529-0739-10L  Biological Chemistry A: Technologies for Directed Evolution of Enzymes

SemesterAutumn Semester 2018
LecturersP. A. Kast, D. Hilvert
Periodicityyearly recurring course
Language of instructionEnglish
CommentAdvanced laboratory course or internship depending on lab course Biological Chemistry B

Candidates must inquire with P. Kast no later than September 1st whether course will take place (no self-enrollment)

Further information to registration and work hours: Link



Courses

NumberTitleHoursLecturers
529-0739-10 PBiological Chemistry A: Technologies for Directed Evolution of Enzymes Special students and auditors need a special permission from the lecturers.
Permission from lecturers required for all students.
16 hrsby appt.P. A. Kast, D. Hilvert

Catalogue data

AbstractDuring this semester course, methodologies will be taught for biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains.
ObjectiveAll technologies used for the experiments will be explained to the students in practice with the goal that they will be able to independently apply them for the course project and in future research endeavors. After the course, an individual report about the results obtained has to be prepared.
ContentThis class conducts and supports experiments for a specifically designed genuine research project. We will carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. The relevant technologies will be taught to the students, such as the preparation of competent cells, production and isolation of DNA fragments, transformation of gene libraries, and DNA sequencing. The course participants will generate a variety of different variants of a chorismate mutase. Individual enzyme catalysts will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters, their molecular mass, and the integrity of the protein structure. The students will present the results obtained from their individual evolution experiments at the end of the semester. We expect that during this lab course we will not only generate novel enzymes, but also gain new mechanistic insights into the investigated catalyst.
Lecture notesThe necessary documents and protocols will be distributed to the participants during the course.
LiteratureGeneral literature to "Directed Evolution" and chorismate mutases, e.g.:

– Taylor, S. V., P. Kast & D. Hilvert. 2001. Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. 40: 3310-3335.

– Jäckel, C., P. Kast & D. Hilvert. 2008. Protein design by directed evolution. Annu. Rev. Biophys. 37: 153-173.

– Roderer, K. & P. Kast. 2009. Evolutionary cycles for pericyclic reactions – Or why we keep mutating mutases. Chimia 63: 313-317.

Further literature will be indicated in the distributed script.
Prerequisites / Notice- This laboratory course will involve experiments that require a tight schedule and (sometimes) long (!) working days.
- The projects of this course are tightly linked to the ones of the Biology BSc course "529-0739-01 Biological Chemistry B: New Enzymes from Directed Evolution Experiments", which takes place as a block course during the month of November. There will be joint lectures for the participants of both courses during that time. The teaching language is English.
- The number of participants for the laboratory class is limited. It is mandatory to sign up for the course directly with P. Kast no later than September 1, prior to the start of the fall semester. Until then it will be decided whether the course will take place.
- A valid registration is considered a commitment for attendance of the entire semester course, as involved material orders and experimental preparations are necessary and, once the class has started, the flow of the experiments must not be interrupted by individual absences. In case of an emergency, please immediately notify P. Kast.
- For more information, see also Link or contact P. Kast directly (HCI F 333, Tel. 044 632 29 08, Link).

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits13 credits
ExaminersP. A. Kast
Typeungraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.
Additional information on mode of examinationCredit points can only be earned if the candidate (i) takes active part in the entire program, (ii) gives an oral presentation on achieved results, and (iii) turns in a final report that is accepted.
Furthermore, each participant's performance will be assessed with regard to (1) effort/participation/presentation, (2) skills or acquisition of skills, and (3) quality of the report to determine whether the class is passed. Additional information will be provided at the beginning of the course.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

General : Special students and auditors need a special permission from the lecturers
Permission from lecturers required for all students

Offered in

ProgrammeSectionType
Chemistry MasterIndustry Internship or Laboratory CourseWInformation