701-1425-01L Genetic Diversity: Techniques

Semester	Autumn Semester 2020
Lecturers	A. M. Minder Pfyl
Periodicity	yearly recurring course
Language of instruction	English
Comment	Number of participants limited to 8. Selection of the students: order of registration Registration until 18.10.2020. General safety regulations for all this course: -Whenever possible the distance rules have to be respected -All students have to wear masks throughout the course (keep reserve masks ready) -The installation and activation of the Swiss Covid-App is highly encouraged -Any additional rules for individual courses have to be respected -Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible

Abstract	This course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction protocols, quality control measurements, SNP genotyping and gene expression techniques will be addressed.
Learning objective	To learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing etc. A course for practicioners.
Content	After an introduction (one afternoon), students will have 3 weeks to work independently in groups of two through different protocols. At the end the whole class meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques. Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR.
Lecture notes	Material will be handed out in the course.
Literature	Material will be handed out in the course.
Prerequisites / Notice	Two afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student.