535-0810-00L Gene Technology
| Semester | Autumn Semester 2018 |
| Lecturers | D. Neri |
| Periodicity | yearly recurring course |
| Language of instruction | English |
Courses
| Number | Title | Hours | Lecturers | ||||
|---|---|---|---|---|---|---|---|
| 535-0810-00 G | Gene Technology | 2 hrs |
| D. Neri |
Catalogue data
| Abstract | The course will provide a solid overview of the science and issues in gene technology and its pharmaceutical applications. |
| Learning objective | The aim of the lecture course is to provide a solid overview of gene technology, with a special focus on drug development. Topics: Antibody phage technology, DNA-encoded chemistry, protein modification technology, genome sequencing, transcriptomics, proteomics, functional genomics, principle of drug discovery. The course is suited for advanced undergraduate and early graduate students in pharmaceutical sciences or related fields. |
| Content | 1. Antibody phage technology The antibody molecule V genes, CDRs, basics of antibody engineering Principles of phage display Phagemid and phage vectors Antibody libraries Phage display selection methodologies Other phage libraries (peptides, globular proteins, enzymes) Alternative screening/selection methodologies DNA-encoded chemical libraries 2. Proteins: chemical modification and detection of biomolecular interactions Homo- and hetero-dimerization of proteins Chemical modifications of proteins Antibody-drug conjugates Radioactive labeling of proteins Kinetic association and dissociation constants Affinity constant: definition and its experimental measurement 3. Genomics: Applications to Human Biology Protein cloning and expression DNA sequencing Some foundations of genetic analysis Knock-out technologies Transcriptomics Proteomics Recombinant vaccines 4: Pharmaceuticals: Focus on Discovery Ligand Discovery Half-life extension Cancer therapy Gene therapy |
| Lecture notes | Skript "Gene Technology" by Prof. Dario Neri and slides of the lecture |
Performance assessment
| Performance assessment information (valid until the course unit is held again) | |
Performance assessment as a semester course | |
| ECTS credits | 2 credits |
| Examiners | D. Neri |
| Type | session examination |
| Language of examination | English |
| Repetition | The performance assessment is offered every session. Repetition possible without re-enrolling for the course unit. |
| Mode of examination | written 60 minutes |
| Additional information on mode of examination | Five questions; in addition to English, answers can be given in German, Italian or French |
| Written aids | Keine |
| This information can be updated until the beginning of the semester; information on the examination timetable is binding. | |
Learning materials
| Main link | Skript "Gene Technology" by Prof. Dario Neri |
| Recording | Skript "Gene Technology" by Prof. Dario Neri |
| Learning environment | Skript "Gene Technology" by Prof. Dario Neri |
| Documents | Skript "Gene Technology" by Prof. Dario Neri |
| Additional links | Skript "Gene Technology" by Prof. Dario Neri |
| Only public learning materials are listed. | |
Groups
| No information on groups available. |
Restrictions
| There are no additional restrictions for the registration. |
