636-0104-00L  Biophysical Methods

SemesterHerbstsemester 2020
DozierendeD. J. Müller
Periodizitätjährlich wiederkehrende Veranstaltung
KommentarAttention: This course was offered in previous semesters with the number: 626-0010-00L "Nanomachines of the Cell (Part I): Principles". Students that already passed course 626-0010-00 cannot receive credits for course 636-0104-00.


636-0104-00 GBiophysical Methods
Attention: Lecture starts on Wednesday, September 23
The lecturers will communicate the exact lesson times of ONLINE courses.
3 Std.
Mi10:00-13:00ON LI NE »
D. J. Müller


KurzbeschreibungStudents will be imparted knowledge in basic and advanced biophysical methods applied to problems in molecular biotechnology. The course is fundamental to applying the methods in their daily and advanced research routines. The students will learn the physical basis of the methods as well as their limitations and possibilities to address existing and future topics in molecular biotechnology.
LernzielGain of interdisciplinary competence in experimental and theoretical research, which qualifies for academic scientific work (master's or doctoral thesis) as well as for research in a biotechnology or a pharmaceutical company. The module is of general use in courses focused on modern biomolecular technologies, systems biology and systems engineering.
InhaltThe students will learn basic and advanced knowledge in applying biophysical methods to address problems and overcome challenges in biotechnology, cell biology and life sciences in general. The biological and physical possibilities and limitations of the methods will be discussed and critically evaluated. By the end of the course the students will have assimilated knowledge on a portfolio of biophysical tools widening their research capabilities and aptitude.
The biophysical methods to be taught will include:
• Light microscopy: Resolution limit of light microscopy, fluorescence, GFP, fluorescence microscopy, DIC, phase contrast, difference between wide-field and confocal microscopy
• Super resolution optical microscopy: STED, PALM, STORM, other variations
• Electron microscopy: Scanning electron microscopy, transmission electron microscopy, electron tomography, cryo-electron microscopy, single particle analysis and averaging, tomography, sectioning, negative stain
• X-ray, electron and neutron diffraction
• MRI Imaging
• Scanning tunnelling microscopy and atomic force microscopy
• Patch clamp technologies: Principles of patch clamp analysis and application. Various patch clamp approaches used in research and industry
• Surface plasmon resonance-based biosensors
• Molecular pore-based sensors and sequencing devices
• Mechanical molecular and cellular assembly devices
• Optical and magnetic tweezers
• CD spectroscopy
• Optogenetics
• Molecular dynamics simulations
SkriptHand out will be given to students at lecture.
LiteraturMethods in Molecular Biophysics (5th edition), Serdyuk et al., Cambridge University Press
Biochemistry (5th edition), Berg, Tymoczko, Stryer; ISBN 0-7167-4684-0, Freeman
Bioanalytics, Lottspeich & Engels, Wiley VCH, ISBN-10: 3527339191
Cell Biology, Pollard & Earnshaw; ISBN:0-7216-3997-6, Saunder, Pennsylvania
Methods in Modern Biophysics, Nölting, 3rd Edition, Springer, ISBN-10: 3642030211
Voraussetzungen / BesonderesThe module is composed of 3 SWS (3 hours/week): 2-hour lecture, 1-hour seminar. For the seminar, students will prepare oral presentations on specific in-depth subjects with/under the guidance of the teacher.


Information zur Leistungskontrolle (gültig bis die Lerneinheit neu gelesen wird)
Leistungskontrolle als Semesterkurs
ECTS Kreditpunkte4 KP
PrüfendeD. J. Müller
RepetitionDie Leistungskontrolle wird in jeder Session angeboten. Die Repetition ist ohne erneute Belegung der Lerneinheit möglich.
Prüfungsmodusmündlich 20 Minuten
Zusatzinformation zum PrüfungsmodusOral presentation in class amounts to 25% and written report to 25% of final grade. Both are continuous compulsary performance assessments.
Diese Angaben können noch zu Semesterbeginn aktualisiert werden; verbindlich sind die Angaben auf dem Prüfungsplan.


Keine öffentlichen Lernmaterialien verfügbar.
Es werden nur die öffentlichen Lernmaterialien aufgeführt.


Keine Informationen zu Gruppen vorhanden.


Keine zusätzlichen Belegungseinschränkungen vorhanden.

Angeboten in

Biotechnologie MasterBiomolekulare OrientierungWInformation
Biotechnologie MasterSystem-OrientierungWInformation
Computational Biology and Bioinformatics MasterBiophysicsWInformation