valid until the course unit is held again) | |
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ECTS credits | 2 credits |
Examiners | Y. Benenson |
Type | graded semester performance |
Language of examination | English |
Repetition | Repetition only possible after re-enrolling for the course unit. |
Additional information on mode of examination | Written lab report required |
Learning materials
No public learning materials available. | |
Only public learning materials are listed. |
Groups
No information on groups available. |
Restrictions
General | ![]() |
Offered in
Programme | Section | Type | |
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Biotechnology Master | Practical Training | O | ![]() |