636-0113-00L  Genome Engineering

SemesterFrühjahrssemester 2018
DozierendeR. Platt
Periodizitätjährlich wiederkehrende Veranstaltung

KurzbeschreibungThis course is an introduction to genome engineering and an examination of recent advancements and future challenges. It covers the discovery and development of gene editing technologies and their applications in basic and applied research. The focus is on gaining an in-depth molecular and cellular understanding of the technologies and also insight into how these tools can be used and further deve
LernzielThe objective of this course is to learn how gene editing technologies function at the molecular and cellular level, and how they are leveraged to understand the role of genetic elements in biological processes. Students will be introduced to the history and motivation behind the discovery and development of transformative genome engineering technologies, and also gain insight into the ethical, safety, and regulatory facets shaping the field.
InhaltThe course content is comprised of lectures, discussions of important literature in the field, and a project. Lectures in Genome Engineering will be technology-focused and incorporate: 1) historical context to motivate the need for developing the technology, 2) development of the technology from concept to robust tool, 3) methods to discover, characterize, and evaluate the technology, and 4) applications of the technology in basic and applied research. Discussions of important literature in the field will be conducted in class, one course meeting following a lecture covering the topic material and assignment of the reading. The project will be team-based and entail devising a solution to a critical need in the field.

Main topics:
--Discovery and development of genome editing technologies
--The prokaryotic adaptive immune system CRISPR-Cas
--Genome engineering methods for generating genetically engineered model systems
--Genotype-phenotype linkage via genetic screens
--Massively paralleled perturbation and phenotyping
--Gene editing tools as genetic recording devices
--Gene editing tools as diagnostics and therapeutics
--Ethics, safety, and regulatory facets of genome engineering
SkriptHandout during the course.
LiteraturPeer-reviewed scientific publications will be assigned to complement lecture content.