Donald Hilvert: Catalogue data in Autumn Semester 2020

Name	Prof. em. Dr. Donald Hilvert
Field	Organische Chemie
Address	Dep. Chemie und Angew. Biowiss. ETH Zürich, HCI H 327 Vladimir-Prelog-Weg 1-5/10 8093 Zürich SWITZERLAND
Telephone	+41 44 632 31 76
E-mail	donald.hilvert@org.chem.ethz.ch
Department	Chemistry and Applied Biosciences
Relationship	Professor emeritus

Number	Title	ECTS	Hours	Lecturers
529-0290-00L	Organic Chemistry (Seminar)	0 credits	2S	E. M. Carreira, J. W. Bode, D. Hilvert, H. Wennemers, R. Zenobi
Abstract	Seminars on Current Topics in Organic Chemistry, Chemical Biology, and Analytical Chemistry.
Learning objective	Awareness of contemporary trends in science.
529-0299-00L	Organic Chemistry	0 credits	1.5K	J. W. Bode, E. M. Carreira, P. Chen, D. Hilvert, H. Wennemers, R. Zenobi
Abstract	Updates on Research and Contemporary Literature in Organic Chemistry and Chemical Biology.
Learning objective	Problem solving in organic chemistry and chemical biology.
529-0731-00L	Nucleic Acids and Carbohydrates Note for BSc Biology students: Only one of the two concept courses 529-0731-00 Nucleic Acids and Carbohydrates (autumn semester) or 529-0732-00 Proteins and Lipids (spring semester) can be counted for the Bachelor's degree.	6 credits	3G	D. Hilvert, P. A. Kast, S. J. Sturla, H. Wennemers
Abstract	Structure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
Learning objective	Structure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
Content	Structure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
Lecture notes	No script; illustrations from the original literature relevant to the individual lectures will be provided weekly (typically as handouts downloadable from the Moodle server).
Literature	Mainly based on original literature, a detailed list will be distributed during the lecture
529-0733-01L	Enzymes	6 credits	3G	D. Hilvert
Abstract	Principles of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme-catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
Learning objective	Overview of enzymes, enzyme-catalyzed reactions and metabolic processes.
Content	Principles of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
Lecture notes	A script will not be handed out.
Literature	General: T. Bugg, An Introduction to Enzyme and Coenzyme Chemistry, Blackwell Science Ltd., Oxford, 1997. In addition, citations from the original literature relevant to the individual lectures will be assigned weekly.
529-0739-10L	Biological Chemistry A: Technologies for Directed Evolution of Enzymes Advanced laboratory course or internship depending on lab course Biological Chemistry B Candidates must inquire with P. Kast no later than September 1st whether course will take place (no self-enrollment) Further information to registration and work hours: www.kast.ethz.ch/teaching.html	13 credits	16P	P. A. Kast, D. Hilvert
Abstract	During this semester course, methodologies will be taught for biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains.
Learning objective	All technologies used for the experiments will be explained to the students in practice with the goal that they will be able to independently apply them for the course project and in future research endeavors. After the course, an individual report about the results obtained has to be prepared.
Content	This class conducts and supports experiments for a specifically designed genuine research project. We will carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. The relevant technologies will be taught to the students, such as the preparation of competent cells, production and isolation of DNA fragments, transformation of gene libraries, and DNA sequencing. The course participants will generate a variety of different variants of a chorismate mutase. Individual enzyme catalysts will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters, their molecular mass, and the integrity of the protein structure. The students will present the results obtained from their individual evolution experiments at the end of the semester. We expect that during this lab course we will not only generate novel enzymes, but also gain new mechanistic insights into the investigated catalyst.
Lecture notes	The necessary documents and protocols will be distributed to the participants during the course.
Literature	General literature to "Directed Evolution" and chorismate mutases, e.g.: – Taylor, S. V., P. Kast & D. Hilvert. 2001. Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. 40: 3310-3335. – Jäckel, C., P. Kast & D. Hilvert. 2008. Protein design by directed evolution. Annu. Rev. Biophys. 37: 153-173. – Roderer, K. & P. Kast. 2009. Evolutionary cycles for pericyclic reactions – Or why we keep mutating mutases. Chimia 63: 313-317. Further literature will be indicated in the distributed script.
Prerequisites / Notice	- This laboratory course will involve experiments that require a tight schedule and (sometimes) long (!) working days. - The projects of this course are tightly linked to the ones of the Biology BSc course "529-0739-01 Biological Chemistry B: New Enzymes from Directed Evolution Experiments", which takes place as a block course during the month of November. There will be joint lectures for the participants of both courses during that time. The teaching language is English. - The number of participants for the laboratory class is limited. It is mandatory to sign up for the course directly with P. Kast no later than September 1, prior to the start of the fall semester. Until then it will be decided whether the course will take place. - A valid registration is considered a commitment for attendance of the entire semester course, as involved material orders and experimental preparations are necessary and, once the class has started, the flow of the experiments must not be interrupted by individual absences. In case of an emergency, please immediately notify P. Kast. - For more information, see also http://www.kast.ethz.ch/teaching.html or contact P. Kast directly (HCI F 333, Tel. 044 632 29 08, kast@org.chem.ethz.ch).