636-0207-00L  Lab Course: Cellular Engineering Stem Cells

SemesterSpring Semester 2021
LecturersT. Schroeder
Periodicityyearly recurring course
Language of instructionEnglish
CommentAttention: This lab course was offered in previous semesters with the number: 626-0806-00L "Laboratory Course Stem Cell Purification, Culture and Manipulation”. Students that already passed course 626-0806-00L cannot receive credits for course 636-0207-00L.



Courses

NumberTitleHoursLecturers
636-0207-00 PLab Course: Cellular Engineering Stem Cells Special students and auditors need a special permission from the lecturers.
This lab course will take place after the end-of-semester-exam-period of spring semester and the Cellular Engineering Mammalian Cells lab course:
Monday, July 5 - Friday, July 16, 10 full days
80s hrsT. Schroeder

Catalogue data

AbstractMammalian stem cells of different organs are purified, cultured, differentiated, analyzed and manipulated. Plasmids and viral vectors will be cloned, produced and transfected / transduced to manipulate stem cells. Computational and analytical molecular biology methods, FACS and imaging and lectures complement the program.
ObjectiveIndependent planning and conducting of experiments with mammalian stem cells including all steps from culturing different cell lines to DNA transfection / transduction and expression analysis by different analytical methods. Documenting and writing a report on conducted experiments and results.
ContentPractical course on purification of primary mammalian stem cells, culture of primary stem cells and stem cell lines, characterization, manipulation and differentiation of stem cells. Construction of plasmids or viral vectors for gene expression, DNA transfer by transfection and transduction, analysis of gene expression by fluorescent proteins, PCR, fluorescence-activated cell sorting (FACS), imaging. Documentation of experiments in a laboratory journal, writing of a report on the experiments and results.

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits2 credits
ExaminersT. Schroeder
Typeungraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.
Additional information on mode of examinationGroup examination (colloquium) of 3 students at the end of the course, 20 Minutes per group.
Written report on experiments and results will be graded as pass/fail and is required to pass the course.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

General : Special students and auditors need a special permission from the lecturers

Offered in

ProgrammeSectionType
Biotechnology MasterPractical TrainingOInformation