551-0352-00L Protein Analysis by Mass Spectrometry
| Semester | Spring Semester 2018 |
| Lecturers | L. Gillet, B. Collins, P. Picotti |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. |
Courses
| Number | Title | Hours | Lecturers | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 551-0352-00 G | Protein Analysis by Mass Spectrometry Permission from lecturers required for all students.
Block course in the 2nd quarter of the spring semester (combined with block course 551-0362-00 in the 3rd quarter of the spring semester) | 100s hrs |
| L. Gillet, B. Collins, P. Picotti |
Catalogue data
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. |
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins |
Performance assessment
| Performance assessment information (valid until the course unit is held again) | |
Performance assessment as a semester course | |
| ECTS credits | 6 credits |
| Examiners | B. Collins, L. Gillet, P. Picotti |
| Type | graded semester performance |
| Language of examination | English |
| Repetition | Repetition only possible after re-enrolling for the course unit. |
Learning materials
| No public learning materials available. | |
| Only public learning materials are listed. |
Groups
| No information on groups available. |
Restrictions
| General | Permission from lecturers required for all students |
Offered in
| Programme | Section | Type | |
|---|---|---|---|
| Biology Bachelor | Block Courses in 2nd Quarter of the Semester | W |  |