Only for Biotechnology MSc, Programme Regulations 2017.
Courses
Number
Title
Hours
Lecturers
636-0205-00 P
Lab Course: Mammalian Gene Circuits
The Lab Course will take place Monday/Tuesday 9-17h, 8 days in total, start of this lab course is on Monday, March 26, 2018, end of lab course is on Tuesday, April 24 (no lab course on April 2/3; Easter Holidays)
The students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level.
Learning objective
The objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization.
Content
The course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities
Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1. Week 2: Cloning of the constructs, purification and characterization of DNA constructs Week 3: Cell culture transfection, microscopy and flow cytometry characterization Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments.
Lecture notes
Preparatory materials will be provided before the start of the course.
Literature
Will be provided before the course
Performance assessment
Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
: Special students and auditors need a special permission from the lecturers