Miroslav Peterek: Catalogue data in Spring Semester 2022 |
| Name | Mr Miroslav Peterek |
| Address | ScopeM ETH Zürich, HPM C 55.2 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 00 67 |
| miroslav.peterek@scopem.ethz.ch | |
| Department | Materials |
| Relationship | Lecturer |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 327-2144-00L | Microscopy Training Cryogenic Electron Microscopy  | 1 credit | 2P | M. Peterek, B. Qureshi, E. J. Barthazy Meier, S. Handschin, M. S. Lucas-Droste, P. Zeng | |
| Abstract | The introductory course on cryogenic electron microscopy (cryoEM) provides theoretical and hands-on learning for new operators, utilizing lectures, demonstrations and hands-on sessions. | ||||
| Learning objective | - Overview of cryoEM theory, instrumentation, operation and applications - Prepare cryoEM sample (vitrification using Vitrobot) - Set-up, align and operate a cryoTEM successfully and safely - Set up automated data collection - Basic processing steps to analyze/interpret the data e.g., reconstruction 3D volumes | ||||
| Content | This course introduces and gives an overview of cryoEM and its applications. At the end of the course, students will be familiar with how to prepare vitrified probe and how to use a cryoTEM to collect and analyze data for exemplary techniques: - Introduction and discussion on cryoEM and instrumentation - Lectures on cryoEM theory - Lectures on cryoEM applications - Practicals/demonstration on vitrification, grid preparation - Practicals/demonstration on data collection - Lecture and practicals/demonstration on reconstruction of 3D volumes from 2D cryoEM projections/images | ||||
| Literature | - Course slides - EM-University: (https://em-learning.com/) - Book: CryoEM Methods and Protocols edited by T Gonen, B B Nannenga - Book: Single-particle Cryo-eM of Biological Macromolecules edited by R M Glaeser, E Nogales, W Chiu | ||||
| Prerequisites / Notice | The students should fulfil one or more of these prerequisites: - Prior attendance to the ScopeM Microscopy Training TEM I - Prior TEM experience | ||||