Alvar Diego Gossert: Katalogdaten im Frühjahrssemester 2019

Name	Herr Dr. Alvar Diego Gossert
Adresse	Dep. Biologie ETH Zürich, HPP L 25.2 Hönggerbergring 64 8093 Zürich SWITZERLAND
Telefon	+41 44 633 34 55
E-Mail	alvar.gossert@biol.ethz.ch
URL	https://bnsp.ethz.ch/people/gossert.html
Departement	Biologie
Beziehung	Dozent

Nummer	Titel	ECTS	Umfang	Dozierende
551-0434-00L	NMR Spectroscopy in Biology Number of participants limited to 6. The enrolment is done by the D-BIOL study administration.	6 KP	7G	F. Allain, A. D. Gossert, K. Wüthrich
Kurzbeschreibung	In this block course, students actively participate in ongoing research projects in the research groups of Profs. Allain, Wüthrich and Dr. Gossert. The students will be tutored in their experimental work by experienced postdoc students. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as exercises and literature work.
Lernziel	The course provides first "hands on" insight into applications of NMR spectroscopy in biological sciences. The course should enable the students to understand the potential and limitations of NMR applied to biological problems.
Inhalt	The topics include studies of proteins, RNA and protein-RNA interactions, Participation in one of the following projects will be possible: - NMR of RNA - NMR of several protein-RNA complexes (hnRNPF, nPTB, SR proteins) - NMR studies of protein-ligand interactions - dynamics of protein-RNA complexes - Segmental isotopic labeling to study multidomain proteins - NMR Methods Development
Skript	No script
Literatur	Lists of individual reading assignments will be handed out.
551-1414-00L	Molecular and Structural Biology V: Studying Macromolecules by NMR and EPR	4 KP	2V	F. Allain, A. D. Gossert, G. Jeschke, K. Wüthrich
Kurzbeschreibung	The course provides an overview of experimental methods for studying function and structure of macromolecules at atomic resolution in solution. The two main methods used are Nuclear Magnetic Resonance (NMR) spectroscopy and Electron Paramagnetic Resonance (EPR) spectroscopy.
Lernziel	Insight into the methodology, areas of application and limitations of these two methods for studying biological macromolecules. Practical exercises with spectra to have hands on understanding of the methodology.
Inhalt	Part I: Historical overview of structural biology. Part II: Basic concepts of NMR and initial examples of applications. 2D NMR and isotope labeling for studying protein function and molecular interactions at atomic level. Studies of dynamic processes of proteins in solution. Approaches to study large particles. Methods for determination of protein structures in solution. Part III: NMR methods for structurally characterizing RNA and protein-RNA complexes. Part IV: EPR of biomolecules
Literatur	1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience. 2) Dominguez et al, Prog Nucl Magn Reson Spectrosc. 2011 Feb;58(1-2):1-61. 3) Duss O et al, Methods Enzymol. 2015;558:279-331.
551-1616-00L	Methods for Studies of Biological Macromolecules by NMR	1 KP	2S	A. D. Gossert
Kurzbeschreibung	In this course topics relevant to structure determination of biological macromolecules by solution state NMR spectroscopy are discussed. The course is tailored to advanced students, mainly PhD students and postdocs in structural biology who have experience with applications of NMR spectroscopy. The individual participants present various topics in form of a seminar.
Lernziel	The students will actively participate in the course which is held in the form of a seminar. Individual students will prepare particular topics of the course based on literature references and present the material in form of a seminar to their fellow students. In short, the students learn to actively participate in discussions and to prepare a presentation of a scientific topic which was mostly unknown to them before.