529-0837-00L  Biomicrofluidic Engineering

SemesterHerbstsemester 2019
DozierendeA. de Mello
Periodizitätjährlich wiederkehrende Veranstaltung
KommentarNumber of participants limited to 5.

Only for Chemical and Bioengineering MSc, Programme Regulations 2005.

IMPORTANT NOTICE for Chemical and Bioengineering students: There are two different version of this course for the two regulations (2005/2018), please make sure to register for the correct version according to the regulations you are enrolled in.

KurzbeschreibungMicrofluidics describes the behaviour, control and manipulation of fluids that are geometrically constrained within sub-microliter environments. The use of microfluidic devices offers an opportunity to control physical and chemical processes with unrivalled precision, and in turn provides a route to performing chemistry and biology in an ultra-fast and high-efficiency manner.
LernzielThe course will present the theoretical concepts behind the operation and functioning of microfluidic systems, the methods of microfluidic device manufacture and the application of microfluidic architectures and tools to important problems faced in modern day chemical and biological analysis. A key feature of the course will be a research project. The project will run from mid October until mid December. The aim of the project is to develop an understanding of the process of microfluidic design and how microfluidic tools can be applied to chemical or biological problems. The project will involve literature analysis, CFD simulations and experimental work. Students will be expected to present their results through a paper and class presentation.

In general the course will: Introduce the key phenomena that dictate how fluids behave when contained within small volume systems; Explain why the miniaturisation of basic laboratory instrumentation leads to significant gains in experimental “performance”; Present the structure, operation and performance of key microfluidic components; Showcase how microfluidic tools have been used to address important problems in chemistry and biology; Allow students to use this knowledge to design microfluidic tools for specific chemical/biological applications.
InhaltSpecific topics that will be addressed during the course include:

Theoretical Concepts: Scaling laws, features of thermal/mass transport, diffusion, basic description of fluid flow in small volumes, microfluidic mixing strategies • Microfluidic Device Manufacture: Basic principles of conventional lithography of rigid materials, ‘soft’ lithography, polymer machining (injection molding, hot embossing and 3D printing) • Analytical Separations: Principles of electrophoresis, electroosmosis, high performance capillary electrophoresis, scaling laws, chip-based electrophoresis and isoelectric focusing • Heat & Mass Transfer: Heat transfer, mass transfer, unit operations, dimensionless numbers, scaling laws, continuous and segmented flows • Computational Fluid Dynamics: Introduction to COMSOL, essentials of microfluidic modelling, application to microfluidic problems • DNA Analysis: Amplification and analysis of nucleic acids on the microscale, oligonucleotide microarrays for high-throughput sequence analysis • Droplet-based Microfluidics: Principles behind the formation, manipulation and use of liquid/liquid segmented flows in high-throughput experimentation • Small Volume Analysis: Application of optical methods for high-throughput and high-content detection in sub-nL volumes • Cellular Analysis: Application of microfluidic tools for high-throughput cell-based analysis, flow cytometry and single cell analysis.
SkriptLecture handouts, background literature, problem sheets and notes will be made accessible to enrolled students through the lecture Moodle site.
LiteraturThere is no textbook associated with the course. However, the following articles provide useful background reading prior to enrolment:

1. The origins and the future of microfluidics; G.M. Whitesides, Nature, 442, 368–373 (2006)
2. Control and detection of chemical reactions in microfluidic systems; A.J. deMello, Nature, 442, 394–402 (2006)
3. Small but Perfectly Formed? Successes, Challenges, and Opportunities for Microfluidics in the Chemical and Biological Sciences; D.T. Chiu, A.J. deMello, D. Di Carlo, P.S. Doyle, C. Hansen, R.M. Maceiczyk, R.C.R. Wootton, Chem, 2, 201-223 (2017)