551-1412-00L Molecular and Structural Biology IV: Visualizing Macromolecules by X-Ray Crystallography and EM
| Semester | Frühjahrssemester 2019 |
| Dozierende | N. Ban, D. Böhringer, T. Ishikawa, M. A. Leibundgut, K. Locher, M. Pilhofer, K. Wüthrich, weitere Dozierende |
| Periodizität | jährlich wiederkehrende Veranstaltung |
| Lehrsprache | Englisch |
Lehrveranstaltungen
| Nummer | Titel | Umfang | Dozierende | ||||
|---|---|---|---|---|---|---|---|
| 551-1412-00 V | Molecular and Structural Biology IV: Visualizing Macromolecules by X-Ray Crystallography and EM | 2 Std. |
| N. Ban, D. Böhringer, T. Ishikawa, M. A. Leibundgut, K. Locher, M. Pilhofer, K. Wüthrich, weitere Dozierende |
Katalogdaten
| Kurzbeschreibung | This course provides an in-depth discussion of two main methods to determine the 3D structures of macromolecules and complexes at high resolution: X-ray crystallography and cryo-electron microscopy. Both techniques result in electron density maps that are interpreted by atomic models. |
| Lernziel | Students will obtain the theoretical background to understand structure determination techniques employed in X-ray crystallography and electron microscopy, including diffraction theory, crystal growth and analysis, reciprocal space calculations, interpretation of electron density, structure building and refinement as well as validation. The course will also provide an introduction into the use of cryo-electron tomography to visualize complex cellular substructures at sub-nanometer resolutions, effectively bridging the resolution gap between optical microscopy and single particle cryo-electron microscopy. Lectures will be complemented with practical sessions where students will have a chance to gain hands on experience with sample preparation, data processing and structure building and refinement. |
| Inhalt | February 22 Lecture 1 Prof. Dr. Kurt Wüthrich History of Structural Molecular Biology March 1 Lecture 2 Prof. Dr. Kaspar Locher X-ray diffraction from macromolecular crystals March 8 Lecture 3 Prof. Dr. Kaspar Locher Data collection and statistics, phasing methods March 15 Lecture 4 Prof. Dr. Nenad Ban Crystal symmetry and space groups March 22 Lecture 5 Ban Lab Practical session with X-ray data processing March 29 Lecture 6 Prof. Dr. Takashi Ishikawa Principle of cryo-EM for biological macromolecules I, including hardware of TEM and detectors, image formation principle (phase contrast, spherical aberration, CTF), 3D reconstruction (central-section theorem, backprojection, missing information) April 5 Lecture 7 Dr. Daniel Boehringer Single particle analysis, including principle (projection matching, random conical tilt, angular reconstitution) April 12 Lecture 8 Ban Lab Practical session including specimen preparation (cryo, negative stain, visit to ScopeM May 3 Lecture 9 Prof. Dr. M. Pilhofer Tomography I, including basics and subtomogram averaging May 10 Lecture 10 Ban Lab Practical session with example initial EM data processing May 17 Lecture 11 Prof. Dr. Martin Pilhofer Practical session (including recent techniques, including cryo-FIB) May 24 Lecture 12 Prof. Dr. Nenad Ban EM and X-ray structure building, refinement, validation and interpretation May 31 Lecture 13 Ban Lab Practical session with model building and refinemen |
Leistungskontrolle
| Information zur Leistungskontrolle (gültig bis die Lerneinheit neu gelesen wird) | |
Leistungskontrolle als Semesterkurs | |
| ECTS Kreditpunkte | 4 KP |
| Prüfende | N. Ban, D. Böhringer, T. Ishikawa, M. A. Leibundgut, K. Locher, M. Pilhofer, K. Wüthrich |
| Form | Sessionsprüfung |
| Prüfungssprache | Englisch |
| Repetition | Die Leistungskontrolle wird in jeder Session angeboten. Die Repetition ist ohne erneute Belegung der Lerneinheit möglich. |
| Prüfungsmodus | schriftlich 60 Minuten |
| Hilfsmittel schriftlich | Keine |
| Diese Angaben können noch zu Semesterbeginn aktualisiert werden; verbindlich sind die Angaben auf dem Prüfungsplan. | |
Lernmaterialien
| Keine öffentlichen Lernmaterialien verfügbar. | |
| Es werden nur die öffentlichen Lernmaterialien aufgeführt. |
Gruppen
| Keine Informationen zu Gruppen vorhanden. |
Einschränkungen
| Keine zusätzlichen Belegungseinschränkungen vorhanden. |
Angeboten in
| Studiengang | Bereich | Typ | |
|---|---|---|---|
| Biologie Master | Wahlpflicht Masterkurse | W |  |
| Biologie Master | Wahlpflicht Masterkurse | W | |
| Biologie Master | Wahlpflicht Masterkurse | W | |
| Biologie Master | Wahlpflicht Masterkurse | W | |