529-0735-01L Chemical Aspects of Bioimaging
| Semester | Autumn Semester 2018 |
| Lecturers | P. Rivera Fuentes |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Abstract | This course will introduce basic concepts of fluorescence spectroscopy and microscopy applied to the observation of biological systems. The course will focus on the design, preparation and implementation of small-molecule and protein-based probes for biological investigations. |
| Learning objective | To understand the basic chemical aspects of bioimaging and photoactivation in biology. |
| Content | Principles of fluorescence spectroscopy and microscopy, fluorescent dyes and proteins, chemiluminescence, super-resolution microscopy, and fluorescent sensors. |
| Lecture notes | Handouts, selected original literature, quizzes, and other materials will be provided electronically. |
| Literature | J. R. Lakowicz. Principles of Fluorescence Spectroscopy. Kluwer Academic / Plenum Publishers. 2006. P. J. Walla. Modern Biophysical Chemistry: Detection and Analysis of Biomolecules. Wiley-VCH. 2014. M. Chalfie; S. R. Kain (Eds.) Green Fluorescent Protein: Properties, Applications, and Protocols. Wiley-Interscience. 2006. R. W. Sabnis. Handbook of Fluorescent Dyes and Probes. John Wiley & Sons, Inc. 2015. A. P. Demchenko. Introduction to Fluorescence Sensing. Springer Science. 2009. |