551-1415-00L Image-based Drug Screening in Human Blood for Personalized Medicine
| Semester | Autumn Semester 2019 |
| Lecturers | B. Snijder, further lecturers |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 5. The enrolment is done by the D-BIOL study administration. |
| Abstract | Image based screening allows to measure in high throughput the phenotype of millions of individual cells to external perturbations. We have recently shown that image-based screening in human blood can help to find active treatments for patients with blood cancers. In this course we will take the students through the entire workflow (to the extent that biosafety regulations allow it). |
| Learning objective | Take the students through the entire workflow from experimental design, to screen, to imaging and analysis. -Learn to design an image-based screening experiment -Observe human blood sample handling -Perform immunofluorescence & automated confocal microscopy -Image analysis and result interpretation -Result presentation |
| Literature | -Relevant study: https://www.thelancet.com/journals/lanhae/article/PIIS2352-3026(17)30208-9/fulltext -Editorial commentary: https://www.thelancet.com/journals/lanhae/article/PIIS2352-3026(17)30213-2/fulltext |