551-0438-00L  Protein Folding, Assembly and Degradation

SemesterFrühjahrssemester 2019
DozierendeR. Glockshuber, E. Weber-Ban
Periodizitätjedes Semester wiederkehrende Veranstaltung
LehrspracheEnglisch
KommentarNumber of participants limited to 6.

The enrolment is done by the D-BIOL study administration.



Lehrveranstaltungen

NummerTitelUmfangDozierende
551-0438-00 GProtein Folding, Assembly and Degradation
Bewilligung der Dozierenden für alle Studierenden notwendig.
Block course in the semester break.
100s Std.R. Glockshuber, E. Weber-Ban

Katalogdaten

KurzbeschreibungStudents will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese.
LernzielThe course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner.
InhaltThe students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work.

Participation in one of the following projects will be possible:

Projects of the Glockshuber group:
- Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria.
- Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits.
- Identification of intermediates in the aggregation of the human Abeta peptide

Experimental work on these projects involves
- Molecular cloning, recombinant protein production in E. coli and protein purification
- Protein crystallization
- Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy
- Analysis of rapid reactions by stopped-flow fluorescence
- Negative-stain electron microscopy
- Light scattering



Projects of the Weber-Ban group:

- Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering
Voraussetzungen / BesonderesMarks will be given according to the following criteria:

- Planning, execution and documentation of experimental work
- Final report, including introduction with short overview on the relevant literature, results with figures and brief discussion (maximum: 5 pages)
- Performance in the exercises

Leistungskontrolle

Information zur Leistungskontrolle (gültig bis die Lerneinheit neu gelesen wird)
Leistungskontrolle als Semesterkurs
ECTS Kreditpunkte6 KP
PrüfendeR. Glockshuber
Formbenotete Semesterleistung
PrüfungsspracheEnglisch
RepetitionRepetition nur nach erneuter Belegung der Lerneinheit möglich.
Zusatzinformation zum PrüfungsmodusDie der Benotung des Blockkurses zugrundeliegenden Kriterien werden zu Beginn des Kurses bekanntgegeben. Es wird nur der hauptverantwortliche Examinator aufgeführt. Weitere Examinatoren: Die oben aufgeführten Dozenten.

Lernmaterialien

Keine öffentlichen Lernmaterialien verfügbar.
Es werden nur die öffentlichen Lernmaterialien aufgeführt.

Gruppen

Keine Informationen zu Gruppen vorhanden.

Einschränkungen

AllgemeinBewilligung der Dozierenden für alle Studierenden notwendig

Angeboten in

StudiengangBereichTyp
Biologie BachelorBlockkurse in den SemesterferienWInformation