Rudolf Glockshuber: Catalogue data in Spring Semester 2022 |
| Name | Prof. em. Dr. Rudolf Glockshuber |
| Field | Molecular Biology |
| Address | Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK F 7 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 68 19 |
| rudi@mol.biol.ethz.ch | |
| Department | Biology |
| Relationship | Professor emeritus |
| Number | Title | ECTS | Hours | Lecturers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 529-0074-00L | BCB IV: Analytical Biochemistry and Biophysics Only for students of Biochemistry - Chemical Biology | 5 credits | 7P | B. Rubi, J. W. Bode, R. Glockshuber, E. C. Meister | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | Practical Introduction to Experimental Methods in Analytical and Bioanalytical Chemistry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | To acquire a sound knowledge of the instrumentation and methods for the analysis of molecules, including organic compounds and biological molecules. To learnabout the most important practical techniques in analytical chemistry. To write critical reports for each experiment and analytical technique. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | The course will comprise of maximum 10 experiments from the following themes: Spectroscopic structural characterisation, analytical characterisation, Bio-physical characterisation. Experiments that will be part of this curriculum are: Circular dichroism (CD) spectroscopy, UV spectroscopy, Mass Spectromety, Nuclear Magnetic Resonance, HPLC and Size exclusion separations, Isothermal titration calorimetry as well as a number of other bio-physical and biological characterisation techniques. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lecture notes | Learning Materials will be made available on an online platform (web page or Moodle) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | Learning Materials will be made available on an online platform (web page or Moodle) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Prerequisites / Notice 529-0011-04 "Allgemeine Chemie (Praktikum)" 529-0051-00 "Analytische Chemie" I (3. Semester) 529-0058-00 "Analytische Chemie II" (4. Semester) enrolment in parallel to the lab class, or completed in an earlier semester. 551-0128-00L Fundamentals of Biology I BCB Students should also be enrolled in "Instumentalanalyse organischer Verbindungen". | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Competencies |
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| 551-0013-AAL | Biochemistry Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement. Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit. | 2 credits | 4R | R. Glockshuber | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | The lecture is a basic introductory course on the molecular principles of biology for students who need to pass this course for admission to their MSc curriculum. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | The goal of this course is to give the students a basic understanding of the molecules that build a cell and make it function, and the basic principles of metabolism and molecular genetics | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | The course content is based on the following chapters of the textbook Biochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York) Chapter 1: The molecular design of life Chapter 2: Protein composition and structure Chapter 3: Exploring proteins and proteomes Chapter 4: DNA, RNA and the flow of information Chapter 5: Exploring Genes and Genomes Chapter 7: Hemoglobin Chapter 8: Enzymes and the basic concepts of catalysis Chapter 11: Carbohydrates Chapter 12: Lipids and cell membranes Chapter 15: Metabolism: Basic concepts and design | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | Biochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-0438-00L | Protein Folding, Assembly and Degradation  Number of participants limited to 20. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | R. Glockshuber, E. Weber-Ban | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | Students will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | The course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | The students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work. Participation in one of the following projects will be possible: Projects of the Glockshuber group: - Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria. - Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits. - Identification of intermediates in the aggregation of the human Abeta peptide Experimental work on these projects involves - Molecular cloning, recombinant protein production in E. coli and protein purification - Protein crystallization - Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy - Analysis of rapid reactions by stopped-flow fluorescence - Negative-stain electron microscopy - Light scattering Projects of the Weber-Ban group: - Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Attendance of the concept course "Biomolecular Structure and Mechanism I" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-1324-00L | Biochemistry | 5 credits | 4G | K. Locher, N. Ban, R. Glockshuber, J. Piel, E. Weber-Ban | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | The lecture teaches central reactions and metabolic pathways of biochemistry as well as molecular biology with emphasis on chemical and partly biophysical aspects. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | Understanding of the most important metabolic processes involved in cellular energy production and storage as well as selected biosynthetic processes (incl. sugars, fats, steroids etc). Understanding of the molecular processes involved in replication, transcription and translation. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Some lecture units are held in English. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-1402-00L | Molecular and Structural Biology VI: Biophysical Analysis of Macromolecular Mechanisms This course is strongly recommended for the Masters Major "Biology and Biophysics". | 4 credits | 2V | R. Glockshuber, T. Ishikawa, S. Jonas, B. Schuler, E. Weber-Ban | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | The course is focussed on biophysical methods for characterising conformational transitions and reaction mechanisms of proteins and biological mecromolecules, with focus on methods that have not been covered in the Biology Bachelor Curriculum. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | The goal of the course is to give the students a broad overview on biopyhsical techniques available for studying conformational transitions and complex reaction mechanisms of biological macromolecules. The course is particularly suited for students enrolled in the Majors "Structural Biology and Biophysics", "Biochemistry" and "Chemical Biology" of the Biology MSc curriculum, as well as for MSc students of Chemistry and Interdisciplinary Natural Sciences". | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | The biophysical methods covered in the course include advanced reaction kinetics, methods for the thermodynamic and kinetic analysis of protein-ligand interactions, static and dynamic light scattering, analytical ultracentrifugation, spectroscopic techniques such as fluorescence anisotropy, fluorescence resonance energy transfer (FRET) and single molecule fluorescence spectrosopy, modern electron microscopy techniques, atomic force microscopy, and isothermal and differential scanning calorimetry. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lecture notes | Course material from the individual lecturers wil be made available at the sharepoint website https://team.biol.ethz.ch/e-learn/551-1402-00L | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Finished BSc curriculum in Biology, Chemistry or Interdisciplinary Natural Sciences. The course is also adequate for doctoral students with research projects in structural biology, biophysics, biochemistry and chemical biology. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-1620-00L | Molecular Biology, Biophysics | 1 credit | 1K | R. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, K. Wüthrich | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | The course consists of a series of research seminars on Structural Biology and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | The goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Information on the individual seminars is provided on the following websites: http://www.structuralbiology.unizh.ch/events005.asp http://www.biol.ethz.ch/dbiol-cal/index | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||