701-1425-01L Genetic Diversity: Techniques
| Semester | Autumn Semester 2020 |
| Lecturers | A. M. Minder Pfyl |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 8. Selection of the students: order of registration Registration until 18.10.2020. General safety regulations for all this course: -Whenever possible the distance rules have to be respected -All students have to wear masks throughout the course (keep reserve masks ready) -The installation and activation of the Swiss Covid-App is highly encouraged -Any additional rules for individual courses have to be respected -Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible |
Courses
| Number | Title | Hours | Lecturers | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 701-1425-01 P | Genetic Diversity: Techniques Language of the course: English Start of the course: Wednesday, 4. November 2020 at 13:15-17:00, end of the course: Wednesday, 25. November 2020 at 13:15-17:00, individual work in between (about 1 day per week preferably Monday to Wednesday). Course room for the introduction and final discussion will be announced, lab work has to be done at the GDC. | 60s hrs |
| A. M. Minder Pfyl |
Catalogue data
| Abstract | This course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction protocols, quality control measurements, SNP genotyping and gene expression techniques will be addressed. |
| Learning objective | To learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing etc. A course for practicioners. |
| Content | After an introduction (one afternoon), students will have 3 weeks to work independently in groups of two through different protocols. At the end the whole class meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques. Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR. |
| Lecture notes | Material will be handed out in the course. |
| Literature | Material will be handed out in the course. |
| Prerequisites / Notice | Two afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student. |
Performance assessment
| Performance assessment information (valid until the course unit is held again) | |
Performance assessment as a semester course | |
| ECTS credits | 2 credits |
| Examiners | A. M. Minder Pfyl |
| Type | ungraded semester performance |
| Language of examination | English |
| Repetition | Repetition only possible after re-enrolling for the course unit. |
| Additional information on mode of examination | Attendance and active participation in workshops. Final talk. |
Learning materials
| No public learning materials available. | |
| Only public learning materials are listed. |
Groups
| No information on groups available. |
Restrictions
| Places | 8 at the most |
| Waiting list | until 26.10.2020 |
| End of registration period | Registration only possible until 12.10.2020 |
Offered in
| Programme | Section | Type | |
|---|---|---|---|
| Doctoral Department of Environmental Sciences | Ecology and Evolution | W |  |
| Environmental Sciences Master | Laboratory and Field Expertise | W | |