551-0352-00L  Protein Analysis by Mass Spectrometry

SemesterSpring Semester 2019
LecturersL. Gillet, B. Collins, P. Picotti
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 12.

The enrolment is done by the D-BIOL study administration.



Courses

NumberTitleHoursLecturers
551-0352-00 GProtein Analysis by Mass Spectrometry
Permission from lecturers required for all students.
Block course in the 2nd quarter of the spring semester
(combined with block course 551-0362-00 in the 3rd quarter of the spring semester)
100s hrs
Tue12:45-16:30HIT F 32 »
Wed07:45-16:30HIT F 32 »
Thu07:45-16:30HIT F 32 »
Fri07:45-16:30HIT F 32 »
L. Gillet, B. Collins, P. Picotti

Catalogue data

AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
ObjectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits6 credits
ExaminersB. Collins, L. Gillet, P. Picotti
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

GeneralPermission from lecturers required for all students

Offered in

ProgrammeSectionType
Biology BachelorBlock Courses in 2nd Quarter of the SemesterWInformation