551-0352-00L Introduction to Mass Spectrometry-Based Proteomics
| Semester | Autumn Semester 2022 |
| Lecturers | L. Gillet, P. Picotti |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. |
Courses
| Number | Title | Hours | Lecturers | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 551-0352-00 P | Introduction to Mass Spectrometry-Based Proteomics Permission from lecturers required for all students.
Block course in the 1st quarter of the autumn semester | 100s hrs |
| L. Gillet, P. Picotti |
Catalogue data
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. |
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins |
Performance assessment
| Performance assessment information (valid until the course unit is held again) | |
Performance assessment as a semester course | |
| ECTS credits | 6 credits |
| Examiners | L. Gillet, P. Picotti |
| Type | graded semester performance |
| Language of examination | English |
| Repetition | Repetition only possible after re-enrolling for the course unit. |
| Additional information on mode of examination | Students are obliged to be present throughout the block course. Cancellation: If you have to deregister from a course that has been assigned to you (just emergency reasons), please notify in written the course coordinator at least four weeks before the course starts, for courses in the 1st quarter, a cancellation period of one week applies. The study secretariat D-BIOL must also be informed (email CC to studies@biol.ethz.ch) so that the enrollment is deleted. Otherwise the course is considered as "failed". |
Learning materials
| Moodle course | Moodle-Kurs / Moodle course |
| Only public learning materials are listed. | |
Groups
| No information on groups available. |
Restrictions
| General | Permission from lecturers required for all students |
Offered in
| Programme | Section | Type | |
|---|---|---|---|
| Biology Bachelor | Block Courses in 1st Quarter of the Semester | W |  |
| Biology Bachelor | Block Courses in 1st Quarter of the Semester | W | |