535-0810-00L Gene Technology
| Semester | Herbstsemester 2020 |
| Dozierende | K. Eyer, J. Scheuermann |
| Periodizität | jährlich wiederkehrende Veranstaltung |
| Lehrsprache | Englisch |
| Kurzbeschreibung | The course gives a description and summary of the field of gene technology and its pharmaceutical applications. The course focuses on important methods and technologies and their application for genomic, transcriptomic and proteomic analyses in human biology. |
| Lernziel | The course gives an overview of current state-of-the art and advancement in the fields of gene technology. Herein, the course focuses on genomic, transcriptomic and proteomic analysis and their uses in drug discovery and biomedical applications. The course is structured into lectures and practical examples drawn from the research field. Upon completion, the students are familiar and know current state-of-the art of methods and applications, but are also able to classify, contrast and apply different strategies and methods within the field of gene technology. The course is suited for advanced undergraduate and early graduate students in pharmaceutical sciences or related fields. |
| Inhalt | I) Genomics and transcriptomics Methods and Techniques: • Recombinant DNA technology • Next generation sequencing methods, sequencing of genomes • CRISPR technology Application to human biology: • Functional genomics/transcriptomics • Principles of cancer, genetic diseases • Therapies: cell-based therapies/gene therapies/DNA and RNA vaccination II) Proteomics Methods and Techniques: • Protein cloning and expression • The antibody molecule • Measurement and determination of biomolecular interactions • Protein characterization and engineering • Modifications and radioactive labelling Application to human biology: • Protein therapeutics • Proteomic approaches for identification of novel disease-related targets and biomarkers III) Drug discovery: Protein-based libraries •Immune repertoire mining •Display and selection technologies 1. antibody phage display 2. other polypeptide display technologies 3. small-molecules display: DNA-encoded chemical libraries |