X-Ray Crystallographic Structure Determination and Biophysics
Permission from lecturers required for all students.
Block course will be held two times, once in the 3rd- and once in the 4th quarter of the spring semester The practical part of the course for 6 out of 8 students will be done at the Laboratory of Biolmolecular Research (BMR), Paul Scherrer Institut (http://www.psi.ch/lbr). For location please see also under the link http://www.psi.ch/how-to-find-us Number of participants limited to 8 per quarter
This course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy.
Learning objective
The course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques.
Content
Micro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map.
Prerequisites / Notice
The students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation.
Performance assessment
Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course