Benoît Pugin: Catalogue data in Spring Semester 2019

Name Dr. Benoît Pugin
Professur f. Lebensm.biotechnol.
ETH Zürich, LFV D 43
Schmelzbergstrasse 7
8092 Zürich
DepartmentHealth Sciences and Technology

752-5004-00LFood Biotechnology Laboratory Course Restricted registration - show details
Number of participants limited to 48

Prerequisites: Participation in the courses Food Biotechnology (752-5001-00L) and Fermented Milk Products (752-5002-00L).
3 credits5PA. Greppi, C. Lacroix, B. Pugin
AbstractThe students carry out processes for the production of important fermented foods. Experimental blocks: fermentation in state of the art bioreactors; cheese production in a modern pilot cheese plant and microbial and metabolites analysis during cheese ripening; Joghurt production and protective cultures; impact of functional food tested in an in vitro intestinal digestion model.
ObjectiveTo demonstrate the operation of complete fermentation processes for the production of selected fermented foods and bioingredients; running of small fermenters to understand the effects of important parameters of fermentation processes, including raw materials, and their control; to better understand functional food; to analyze the effects of defined fermentation conditions on the quality of final products; to scientifically present experimental approaches and data.
ContentThis laboratory course includes four experimental blocks:

- Fermentation in state of the art bioreactors: preparation of equipment, media and starter culture, monitoring and control of the productive phase, analysis of samples for biomass and metabolic products, data interpretation and calculation of kinetics, comparison of different fermentation conditions;

- Cheese production in a modern pilot cheese plant and cheese ripening: manufacturing of model cheeses at Agroscope Liebefeld-Posieux, cheese ripening, monitoring of metabolites and microbiological composition and hygienic quality of cheese, estimation of process efficiency and yield calculation, comparison of different fermentation conditions;

- Yoghurt production in small scale with different starter cultures, assaying the principle of biopreservation by using protective cultures against yeast and mould growth and microbial analysis of their efficacy during storage of the fermented products.

- Impact of different food ingredients reported as functional food on the microbiota in a in vitro small scale intestinal model fermentation.
Lecture notesA complete course document will be provided.
LiteratureReferences will be indicated in the lab course manual
Prerequisites / Notice1. Absolvierung der Vorlesung "Food Biotechnology I" (752-5001-00) inklusive Teilnahme an der Semesterendprüfung.

2. Absolvierung der Vorlesung "Fermented Milk Products" (752-5002-00) parallel zum Kursbesuch.