636-0205-00L  Lab Course: Mammalian Gene Circuits

SemesterSpring Semester 2018
LecturersY. Benenson
Periodicityyearly recurring course
Language of instructionEnglish
CommentOnly for Biotechnology MSc, Programme Regulations 2017.



Courses

NumberTitleHoursLecturers
636-0205-00 PLab Course: Mammalian Gene Circuits Special students and auditors need a special permission from the lecturers.
The Lab Course will take place Monday/Tuesday 9-17h, 8 days in total, start of this lab course is on Monday, March 26, 2018, end of lab course is on Tuesday, April 24 (no lab course on April 2/3; Easter Holidays)
64s hrsY. Benenson

Catalogue data

AbstractThe students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level.
ObjectiveThe objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization.
ContentThe course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities

Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1.
Week 2: Cloning of the constructs, purification and characterization of DNA constructs
Week 3: Cell culture transfection, microscopy and flow cytometry characterization
Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments.
Lecture notesPreparatory materials will be provided before the start of the course.
LiteratureWill be provided before the course

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits2 credits
ExaminersY. Benenson
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.
Additional information on mode of examinationWritten lab report required

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

General : Special students and auditors need a special permission from the lecturers

Offered in

ProgrammeSectionType
Biotechnology MasterPractical TrainingOInformation