551-1403-00L Imaging Bacterial Cells in a Native State by Electron Cryotomography
| Semester | Autumn Semester 2018 |
| Lecturers | M. Pilhofer |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 5. The enrolment is done by the D-BIOL study administration. |
| Abstract | The goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures |
| Learning objective | Students will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization. https://www.mol.biol.ethz.ch/groups/pilhofer_group/ |