Christian Lizak: Katalogdaten im Herbstsemester 2019 |
| Name | Herr Dr. Christian Lizak |
| Adresse | Philochem AG Libernstrasse 3 8112 Otelfingen SWITZERLAND |
| Telefon | 0435448805 |
| Departement | Chemie und Angewandte Biowissenschaften |
| Beziehung | Dozent |
| Nummer | Titel | ECTS | Umfang | Dozierende | |
|---|---|---|---|---|---|
| 535-0810-00L | Gene Technology | 2 KP | 2G | D. Neri, C. Lizak | |
| Kurzbeschreibung | The course will provide a solid overview of the science and issues in gene technology and its pharmaceutical applications. | ||||
| Lernziel | The aim of the lecture course is to provide a solid overview of gene technology, with a special focus on drug development. Topics: Antibody phage technology, DNA-encoded chemistry, protein modification technology, genome sequencing, transcriptomics, proteomics, functional genomics, principle of drug discovery. The course is suited for advanced undergraduate and early graduate students in pharmaceutical sciences or related fields. | ||||
| Inhalt | 1. Antibody phage technology The antibody molecule V genes, CDRs, basics of antibody engineering Principles of phage display Phagemid and phage vectors Antibody libraries Phage display selection methodologies Other phage libraries (peptides, globular proteins, enzymes) Alternative screening/selection methodologies DNA-encoded chemical libraries 2. Proteins: chemical modification and detection of biomolecular interactions Homo- and hetero-dimerization of proteins Chemical modifications of proteins Antibody-drug conjugates Radioactive labeling of proteins Kinetic association and dissociation constants Affinity constant: definition and its experimental measurement 3. Genomics: Applications to Human Biology Protein cloning and expression DNA sequencing Some foundations of genetic analysis Knock-out technologies Transcriptomics Proteomics Recombinant vaccines 4: Pharmaceuticals: Focus on Discovery Ligand Discovery Half-life extension Cancer therapy Gene therapy | ||||
| Skript | Skript "Gene Technology" by Prof. Dario Neri and slides of the lecture | ||||