636-0206-00L  Lab Course: Cellular Engineering Mammalian Cells

SemesterSpring Semester 2019
LecturersM. Fussenegger, A. M. Palma Teixeira
Periodicityyearly recurring course
Language of instructionEnglish
CommentOnly for Biotechnology MSc, Programme Regulations 2017
Attention: This lab course was offered in previous semesters with the number: 626-0802-00L "Practical Course in Mammalian Cell Biotechnology”. Students that already passed course 626-0802-00L cannot receive credits for course 636-0206-00L.


AbstractMammalian cells will be transfected and transduced for the production of biopharmaceuticals, for drug discovery as well as for the design of synthetic biology-inspired programmable gene circuits. A wide array of analytical techniques, lectures, and excursions to biotech companies will complement the practical part.
ObjectiveIndependent planning and conducting of experiments with mammalian cells including all steps from culturing different cell lines to DNA transfection/transduction and expression analysis using a wide array of analytical methods.
ContentA practical course on characterization and cultivation of mammalian cells, DNA transfer by transfection, construction of synthetic gene networks, analysis of gene expression by enzymatic and immunological methods and fluorescent proteins, bioprocessing, mammalian cell-based assays for drug discovery and diagnostics. Excursions to Biotech/Pharma companies.
Lecture notesWill be distributed on first day of the practical course