551-1403-00L  Imaging Bacterial Cells in a Native State by Electron Cryotomography

SemesterAutumn Semester 2017
LecturersM. Pilhofer
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 3.

The enrolment is done by the D-BIOL study administration.


AbstractThe goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures
ObjectiveStudents will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization.
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