636-0206-00L  Lab Course: Cellular Engineering Mammalian Cells

SemesterFrühjahrssemester 2019
DozierendeM. Fussenegger, A. M. Palma Teixeira
Periodizitätjährlich wiederkehrende Veranstaltung
LehrspracheEnglisch
KommentarOnly for Biotechnology MSc, Programme Regulations 2017
Attention: This lab course was offered in previous semesters with the number: 626-0802-00L "Practical Course in Mammalian Cell Biotechnology”. Students that already passed course 626-0802-00L cannot receive credits for course 636-0206-00L.


KurzbeschreibungMammalian cells will be transfected and transduced for the production of biopharmaceuticals, for drug discovery as well as for the design of synthetic biology-inspired programmable gene circuits. A wide array of analytical techniques, lectures, and excursions to biotech companies will complement the practical part.
LernzielIndependent planning and conducting of experiments with mammalian cells including all steps from culturing different cell lines to DNA transfection/transduction and expression analysis using a wide array of analytical methods.
InhaltA practical course on characterization and cultivation of mammalian cells, DNA transfer by transfection, construction of synthetic gene networks, analysis of gene expression by enzymatic and immunological methods and fluorescent proteins, bioprocessing, mammalian cell-based assays for drug discovery and diagnostics. Excursions to Biotech/Pharma companies.
SkriptWill be distributed on first day of the practical course