529-0735-01L  Chemical Aspects of Bioimaging

SemesterAutumn Semester 2018
LecturersP. Rivera Fuentes
Periodicityyearly recurring course
Language of instructionEnglish

AbstractThis course will introduce basic concepts of fluorescence spectroscopy and microscopy applied to the observation of biological systems. The course will focus on the design, preparation and implementation of small-molecule and protein-based probes for biological investigations.
ObjectiveTo understand the basic chemical aspects of bioimaging and photoactivation in biology.
ContentPrinciples of fluorescence spectroscopy and microscopy, fluorescent dyes and proteins, chemiluminescence, super-resolution microscopy, and fluorescent sensors.
Lecture notesHandouts, selected original literature, quizzes, and other materials will be provided electronically.
LiteratureJ. R. Lakowicz. Principles of Fluorescence Spectroscopy. Kluwer Academic / Plenum Publishers. 2006.

P. J. Walla. Modern Biophysical Chemistry: Detection and Analysis of Biomolecules. Wiley-VCH. 2014.

M. Chalfie; S. R. Kain (Eds.) Green Fluorescent Protein: Properties, Applications, and Protocols.
Wiley-Interscience. 2006.

R. W. Sabnis. Handbook of Fluorescent Dyes and Probes. John Wiley & Sons, Inc. 2015.

A. P. Demchenko. Introduction to Fluorescence Sensing. Springer Science. 2009.