Kaspar Locher: Katalogdaten im Frühjahrssemester 2015 |
| Name | Herr Prof. Dr. Kaspar Locher |
| Lehrgebiet | Molekulare Membranbiologie |
| Adresse | Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK G 11 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND |
| Telefon | +41 44 633 39 91 |
| locher@mol.biol.ethz.ch | |
| URL | http://www.locherlab.ethz.ch |
| Departement | Biologie |
| Beziehung | Ordentlicher Professor |
| Nummer | Titel | ECTS | Umfang | Dozierende | |
|---|---|---|---|---|---|
| 551-0142-00L | Structure Determination of Biological Macromolecules by X-ray Crystallography and NMR | 6 KP | 3G | F. Allain, N. Ban, K. Locher, G. Wider, K. Wüthrich, weitere Dozierende | |
| Kurzbeschreibung | Die Vorlesung gibt einen Überblick über experimentelle Methoden zur Strukturaufklärung von Makromolekülen mit atomarer Auflösung. | ||||
| Lernziel | Einsicht in die Methodik, Applikationsgebiete und Einschränkungen von zwei Hauptmethoden für die Strukturbestimmung von biologischen Makromolekülen. | ||||
| Inhalt | Teil I: Methodik zur Ermittlung der Struktur von Proteinen und makromolekularen Komplexen mittels Röntgendiffraktion von Einkristallen. Teil II: Methodik der Ermittlung von Proteinstrukturen in Lösung mittels kernmagnetischer Resonanzspektroskopie (NMR). Experimentelle Ansätze zur Charakterisierung der intramolekularen Dynamik von Proteinen. | ||||
| Literatur | 1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience. 2) Blow, D. Outline of Crystallography for Biologists. Oxford University Press. | ||||
| 551-1323-AAL | Fundamentals of Biology II: Biochemistry and Molecular Biology  Die Lerneinheit kann nur von MSc Studierenden mit Zulassungsauflagen belegt werden. | 4 KP | 11R | R. Glockshuber, N. Ban, K. Locher, E. Weber-Ban | |
| Kurzbeschreibung | Amino acids; structure of proteins; folding; dynamics and evolution; protein purification; sugars and polysaccharides; lipids and membranes. Enzymatic catalysis. Metabolism; Gene expression and propagation of genetic information; structure of DNA; transcription; protein biosynthesis; DNA replication. Gene technology; production of recombinant proteins. | ||||
| Lernziel | Knowledge on the structural construction of biological macromolecules, principles of enyme catalysed reactions, basics of molecular genetics and protein biochemistry, basic mechanisms of metabolism and of DNA replication and gene expression. | ||||
| Inhalt | Part 1: Biomolecules; amino acids; covalent assembly of proteins; three dimensional structure of proteins; folding; dynamics and evolution of proteins; methods of protein purification; sugars and polysaccharides; lipids and membranes. Part 2: Enzymatic catalysis: classes of enzymes; kinetics of non catalysed versus catalysed reactions. Examples for the mechanisms of enzyme catalysis. Part 3: Metabolism: Principles of metabolic pathways in living cells; glycolysis; glycogen metabolism; mechanisms of membrane transport; citric acid cycle; electron transport and oxidative phosphorylation. Part 4: Gene expression and propagation of genetic information; structure of DNA; DNA modifying enzymes and manupulation of nucleic acids; transcription; protein biosynthesis; DNA replication. Part 5: Gene technology; production of recombinant proteins | ||||
| Literatur | "Biochemistry" (Voet & Voet; Wiley & Sons, 2nd edition). | ||||
| 551-1556-00L | X-Ray Crystallographic Structure Determination and Biophysics  Number of participants limited to 8 per quarter | 6 KP | 7G | K. Locher, G. Schertler, D. Veprintsev | |
| Kurzbeschreibung | This course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy. | ||||
| Lernziel | The course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques. | ||||
| Inhalt | Micro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map. | ||||
| Voraussetzungen / Besonderes | The students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation. | ||||
| 551-1620-00L | Molecular Biology, Biophysics | 1 KP | 1K | R. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, G. Wider, K. Wüthrich | |
| Kurzbeschreibung | The course consists of a series of research seminars on Structural Biology and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. | ||||
| Lernziel | The goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics. | ||||
| Voraussetzungen / Besonderes | Information on the individual seminars is provided on the following websites: http://www.structuralbiology.unizh.ch/events005.asp http://www.biol.ethz.ch/dbiol-cal/index | ||||