Bernard Collins: Catalogue data in Spring Semester 2018 |
| Name | Dr. Bernard Collins |
| Department | Biology |
| Relationship | Lecturer |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0352-00L | Protein Analysis by Mass Spectrometry  Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. | 6 credits | 7G | L. Gillet, B. Collins, P. Picotti | |
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. | ||||
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins | ||||