551-1554-00L  Multigene Expression in Mammalian Cells

SemesterSpring Semester 2020
LecturersP. Berger, G. Schertler
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 5.

The enrolment is done by the D-BIOL study administration.



Courses

NumberTitleHoursLecturers
551-1554-00 GMultigene Expression in Mammalian Cells
Permission from lecturers required for all students.
Block course in the 2nd quarter of the spring semester
Place: Paul Scherrer Institut
100s hrsP. Berger, G. Schertler

Catalogue data

AbstractGenetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.
ObjectiveStudents will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.
ContentWe will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.
Lecture notesnone

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits6 credits
ExaminersG. Schertler, P. Berger
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.
Additional information on mode of examinationAssessment during the course, Presentation, Written report (1/3 each), German/English

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

GeneralPermission from lecturers required for all students

Offered in

ProgrammeSectionType
Biology BachelorBlock Courses in 2nd Quarter of the SemesterWInformation