Ludovic Gillet: Catalogue data in Autumn Semester 2022 |
| Name | Dr. Ludovic Gillet |
| Address | Inst. f. Molekulare Systembiologie ETH Zürich, HPM F 42 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 31 91 |
| Fax | +41 44 633 10 51 |
| gillet@imsb.biol.ethz.ch | |
| Department | Biology |
| Relationship | Lecturer |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0352-00L | Introduction to Mass Spectrometry-Based Proteomics  Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | L. Gillet, P. Picotti | |
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. | ||||
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins | ||||