Only for Biotechnology MSc, Programme Regulations 2017. Attention: This lab course was offered in previous semesters with the number: 626-0806-00L "Laboratory Course Stem Cell Purification, Culture and Manipulation”. Students that already passed course 626-0806-00L cannot receive credits for course 636-0207-00L.
Courses
Number
Title
Hours
Lecturers
636-0207-00 P
Lab Course: Cellular Engineering Stem Cells
This lab course will take place after the spring semester: Monday, June 18 - Friday, June 29, 10 full days
Mammalian stem cells of different organs are purified, cultured, differentiated, analyzed and manipulated. Plasmids and viral vectors will be cloned, produced and transfected / transduced to manipulate stem cells. Computational and analytical molecular biology methods, FACS and imaging and lectures complement the program.
Learning objective
Independent planning and conducting of experiments with mammalian stem cells including all steps from culturing different cell lines to DNA transfection / transduction and expression analysis by different analytical methods. Documenting and writing a report on conducted experiments and results.
Content
Practical course on purification of primary mammalian stem cells, culture of primary stem cells and stem cell lines, characterization, manipulation and differentiation of stem cells. Construction of plasmids or viral vectors for gene expression, DNA transfer by transfection and transduction, analysis of gene expression by fluorescent proteins, PCR, fluorescence-activated cell sorting (FACS), imaging. Documentation of experiments in a laboratory journal, writing of a report on the experiments and results.
Performance assessment
Performance assessment information (valid until the course unit is held again)
Repetition only possible after re-enrolling for the course unit.
Additional information on mode of examination
Group examination (colloquium) of 3 students at the end of the course, 20 Minutes per group. Written report on experiments and results will be graded as pass/fail and is required to pass the course.
Learning materials
No public learning materials available.
Only public learning materials are listed.
Groups
No information on groups available.
Restrictions
General
: Special students and auditors need a special permission from the lecturers
Performance assessment as a semester course
: Special students and auditors need a special permission from the lecturers