Florian Mair: Katalogdaten im Frühjahrssemester 2023 |
| Name | Herr Dr. Florian Mair |
| Adresse | Molekulare Biomedizin, Kopf ETH Zürich, HPL G 14.1 Otto-Stern-Weg 7 8093 Zürich SWITZERLAND |
| Telefon | +41 44 633 64 81 |
| florian.mair@biol.ethz.ch | |
| Departement | Biologie |
| Beziehung | Dozent |
| Nummer | Titel | ECTS | Umfang | Dozierende | |
|---|---|---|---|---|---|
| 551-1700-00L | Introduction to Flow Cytometry  | 2 KP | 1V | F. Mair, M. Kisielow, M. Kopf | |
| Kurzbeschreibung | This course provides a broad introduction to flow cytometry. We will cover the working principles of cytometry instrumentation, and essential aspects of experimental design and data analysis. Fluorescence-based cytometry (conventional and full spectrum) as well as mass cytometry will be covered. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility. | ||||
| Lernziel | The objective of this course is to provide participants with a strong understanding of the basic principles underlying flow cytometry, equipping them to be self-sufficient for the systematic design, execution and analysis of cytometry experiments. | ||||
| Inhalt | The course is centered on explaining the working principles of cytometry instrumentation (conventional, full spectrum and mass cytometry) and associated data analysis. In detail, we will cover the following topics: - Components of Flow Cytometry Instrumentation - Signal processing - Fluorochromes, compensation, and spectral unmixing - Visualization, gating and analysis of data - Fluorescent panel design - Cytometry assays: immunophenotyping, cell signaling, etc. - Cell sorting - Mass cytometry - Computational data analysis techniques | ||||
| Skript | Updated handouts will be provided during the class. | ||||
| Literatur | The lecture will reference key literature on cytometry instrumentation, experimental design, immunophenotyping and other applications. For general reading, we recommend the following: - Maciorowski et al, Basic Multicolor Flow Cytomety, Current Protocols in Immunology 2017: https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpim.26 - Cossarizza et al, Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), European Journal of Immunology 2021: https://onlinelibrary.wiley.com/doi/10.1002/eji.202170126 - Liechti et al, An updated guide for the perplexed: cytometry in the high-dimensional era, Nature Immunology 2021: https://www.nature.com/articles/s41590-021-01006-z | ||||