551-0352-00L  Introduction to Mass Spectrometry-Based Proteomics

SemesterSpring Semester 2021
LecturersL. Gillet, P. Picotti
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 12.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible



Courses

NumberTitleHoursLecturers
551-0352-00 PIntroduction to Mass Spectrometry-Based Proteomics
Permission from lecturers required for all students.
Block course in the 2nd quarter of the spring semester
100s hrs
Tue/107:45-16:30HIT F 32 »
Wed/107:45-16:30HIT F 32 »
Thu/107:45-16:30HIT F 32 »
Fri/107:45-16:30HIT F 32 »
L. Gillet, P. Picotti

Catalogue data

AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
ObjectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits6 credits
ExaminersL. Gillet, P. Picotti
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

GeneralPermission from lecturers required for all students

Offered in

ProgrammeSectionType
Biology BachelorBlock Courses in 2nd Quarter of the SemesterWInformation