535-0310-00L  Glycobiology in Drug Development

SemesterAutumn Semester 2020
LecturersV. I. Otto
Periodicityyearly recurring course
Language of instructionEnglish

AbstractProtein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples of prominent glycoprotein drugs, the course aims at providing insight into glycosylation-activity relationships and into biotechnological production and analytics.
ObjectiveStudents gain basic knowledge in "pharmaceutical glycobiology" This implies knowing and understanding:
- major mechanisms underlying the roles of glycosylation for the biological/therapeutic actions of glycoproteins (glycosylation-function relationships) using prominent examples of glycoprotein drugs.
- the major types of protein-linked glycans and the biosynthetic pathways for their formation
- how glycoprotein drugs are produced (including the most important expression systems used), glycoengineered and analysed (quality control)
Students are able to apply this knowledge in solving simple problems in glycoprotein drug development (on paper).
Students gain the ability to reflect on roles of glycosylation in various biological contexts.
Contentlecture plan:
1. Glycans - information carriers in biology and pharmacotherapy
2. Glucocerebrosidase and the biosynthesis of N-glycans
3. Glyocerebrosidase - production and quality control
4. Improving the therapeutic profile of monoclonal antibodies by glycoengineering
5. Mucin-type O-glycans and sialyltion as gCQA of glycoprotein hormones drugs - production and quality control.
6. EPO "the same but different"
The lectures will include some exercises in which students apply their knowledge to solve simple biotechnological problems related to protein glycosylation.
Lecture notesThe slides used for the lectures will be provided online
Literature- Essentials of Glycobiology 3rd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2017.
- recent publications as cited/proposed on the lecture slides
Prerequisites / NoticeRequirements: Basic knowledge in immunology, molecular biology, protein and carbohydrate chemistry, analytical techniques. Basic knowledge in pharmacology.