Frédéric Allain: Katalogdaten im Frühjahrssemester 2018 |
| Name | Herr Prof. Dr. Frédéric Allain |
| Lehrgebiet | Biomolekulare NMR |
| Adresse | Institut für Biochemie ETH Zürich, HPP L 14.1 Hönggerbergring 64 8093 Zürich SWITZERLAND |
| Telefon | +41 44 633 39 40 |
| Fax | +41 44 633 12 94 |
| allain@bc.biol.ethz.ch | |
| Departement | Biologie |
| Beziehung | Ordentlicher Professor |
| Nummer | Titel | ECTS | Umfang | Dozierende | |
|---|---|---|---|---|---|
| 551-0307-01L | Molecular and Structural Biology II: From Gene to Protein D-BIOL students are obliged to take part I and part II as a two-semester course. | 3 KP | 2V | N. Ban, F. Allain, M. Pilhofer | |
| Kurzbeschreibung | This course will cover advanced topics in molecular biology and biochemistry with emphasis on the structure and function of cellular assemblies involved in expression and maintenance of genetic information. We will cover the architecture and the function of molecules involved in DNA replication, transcription, translation, nucleic acid packaging in viruses, RNA processing, and CRISPER/CAS system. | ||||
| Lernziel | Students will gain a deep understanding of large cellular assemblies and the structure-function relationships governing their function in fundamental cellular processes ranging from DNA replication, transcription and translation. The lectures throughout the course will be complemented by exercises and discussions of original research examples to provide students with a deeper understanding of the subjects and to encourage active student participation. | ||||
| Inhalt | Advanced class covering the state of the research in structural molecular biology of basic cellular processes with emphasis on the function of large cellular assemblies. | ||||
| Skript | Updated handouts will be provided during the class. | ||||
| Literatur | The lecture will be based on the latest literature. Additional suggested literature: Branden, C., and J. Tooze, Introduction to Protein Structure, 2nd ed. (1995). Garland, New York. | ||||
| 551-0434-00L | NMR Spectroscopy in Biology  Number of participants limited to 6. The enrolment is done by the D-BIOL study administration. | 6 KP | 7G | F. Allain, A. D. Gossert, K. Wüthrich | |
| Kurzbeschreibung | In this block course, students actively participate in ongoing research projects in the research groups of Profs. Allain, Wider and Wüthrich. The students will be tutored in their experimental work by doctoral or postdoctoral students. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises and literature work. | ||||
| Lernziel | The course provides first "hands on" insight into applications of NMR spectroscopy in biological sciences. The course should enable the students to understand the potential and limitations of NMR applied to biological problems. | ||||
| Inhalt | The topics include studies of proteins, RNA and protein-RNA interactions, Participation in one of the following projects will be possible: - NMR of RNA - NMR of several protein-RNA complexes (hnRNPF, nPTB, SR proteins) - NMR studies of glycoproteins - dynamics of protein-RNA complexes - Segmental isotopic labeling to study multidomain proteins -Structural and dynamic properties of FtsZ, the bacterial homolog of tubulin - investigations of the ubiquitinom - NMR Methods Development | ||||
| Skript | No script | ||||
| Literatur | Lists of individual reading assignments will be handed out. | ||||
| 551-1312-00L | RNA-Biology II Number of participants limited to 16. The enrolment is done by the D-BIOL study administration. | 6 KP | 7G | S. Jonas, F. Allain, C. Beyer, U. Kutay, B. Mateescu, O. Voinnet, K. Weis | |
| Kurzbeschreibung | Introduction to the diversity of current RNA-research at all levels from structural biology to systems biology using mainly model systems like S. cerevisiae (yeast), mammalian cells. | ||||
| Lernziel | The students will obtain an overview about the diversity of current RNA-research. They will learn to design experiments and use techniques necessary to analyze different aspects of RNA biology. Through lectures and literature seminars, they will learn about the burning questions of RNA research and discuss approaches to address these questions experimentally. In practical lab projects the students will work in one of the participating laboratories. Finally, they will learn how to present and discuss their data in an appropriate manner. Student assessment is a graded semester performance based on individual performance in the laboratory, the written exam and the poster presentation. | ||||
| Literatur | Documentation and recommended literature will be provided at the beginning and during the course. | ||||
| 551-1414-00L | Molecular and Structural Biology V: Studying Macromolecules by NMR and EPR | 4 KP | 2V | F. Allain, A. D. Gossert, G. Jeschke, K. Wüthrich | |
| Kurzbeschreibung | The course provides an overview of experimental methods for the determination of structures of macromolecules at atomic resolution in solution. The two main methods used are Nuclear Magnetic Resonance (NMR) spectroscopy and Electron Paramagnetic Resonance (EPR) spectroscopy. | ||||
| Lernziel | Insight into the methodology, areas of application and limitations of these two methods for the structure determination of biological macromolecules. Practical exercises with spectra to have hands on understanding of the methodology. | ||||
| Inhalt | Part I: Methods for the determination of protein structures in solution using nuclear magnetic resonance (NMR) spectroscopy. Experimental approaches to the characterization of intramolecular dynamics of proteins. Part II: NMR methods for structurally characterizing RNA and protein-RNA complexes. Part III: EPR of biomolecules | ||||
| Literatur | 1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience. 2) Dominguez et al, Prog Nucl Magn Reson Spectrosc. 2011 Feb;58(1-2):1-61. 3) Duss O et al, Methods Enzymol. 2015;558:279-331. | ||||
| 551-1620-00L | Molecular Biology, Biophysics | 1 KP | 1K | R. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, K. Wüthrich | |
| Kurzbeschreibung | The course consists of a series of research seminars on Structural Biology and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. | ||||
| Lernziel | The goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics. | ||||
| Voraussetzungen / Besonderes | Information on the individual seminars is provided on the following websites: http://www.structuralbiology.unizh.ch/events005.asp http://www.biol.ethz.ch/dbiol-cal/index | ||||