751-6129-00L  Practical Course Epigenetics

SemesterAutumn Semester 2018
LecturersS. E. Ulbrich, M. Saenz de Juano Ribes
Periodicityyearly recurring course
Language of instructionEnglish

AbstractThe practical course will comprise lecture elements on introducing the topic of epigenetics in detail to you and a large amount of practical work where you will be able to perform DNA methylation analyses on your own. We will focus on DNA extraction, the estimation of Global DNA Methylation and Gene-specific Methylation.
ObjectiveThe competencies and aims for the course are as follows:
Get first hands-on experience with the experimental techniques
Answer a scientific question by conducting an experiments
Present the principle of different techniques to other students
Obtain results of an experiment and get insight into what affects technical variation and thus influences reproducibility
Interpret results in an adequate manner to solve a scientific question
Combine results to draw an adequate conclusion
Present an epigenetics research paper
Lecture notesYou will receive a draft outline of the week, the slides of the theoretical parts and a detailed protocol of the work we will do in advance of the practical course.
Prerequisites / NoticeFor receiving a total of 3 Credit Points for this practical course we kindly ask you to actively take part in the practical performance. You need to pass a 30-min written examination about the theoretical background of the techniques, approaches and the background. In addition, we ask you to present an original research paper together with a colleague. You will receive the paper in advance for preparation. We will ask you to prepare a 30 min presentation with a following discussion. Next to the presentation of your scientific paper you are asked to address questions of the presentations of your colleagues and actively take part in the discussion. Finally, we will ask you to write a lab report to be handed in at the beginning of the spring semester.